ISFtu3
- Family IS1595
- Group IS1016
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Francisella tularensis | Francisella tularensis subsp tularensis SchuS4 |
DNA section
IS Length : 742 bp
Ends
IR Length : 17
IRL : GGGTCTAGATAACTTTAGCTTGTCAAAAATCATTGATGGAGCTATATTTT
IRR : GGGTCTAGATAACTTTAATACTAAATTTTCTTACTAAAGTTAACATGATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTTAGAA | TATAAAA | AATAAATAT | 7 |
| CTTTTAAAA | TAACTAA | ATAATAACT | 7 |
DNA sequence
GGGTCTAGATAACTTTAGCTTGTCAAAAATCATTGATGGAGCTATATTTTATCTATGTTTGGAAAGTATAAAAAACGTTCTCATATTTCAGATGCTAAAA
TTAGAGAAATCTTAAAGTGTTTTTGCTTGGATTTAACAGCAACAAACACGAGTAAAATGACTAATGTTAGTAGAGTGACAATCAATCGCTATTTTGATAG
ATTTCGTAAAATAATCTTATTATCTGATGAAAAATTCTTTGCGTCTGCTGGGGAGTTTGAGTTAGATGAGTCTTATTTTGGAGCTAAACGAGTACGAGGT
AAACGTGGTCGTGGAGCTGTAGGTAAAACACCAGTATTTGGTGTTTTATAGCGAAATGAAAACAATAAAGTCTATGTTTCTATTGTGCCAAACTGCTCTA
AAGAGTCCTTAATGCCTATAATACAGGGTAAAATACTCGAAAACAGTACAATCTATACTGATGGCTGGAAAGCGTATGATGGGCTAATTCTTAACGGTTA
TGATCACTATCGTATTTATCATTCTCATAATGAGTTTGCTCGTGGTAAAAACCATGTTAATGGTATAGAGTTATTTTGGTCGTTCTCTAAAAGAAGACTT
GCAAAATTTAATGGATTGAGTGATGATAAGTTTGTTTTACACTTAAAAGAGTGTGAGTTTAGGTGGAATAATAAAGATAATGATTTATATAAAATCATGT
TAACTTTAGTAAGAAAATTTAGTATTAAAGTTATCTAGACCC
TTAGAGAAATCTTAAAGTGTTTTTGCTTGGATTTAACAGCAACAAACACGAGTAAAATGACTAATGTTAGTAGAGTGACAATCAATCGCTATTTTGATAG
ATTTCGTAAAATAATCTTATTATCTGATGAAAAATTCTTTGCGTCTGCTGGGGAGTTTGAGTTAGATGAGTCTTATTTTGGAGCTAAACGAGTACGAGGT
AAACGTGGTCGTGGAGCTGTAGGTAAAACACCAGTATTTGGTGTTTTATAGCGAAATGAAAACAATAAAGTCTATGTTTCTATTGTGCCAAACTGCTCTA
AAGAGTCCTTAATGCCTATAATACAGGGTAAAATACTCGAAAACAGTACAATCTATACTGATGGCTGGAAAGCGTATGATGGGCTAATTCTTAACGGTTA
TGATCACTATCGTATTTATCATTCTCATAATGAGTTTGCTCGTGGTAAAAACCATGTTAATGGTATAGAGTTATTTTGGTCGTTCTCTAAAAGAAGACTT
GCAAAATTTAATGGATTGAGTGATGATAAGTTTGTTTTACACTTAAAAGAGTGTGAGTTTAGGTGGAATAATAAAGATAATGATTTATATAAAATCATGT
TAACTTTAGTAAGAAAATTTAGTATTAAAGTTATCTAGACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 684 bp | 227 aa | 54 | 737 | + | No |
Chemistry : DDE
ORF sequence :
MFGKYKKRSHISDAKIREILKCFCLDLTATNTSKMTNVSRVTINRYFDRFRKIILLSDEKFFASAGEFELDESYFGAKRVRGKRGRGAVGKTPVFGVL*R
NENNKVYVSIVPNCSKESLMPIIQGKILENSTIYTDGWKAYDGLILNGYDHYRIYHSHNEFARGKNHVNGIELFWSFSKRRLAKFNGLSDDKFVLHLKEC
EFRWNNKDNDLYKIMLTLVRKFSIKVI
NENNKVYVSIVPNCSKESLMPIIQGKILENSTIYTDGWKAYDGLILNGYDHYRIYHSHNEFARGKNHVNGIELFWSFSKRRLAKFNGLSDDKFVLHLKEC
EFRWNNKDNDLYKIMLTLVRKFSIKVI
Blast result :
Comments
ISFtu3 is 63% aa similar to ISHpa1.
There are 2 full copies of ISFtu3 in the genome of Francisella tularensis subsp tularensis at coordinates : complement (1879092..1879835) and complement (1064367..1063624) and 2 truncated copies at coordinates : (103871..104290) and complement (1546019..1546246).
There is a stop codon in the ORF at position 99 (aa).
The final stop codon is in the IRR.
In one copy (coordinates : complement (1064367..1063624)), there is a mutation in the IRR (position DNA 738, a A instead of a T) abolishing the stop codon. This results in the ORF having an extra 5 aa.
There are 2 full copies of ISFtu3 in the genome of Francisella tularensis subsp tularensis at coordinates : complement (1879092..1879835) and complement (1064367..1063624) and 2 truncated copies at coordinates : (103871..104290) and complement (1546019..1546246).
There is a stop codon in the ORF at position 99 (aa).
The final stop codon is in the IRR.
In one copy (coordinates : complement (1064367..1063624)), there is a mutation in the IRR (position DNA 738, a A instead of a T) abolishing the stop codon. This results in the ORF having an extra 5 aa.
References
1] Stephen Michell (2004) Direct submission