ISBcen1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1357 bp
Ends
IR Length : 12
IRL : taatgagATGGTCACCCCCACCCTACACGCGGGTTACGCTGTAGGGTGTT
IRR : ----tctATGGTCACCCCCTTTTTTGCAACACCGATCTTCGATGCCGTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCAGGCGGT | TTTTTATTGG | 0 |
DNA sequence
TAATGAGATGGTCACCCCCACCCTACACGCGGGTTACGCTGTAGGGTGTTTTCTTTTCTGGAGAGGCCATCATGCATGACGTAACGCTGGTTGGGATCGA
TCTGGGCAAACATTCGTTTCATCTTCACGGGCAGGATCGGCACGGCAAAGCCGTGTTCCGCAAGAAGGTGAACCGCAAGCAGCTAATCGAGTTTTTCGCC
AAGTTTCACCCCTGCACCGTGGTCATGGAGGCCTGCGCCGGAGCACATTACATGGCGCGCCAATTGGCGGGCTGGGGCCATCAGGTCAAACTGATCTCGC
CACAGTTCGTGCGGCCCTTCGTCAAGAGTAACAAAAACGACTTCGTGGACGCCGAAGCGATCTGCGAGGCAGCCTCACGCCCATCTATGCGATTTGTGAC
GCCGAAGACGGAGTCGCAGCAGATTTTGTCAGCTTTGCACCGCGTGCGTGATTCTCTGGTGCGGGATCGAGTGAAGACGGCCAACCAATTACACGGGTTC
CTGCTCGAATTCGGCATCAGCCTGCCAGTCGGGCAAGCCGTGATCAAGCGATTGCCGGCTGTGCTGGCCGAGCATGCGCTGCCGCCCCGGCTAGTGGCGA
TCCTCGAGCGCTTGCACGGCCATTTCAAGTACCTGAGTGAGCAGATCAGTGAGATTGACGCCGAGATGGCTCGGCAACTAGCCGACGATGAGCTGGGGCA
ATGCCTCTTGAGCATTCCTTGCGTTGGCCCGATTACGGCAAGCGTGCTGGCCGCGGAAATGGGCGACGGCAAGCAATACGGCTGCAGTCGCGACTTTGCC
GCATCGGTCGGGCTCGTGCCACGCCAGTACAGCACGGGCGGCAAAAGCAATCTGCTGGGCATCAGCAAACGCGGGGACAAGAATATCCGGCGGCTACTGG
TGCAGTGCGCCCGAGTCTACATGCAGCAACTGGAACGGCAAAGCGGCCGGCTTGCCGATTGGGTCCGGACCATGCTGGCGCGTCGGCACTCAAACGTTGT
GGCTTGCGCGCTGGCAAATAAGCTGGCTCGTACGGCATGGGCACTTGTTACCAGCCGTACCACCTTCGATGCGCGGTCGGCAGGTATGCCGGCCTGATTA
ACCGACTTCGGCTTGTTGCTGTAAGAATCACCCACCTGGTTTTGCGAACGCTGAAATTTGATGACGTGAACGGCACACCGGCCTGACCGAGAACCTGTCA
TAAAAATTGGCTTCTGAAGCCGCGCGGTTTTTTAGGACGGTCAGGCGCGACTCTCATCGTGGCGCGGAGAGCAATCCCCACAACGACGCCGGATAGATTT
AGGCAAGCCATACACGGCATCGAAGATCGGTGTTGCAAAAAAGGGGGTGACCATAGA
TCTGGGCAAACATTCGTTTCATCTTCACGGGCAGGATCGGCACGGCAAAGCCGTGTTCCGCAAGAAGGTGAACCGCAAGCAGCTAATCGAGTTTTTCGCC
AAGTTTCACCCCTGCACCGTGGTCATGGAGGCCTGCGCCGGAGCACATTACATGGCGCGCCAATTGGCGGGCTGGGGCCATCAGGTCAAACTGATCTCGC
CACAGTTCGTGCGGCCCTTCGTCAAGAGTAACAAAAACGACTTCGTGGACGCCGAAGCGATCTGCGAGGCAGCCTCACGCCCATCTATGCGATTTGTGAC
GCCGAAGACGGAGTCGCAGCAGATTTTGTCAGCTTTGCACCGCGTGCGTGATTCTCTGGTGCGGGATCGAGTGAAGACGGCCAACCAATTACACGGGTTC
CTGCTCGAATTCGGCATCAGCCTGCCAGTCGGGCAAGCCGTGATCAAGCGATTGCCGGCTGTGCTGGCCGAGCATGCGCTGCCGCCCCGGCTAGTGGCGA
TCCTCGAGCGCTTGCACGGCCATTTCAAGTACCTGAGTGAGCAGATCAGTGAGATTGACGCCGAGATGGCTCGGCAACTAGCCGACGATGAGCTGGGGCA
ATGCCTCTTGAGCATTCCTTGCGTTGGCCCGATTACGGCAAGCGTGCTGGCCGCGGAAATGGGCGACGGCAAGCAATACGGCTGCAGTCGCGACTTTGCC
GCATCGGTCGGGCTCGTGCCACGCCAGTACAGCACGGGCGGCAAAAGCAATCTGCTGGGCATCAGCAAACGCGGGGACAAGAATATCCGGCGGCTACTGG
TGCAGTGCGCCCGAGTCTACATGCAGCAACTGGAACGGCAAAGCGGCCGGCTTGCCGATTGGGTCCGGACCATGCTGGCGCGTCGGCACTCAAACGTTGT
GGCTTGCGCGCTGGCAAATAAGCTGGCTCGTACGGCATGGGCACTTGTTACCAGCCGTACCACCTTCGATGCGCGGTCGGCAGGTATGCCGGCCTGATTA
ACCGACTTCGGCTTGTTGCTGTAAGAATCACCCACCTGGTTTTGCGAACGCTGAAATTTGATGACGTGAACGGCACACCGGCCTGACCGAGAACCTGTCA
TAAAAATTGGCTTCTGAAGCCGCGCGGTTTTTTAGGACGGTCAGGCGCGACTCTCATCGTGGCGCGGAGAGCAATCCCCACAACGACGCCGGATAGATTT
AGGCAAGCCATACACGGCATCGAAGATCGGTGTTGCAAAAAAGGGGGTGACCATAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 72 | 1097 | + | No |
Chemistry : DEDD
ORF sequence :
MHDVTLVGIDLGKHSFHLHGQDRHGKAVFRKKVNRKQLIEFFAKFHPCTVVMEACAGAHYMARQLAGWGHQVKLISPQFVRPFVKSNKNDFVDAEAICEA
ASRPSMRFVTPKTESQQILSALHRVRDSLVRDRVKTANQLHGFLLEFGISLPVGQAVIKRLPAVLAEHALPPRLVAILERLHGHFKYLSEQISEIDAEMA
RQLADDELGQCLLSIPCVGPITASVLAAEMGDGKQYGCSRDFAASVGLVPRQYSTGGKSNLLGISKRGDKNIRRLLVQCARVYMQQLERQSGRLADWVRT
MLARRHSNVVACALANKLARTAWALVTSRTTFDARSAGMPA
ASRPSMRFVTPKTESQQILSALHRVRDSLVRDRVKTANQLHGFLLEFGISLPVGQAVIKRLPAVLAEHALPPRLVAILERLHGHFKYLSEQISEIDAEMA
RQLADDELGQCLLSIPCVGPITASVLAAEMGDGKQYGCSRDFAASVGLVPRQYSTGGKSNLLGISKRGDKNIRRLLVQCARVYMQQLERQSGRLADWVRT
MLARRHSNVVACALANKLARTAWALVTSRTTFDARSAGMPA
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, asuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase protein is 72, 69 and 41% identical to those of ISAzvi4, IS1328 and IS1111 respectively.
The transposase protein is 72, 69 and 41% identical to those of ISAzvi4, IS1328 and IS1111 respectively.
References
1] The Welcome Trust Sanger Institute http://www.sanger.ac.uk
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384