ISCc4
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE005940 | ND | Caulobacter crescentus | Caulobacter crescentus CB15 |
DNA section
IS Length : 1174 bp
Ends
IR Length : 12
IRL : tgatgggGTGGACGGCCCCTGTGTCCGGCGTCGAGTTCCCCATAGGGTGG
IRR :
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGATGGGGTGGACGGCCCCTGTGTCCGGCGTCGAGTTCCCCATAGGGTGGTCGTTGCTTGCGCGGCCACAGAACAGGAACCGTCCACTATGATGACTACC
ACAATCGGCCTCGACCTCGCCAAGAGTGTCTTCCAGGTTCACGGCGTCAGTTCAGAGGGGCAGGTCACTGTCCGTCGGAAGCTGCGCCGCGGCGAACTGG
TGAGTTTCTTTCGGGATTTGCCCCCGTGCCTGATCGGCATGGAGGCTTGCGCGACCGCCCACTACTGGGCCCGCGAACTGTCAGCCCTGGGCCATGAGGT
GCGGCTGATGCCGCCAAGCTATGTGAAGGCCTATCTCCGTCGCCAGAAGAACGATGCGGCTGACGCCGAGGCGATCTGCGAGGCCGTCACCCGACCGACG
ATGCGGTTCGTGCCGGTGAAGAGTGCGGAGCGTCAGGCAATCCTGACCCTGCACCGGACCCGAGAGCTTTTGGTGCGCCAGAAGACGACGTTGATCCGCG
CGATTAGAGGCCACTGCGCTGAGTTTGGTCTGGTGGTCCCTCAAGGCGCGCGCCGTGTGTTCGACCTGCTGAACTTGATGCGGGAAGCGAGTTCCTCGTC
ACTGCCGGAGACAGCCATGGTGACCATGGAGATGCTCGGTGCCCAGATGGAGGCGTTGGCGCTGCAGATCCGCACGCTCGAGCGACGTCTGCTCGCCTGG
CATCGCGAAAACCAGACCAGCCAGCGCCTCGCCACTATCCCAGGGGTCGGGATCATCACATCGACCGCGCTGGCCGCCAGCATCAGCGACCCACGTCTGT
TCCGGTCAGGTCGGGAATTCGCGGCCTTCCTTGGCCTGGTCCCTCGGCAGAACTCATCCGGCGGCAAAGATCGCTTGGGCCGCATAACCAAGAAGGGCGA
TGGCTATCTGCGCAAGCTCCTGGTGGTCGGGGCGACGTCGGTGATCCGCCGCGCGCGGACCGGGGTGACGCCATCCGCCGGCTGGGTCCAGTCGTTGCTC
GAGCGTCGGCCATCCCGCGTCGTCACGGTCGCCATGGCCAACAAGACCGCGCGGATCGCCTGGGCGGTCATGACGCGCGGCGAGAGCTATCGGGCCATGG
CCACGGTCTGAATGAGATCGCGGCGGACCGTCGGTCCGCCGCTCCGAACTGGAAGGGTGCAAATGGGTTGATGA
ACAATCGGCCTCGACCTCGCCAAGAGTGTCTTCCAGGTTCACGGCGTCAGTTCAGAGGGGCAGGTCACTGTCCGTCGGAAGCTGCGCCGCGGCGAACTGG
TGAGTTTCTTTCGGGATTTGCCCCCGTGCCTGATCGGCATGGAGGCTTGCGCGACCGCCCACTACTGGGCCCGCGAACTGTCAGCCCTGGGCCATGAGGT
GCGGCTGATGCCGCCAAGCTATGTGAAGGCCTATCTCCGTCGCCAGAAGAACGATGCGGCTGACGCCGAGGCGATCTGCGAGGCCGTCACCCGACCGACG
ATGCGGTTCGTGCCGGTGAAGAGTGCGGAGCGTCAGGCAATCCTGACCCTGCACCGGACCCGAGAGCTTTTGGTGCGCCAGAAGACGACGTTGATCCGCG
CGATTAGAGGCCACTGCGCTGAGTTTGGTCTGGTGGTCCCTCAAGGCGCGCGCCGTGTGTTCGACCTGCTGAACTTGATGCGGGAAGCGAGTTCCTCGTC
ACTGCCGGAGACAGCCATGGTGACCATGGAGATGCTCGGTGCCCAGATGGAGGCGTTGGCGCTGCAGATCCGCACGCTCGAGCGACGTCTGCTCGCCTGG
CATCGCGAAAACCAGACCAGCCAGCGCCTCGCCACTATCCCAGGGGTCGGGATCATCACATCGACCGCGCTGGCCGCCAGCATCAGCGACCCACGTCTGT
TCCGGTCAGGTCGGGAATTCGCGGCCTTCCTTGGCCTGGTCCCTCGGCAGAACTCATCCGGCGGCAAAGATCGCTTGGGCCGCATAACCAAGAAGGGCGA
TGGCTATCTGCGCAAGCTCCTGGTGGTCGGGGCGACGTCGGTGATCCGCCGCGCGCGGACCGGGGTGACGCCATCCGCCGGCTGGGTCCAGTCGTTGCTC
GAGCGTCGGCCATCCCGCGTCGTCACGGTCGCCATGGCCAACAAGACCGCGCGGATCGCCTGGGCGGTCATGACGCGCGGCGAGAGCTATCGGGCCATGG
CCACGGTCTGAATGAGATCGCGGCGGACCGTCGGTCCGCCGCTCCGAACTGGAAGGGTGCAAATGGGTTGATGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 89 | 1111 | + | No |
Chemistry : DEDD
ORF sequence :
MMTTTIGLDLAKSVFQVHGVSSEGQVTVRRKLRRGELVSFFRDLPPCLIGMEACATAHYWARELSALGHEVRLMPPSYVKAYLRRQKNDAADAEAICEAV
TRPTMRFVPVKSAERQAILTLHRTRELLVRQKTTLIRAIRGHCAEFGLVVPQGARRVFDLLNLMREASSSSLPETAMVTMEMLGAQMEALALQIRTLERR
LLAWHRENQTSQRLATIPGVGIITSTALAASISDPRLFRSGREFAAFLGLVPRQNSSGGKDRLGRITKKGDGYLRKLLVVGATSVIRRARTGVTPSAGWV
QSLLERRPSRVVTVAMANKTARIAWAVMTRGESYRAMATV
TRPTMRFVPVKSAERQAILTLHRTRELLVRQKTTLIRAIRGHCAEFGLVVPQGARRVFDLLNLMREASSSSLPETAMVTMEMLGAQMEALALQIRTLERR
LLAWHRENQTSQRLATIPGVGIITSTALAASISDPRLFRSGREFAAFLGLVPRQNSSGGKDRLGRITKKGDGYLRKLLVVGATSVIRRARTGVTPSAGWV
QSLLERRPSRVVTVAMANKTARIAWAVMTRGESYRAMATV
Blast result :
Comments
The right-hand end of this IS, including IRr, is missing due to the insertion of IS511, so the left-hand end has been defined by analogy with other IS1111 family elements, assuming 7 nt separate IRl from the left-hand end of the element.
The transposase protein is 37% identical to that of IS1111
The transposase protein is 37% identical to that of IS1111
References
1] Nierman et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 4136-4141
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384