ISMmg2
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Magnetospirillum magnetotacticum | Magnetospirillum magnetotacticum MS-1 |
DNA section
IS Length : 1381 bp
Ends
IR Length : 15
IRL : gatgggGTGGATGGCTCCCGCTCCGACTGACGGCATCTGAGTGCCAGGAT
IRR : ---tgtGTGGATGGCTCCCGCATTGCAAGTGGCGAATCGAGCTTCTGGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GATGGGGTGGATGGCTCCCGCTCCGACTGACGGCATCTGAGTGCCAGGATGTGGTTGCTGTCGAAGCAGCCACGCAGATGCAGGAGCCATCCCCATGGAG
ATCATCACCGTCGGCATCGATCTAGCCAAGAGCATCTTCCAGGTTCACGCCGTCGACGCAGCTGGGCACGTCGTCGTTCGCAAGGCTCTGAGGCGGGCGC
AGGTCGTGCCGTTCTTCGCCAAGCTGCCGCGCTGCCTGGTCGGCATGGAGGCGTGCGGCACAGCGCACCATTGGGCCCGGGAACTCATGAGGCTCGGCCA
TGACGTGCGGCTGATGCCGCCAGCTTACGTGAAGCCGTACGTCAAGCGCGGCAAAACCGATGCGAATGATGCCGCTGCCATCTGCGAGGCGGTGACACGC
CCGAGCATGCGCTTCGTGCCGGTGAAGTCGACCGAGCAGCAGGCGGCGCTGGCCCTGCACCGGACGCGCGACCTGCTGGTCAAGCAACGCACGCAGCTGG
TGAACATGATCCGCGGTCTGCTCGCCGAGTTCGGGATCGAGATGGCGCGGGGCCTGCGCCATGCGCTCGAACTGGCAGCTCGGCTCTCAGCCGGAGACGC
GGCCGAGGTGCCGCCCCTGGCCCAGCGCGTAGTGAATGGGCTGGCCGATCAGATCGGCGCCGTGCAGATCCAGCTCACCCGGCTGGAGAAGGAGTTGCTG
GCCTGGCATCGGGACTGCGACCTGTCACAGCGCCTGGCGACGATCCCGGGTGTCGGCATCGTCTCGGCGACCGCGCTGGCCGCCTCGGTGAGCGAGCCCG
AGCGCTTCCGCTCCGGTCGGCAGTTCGCGGCCTCGTTGGGTCTGACACCGCTGCAGAACTGCAGCGGCGGCAAGGAGCGCCTGGGTCGGATCTCGCGCAT
GGGCGACCGTTACCTGCGCCGCCTGCTCGTGGTCGGCATGACCTCTCTGATCCGGCGCGCGAAGGCGACGCCGACTTCGGTCGACCCGCGGCTGCCGGCG
CTGCTGCAGCGCAAGCCGGTGCGCGTGGTGACTGTTGCGGCGGCCAATCGCACGGCGCGGGTCGCCTGGGCGATCATGACCCGCGGTGGCACCTACCGCG
CACCAGCGGCCATGGCCGCCTGACAGCACGGCGAGGAGACTTTGCCAGGTTGCGAGGACGATGAGAGTTGATGGCGAACCGGTCAGACCGGGAGCCGGGA
CACCCCGCCGAATGTCCAGGGCGTCATAGCCCGCTAAGCAGAGTGGGACCCAGCTCGCGGATACCATCAGGGCCAGCAGTCAACATGCACGACTGCATCA
ACAGGCCGGACACAAGACTGCACCCGACCAACGCGCCAGAAGCTCGATTCGCCACTTGCAATGCGGGAGCCATCCACACA
ATCATCACCGTCGGCATCGATCTAGCCAAGAGCATCTTCCAGGTTCACGCCGTCGACGCAGCTGGGCACGTCGTCGTTCGCAAGGCTCTGAGGCGGGCGC
AGGTCGTGCCGTTCTTCGCCAAGCTGCCGCGCTGCCTGGTCGGCATGGAGGCGTGCGGCACAGCGCACCATTGGGCCCGGGAACTCATGAGGCTCGGCCA
TGACGTGCGGCTGATGCCGCCAGCTTACGTGAAGCCGTACGTCAAGCGCGGCAAAACCGATGCGAATGATGCCGCTGCCATCTGCGAGGCGGTGACACGC
CCGAGCATGCGCTTCGTGCCGGTGAAGTCGACCGAGCAGCAGGCGGCGCTGGCCCTGCACCGGACGCGCGACCTGCTGGTCAAGCAACGCACGCAGCTGG
TGAACATGATCCGCGGTCTGCTCGCCGAGTTCGGGATCGAGATGGCGCGGGGCCTGCGCCATGCGCTCGAACTGGCAGCTCGGCTCTCAGCCGGAGACGC
GGCCGAGGTGCCGCCCCTGGCCCAGCGCGTAGTGAATGGGCTGGCCGATCAGATCGGCGCCGTGCAGATCCAGCTCACCCGGCTGGAGAAGGAGTTGCTG
GCCTGGCATCGGGACTGCGACCTGTCACAGCGCCTGGCGACGATCCCGGGTGTCGGCATCGTCTCGGCGACCGCGCTGGCCGCCTCGGTGAGCGAGCCCG
AGCGCTTCCGCTCCGGTCGGCAGTTCGCGGCCTCGTTGGGTCTGACACCGCTGCAGAACTGCAGCGGCGGCAAGGAGCGCCTGGGTCGGATCTCGCGCAT
GGGCGACCGTTACCTGCGCCGCCTGCTCGTGGTCGGCATGACCTCTCTGATCCGGCGCGCGAAGGCGACGCCGACTTCGGTCGACCCGCGGCTGCCGGCG
CTGCTGCAGCGCAAGCCGGTGCGCGTGGTGACTGTTGCGGCGGCCAATCGCACGGCGCGGGTCGCCTGGGCGATCATGACCCGCGGTGGCACCTACCGCG
CACCAGCGGCCATGGCCGCCTGACAGCACGGCGAGGAGACTTTGCCAGGTTGCGAGGACGATGAGAGTTGATGGCGAACCGGTCAGACCGGGAGCCGGGA
CACCCCGCCGAATGTCCAGGGCGTCATAGCCCGCTAAGCAGAGTGGGACCCAGCTCGCGGATACCATCAGGGCCAGCAGTCAACATGCACGACTGCATCA
ACAGGCCGGACACAAGACTGCACCCGACCAACGCGCCAGAAGCTCGATTCGCCACTTGCAATGCGGGAGCCATCCACACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 96 | 1124 | + | No |
Chemistry : DEDD
ORF sequence :
MEIITVGIDLAKSIFQVHAVDAAGHVVVRKALRRAQVVPFFAKLPRCLVGMEACGTAHHWARELMRLGHDVRLMPPAYVKPYVKRGKTDANDAAAICEAV
TRPSMRFVPVKSTEQQAALALHRTRDLLVKQRTQLVNMIRGLLAEFGIEMARGLRHALELAARLSAGDAAEVPPLAQRVVNGLADQIGAVQIQLTRLEKE
LLAWHRDCDLSQRLATIPGVGIVSATALAASVSEPERFRSGRQFAASLGLTPLQNCSGGKERLGRISRMGDRYLRRLLVVGMTSLIRRAKATPTSVDPRL
PALLQRKPVRVVTVAAANRTARVAWAIMTRGGTYRAPAAMAA
TRPSMRFVPVKSTEQQAALALHRTRDLLVKQRTQLVNMIRGLLAEFGIEMARGLRHALELAARLSAGDAAEVPPLAQRVVNGLADQIGAVQIQLTRLEKE
LLAWHRDCDLSQRLATIPGVGIVSATALAASVSEPERFRSGRQFAASLGLTPLQNCSGGKERLGRISRMGDRYLRRLLVVGMTSLIRRAKATPTSVDPRL
PALLQRKPVRVVTVAAANRTARVAWAIMTRGGTYRAPAAMAA
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end so that the first nt of the above sequence matches the one adjacent to the left-hand end of the above sequence.
The transposase protein is 82% identical to that of ISMlo3 and 42% identical to that of IS1111.
The transposase protein is 82% identical to that of ISMlo3 and 42% identical to that of IS1111.
References
1] US Department of Energy Joint Genome Institute http://jgi.doe.gov/JGI_microbial/html/
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384