ISBcen3
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1413 bp
Ends
IR Length : 13
IRL : taatggaATGGACGCCCCCGCCCGGCACGGCATCGATGTGCCAAAGTGGA
IRR : ----tacATGGACGCCCCCGTTTTGCCAGGTTCGCGGTGAGTGACGGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGACCTCTGC | TGACTTCTCG | 0 | |
| CGACCTCTGC | TGACTTCTCG | 0 |
DNA sequence
TAATGGAATGGACGCCCCCGCCCGGCACGGCATCGATGTGCCAAAGTGGAAGTGTCAGCCACCACTTAACCAGAGGGAACGCTCATCATGAAGGTTACGA
CCGTTGGTATCGATCTGGCAAAGAACGTCTTTCAAATCCACGGCGTCGACGCGCACGGCAAAGCCGTCCTCAAGAAGCAGATCCGGCGCGATCAATTAAT
GCTGTTCTTCACGAATCTGCCAGCGTGCTTGATCGGCATGGAGGCCTGTGCCAGCGCGCATTACTGGGCACGACGCGTTCAAGAATGCGGACACACCGTT
CGACTAATGGCTCCGCAATTCGTTAAACCCTACGTCAAGACCAACAAGAACGATGCGGCCGATGCCGAAGCAATTTGCGAAGCCGTCGCTCGGCCCAACA
TGCGCTTCGTCCCCATCAAGACCGAATCGCAGCAGGCCGTCCTCGCCATACATCGCGCACGTCAGAGTTTCGTCAAGGCGCGCACCGCGCAAGCTAATCA
GATTCGTGGGCTCCTCGCCGAATTCGGCCTGGTGATTCCCCAAGGTATCGCCCACATCGCGCAACGCGTACCCGAACTGCTCGACTCCGACACTTGCGCC
TTGCCAGGCCCGTTTCGTCTCCTTGTGCTGCGCCTGATGGAGCATCTCAAGCTGCTCGATCAGCACGTTCGAGAACTCGAGACGCAAATTCAGACCTGGC
ACCGCGACAATCCCGCCAGCCGCAAACTTGCCGAGATTCCCGGCGTCGGGCCAATCACGGCAAGCGCACTGGTCGCGTCGATCGGCGATGCCAAGAACTT
CGAGAACGGCCGTCAACTGGCCGCTTGGCTTGGGCTGGTCCCTCGGCAACATTCCAGCGGCGGCAAATCGACCTTGCTGGGCATGAGCAAGCGAGGCGAC
TCCTACCTGCGCACGCTGCTCATCCACGGCGCGCGATCGGCCATCCTAGCCGCACAACGAAAGCCCGACACCGATAGCTGGCTGAGCCGCCTGCTAGGCC
GACGCCATCCCAACATCGCTGCCGTAGCCCTGGCCAGCAAGAATGCACGCGTCATATGGGCGTTGCTGGCTCACAACCAGCAGTTCCGACCGGGTTATGC
GACTGCCGCGGCAGTCGCATAACCCGGTCGGCTTGCAGTCGAACAAGACCGCTGCGACAAACTTTAGGAGCGACAAACCACCCGATTGCTCAGGCAAGCA
ATGCAGTGATGGCGAGACAGGTCAGACCGTGGCCGGCAAAACCCATAGAAGTCAGAGCACCTCAAGTGCGATCTGCTGATTGAGGAGCTGGCCAGCGTAT
TCCATCAGGGACAGCAGGCGTACGCCTGACATCAAAGTCCGGATGTATGGCTGCAATCGATGCCTTCGAACCGTCACTCACCGCGAACCTGGCAAAACGG
GGGCGTCCATGTA
CCGTTGGTATCGATCTGGCAAAGAACGTCTTTCAAATCCACGGCGTCGACGCGCACGGCAAAGCCGTCCTCAAGAAGCAGATCCGGCGCGATCAATTAAT
GCTGTTCTTCACGAATCTGCCAGCGTGCTTGATCGGCATGGAGGCCTGTGCCAGCGCGCATTACTGGGCACGACGCGTTCAAGAATGCGGACACACCGTT
CGACTAATGGCTCCGCAATTCGTTAAACCCTACGTCAAGACCAACAAGAACGATGCGGCCGATGCCGAAGCAATTTGCGAAGCCGTCGCTCGGCCCAACA
TGCGCTTCGTCCCCATCAAGACCGAATCGCAGCAGGCCGTCCTCGCCATACATCGCGCACGTCAGAGTTTCGTCAAGGCGCGCACCGCGCAAGCTAATCA
GATTCGTGGGCTCCTCGCCGAATTCGGCCTGGTGATTCCCCAAGGTATCGCCCACATCGCGCAACGCGTACCCGAACTGCTCGACTCCGACACTTGCGCC
TTGCCAGGCCCGTTTCGTCTCCTTGTGCTGCGCCTGATGGAGCATCTCAAGCTGCTCGATCAGCACGTTCGAGAACTCGAGACGCAAATTCAGACCTGGC
ACCGCGACAATCCCGCCAGCCGCAAACTTGCCGAGATTCCCGGCGTCGGGCCAATCACGGCAAGCGCACTGGTCGCGTCGATCGGCGATGCCAAGAACTT
CGAGAACGGCCGTCAACTGGCCGCTTGGCTTGGGCTGGTCCCTCGGCAACATTCCAGCGGCGGCAAATCGACCTTGCTGGGCATGAGCAAGCGAGGCGAC
TCCTACCTGCGCACGCTGCTCATCCACGGCGCGCGATCGGCCATCCTAGCCGCACAACGAAAGCCCGACACCGATAGCTGGCTGAGCCGCCTGCTAGGCC
GACGCCATCCCAACATCGCTGCCGTAGCCCTGGCCAGCAAGAATGCACGCGTCATATGGGCGTTGCTGGCTCACAACCAGCAGTTCCGACCGGGTTATGC
GACTGCCGCGGCAGTCGCATAACCCGGTCGGCTTGCAGTCGAACAAGACCGCTGCGACAAACTTTAGGAGCGACAAACCACCCGATTGCTCAGGCAAGCA
ATGCAGTGATGGCGAGACAGGTCAGACCGTGGCCGGCAAAACCCATAGAAGTCAGAGCACCTCAAGTGCGATCTGCTGATTGAGGAGCTGGCCAGCGTAT
TCCATCAGGGACAGCAGGCGTACGCCTGACATCAAAGTCCGGATGTATGGCTGCAATCGATGCCTTCGAACCGTCACTCACCGCGAACCTGGCAAAACGG
GGGCGTCCATGTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1035 bp | 344 aa | 88 | 1122 | + | No |
Chemistry : DEDD
ORF sequence :
MKVTTVGIDLAKNVFQIHGVDAHGKAVLKKQIRRDQLMLFFTNLPACLIGMEACASAHYWARRVQECGHTVRLMAPQFVKPYVKTNKNDAADAEAICEAV
ARPNMRFVPIKTESQQAVLAIHRARQSFVKARTAQANQIRGLLAEFGLVIPQGIAHIAQRVPELLDSDTCALPGPFRLLVLRLMEHLKLLDQHVRELETQ
IQTWHRDNPASRKLAEIPGVGPITASALVASIGDAKNFENGRQLAAWLGLVPRQHSSGGKSTLLGMSKRGDSYLRTLLIHGARSAILAAQRKPDTDSWLS
RLLGRRHPNIAAVALASKNARVIWALLAHNQQFRPGYATAAAVA
ARPNMRFVPIKTESQQAVLAIHRARQSFVKARTAQANQIRGLLAEFGLVIPQGIAHIAQRVPELLDSDTCALPGPFRLLVLRLMEHLKLLDQHVRELETQ
IQTWHRDNPASRKLAEIPGVGPITASALVASIGDAKNFENGRQLAAWLGLVPRQHSSGGKSTLLGMSKRGDSYLRTLLIHGARSAILAAQRKPDTDSWLS
RLLGRRHPNIAAVALASKNARVIWALLAHNQQFRPGYATAAAVA
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
There are 2 copies of ISBcen3 in the Burkholderia cenocepacia J2315 genome. The transposase protein is 43% identical to that of IS1111.
The two copies are inserted at the same place in two introns.
By analogy with IS4321, ISBcen3 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
There are 2 copies of ISBcen3 in the Burkholderia cenocepacia J2315 genome. The transposase protein is 43% identical to that of IS1111.
The two copies are inserted at the same place in two introns.
By analogy with IS4321, ISBcen3 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] The Welcome Trust Sanger Institute http://www.sanger.ac.uk
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384