ISBcen2
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1363 bp
Ends
IR Length : 14
IRL : taataggATGGTCAGCCCGATCCTCACTCAGTGGACTACGCTGAGTGAGG
IRR : ----tctATGGTCAGCCCGATTTTTGCAAGCGAGGTGGTGTTGACCTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCCAGGGGT | TTTTTGGCAG | 0 |
DNA sequence
TAATAGGATGGTCAGCCCGATCCTCACTCAGTGGACTACGCTGAGTGAGGATTTTGTCCTTCTGGAGGACCATCATGGACACGGTATCGCTGATCGGAGT
CGATCTCGGCAAACACTGCTTCCACCTGCATGGGCAGGATGCGTCAGGCAGGATGGTGTTCCGTAAGAAGCTCACGCGCAGCCAGATGTTTACGCTGCTG
GGCAATTTCCCGAGTTGTACGGTGGTCATGGAGGCCTGCGCGGGTGCTCACTGGATCGCCCGCCGACTTCAGACGCTGGGCCACGAGGCCAAGCTGATTT
CCCCTCAGTTCGTCAAACCGTTCCGGCAAGGCAACAAGAACGATTTCGCGGACGCCCAGGCGATCTGCGAAGCAGCTTCGCGTCCGAGCATGCGTTTCGC
GAGTCCGCACAACGAAGCTCAGCAGATCGTTTCAGCCTTGCACCGCGTGCGCGAGGGCCTTGTGCGTCAGCGCACCTGCACGATCAATCAGATTCATGCG
TTTCTGTTGGAGTTCGGCATCAGCTTGCCGCGCGGTATGGCGGTGATCCGACGGCTGCCCGCCGTGCTGGAGGCAGAATCGTTGCCGCCGAGACTGGTGA
GCGTGCTCGAACGTCTGCACGCGCACTTCAAGTATCTGGACGAGCAGATCCATCAGCTCGAACATGAGTTGCTTTCCCAGTTACACGAGGACGAACGCAG
CGAACGGCTGCTCGAGATTCCGGGCATTGGCCCCATGACTGCCAGCGTGTTGATGTCGGAGTTGGGCGATGCGCGTCAATATGGTTCGGCAAGGCAGTTT
GCGGCGTCGGTCGGGCTGGTGCCGCGGCAGTACAGCACCGGCGGCAAACCAACGCTACTCGGCATCAGCAAGCGCGGGGACAAGGAATTGCGGCGGCTGC
TGGTGCAATGTGCGCGGACCATCATGCAGCGCATCGAGCACCGCACGGATGCATTGGGCGTCTGGATTCGCAGCCTGTTGGCGCGACGACATTCGAACGT
AGTGGCCTGTGCCTTGGCCAATAAATTGGCGAGGATCGCGTCGGCCATCCTCGCCAAAGGGACACACTACCGAAGCATCGAGGCTGTTCCTGCAGTCTGA
CGCTTCCTGTCGCTTACTGCTTCAGTTGCAGTAGTCACACCAGGTTTTGCGACGCGAGATACGTGAAGGCATAAACGACTCAACGGCCGGGCAAGTAACC
TGACACAAAAAACAGCATCTCAATGCTGAGGGGTTTTTGAGGTTTGCCCGGCGCGGCTCCCATCATGGGGCGGGATCTTTCCCATCCGCGACCCCGGATA
GATTCGAGCAAGTCCACTTTAGGTCAACACCACCTCGCTTGCAAAAATCGGGCTGACCATAGA
CGATCTCGGCAAACACTGCTTCCACCTGCATGGGCAGGATGCGTCAGGCAGGATGGTGTTCCGTAAGAAGCTCACGCGCAGCCAGATGTTTACGCTGCTG
GGCAATTTCCCGAGTTGTACGGTGGTCATGGAGGCCTGCGCGGGTGCTCACTGGATCGCCCGCCGACTTCAGACGCTGGGCCACGAGGCCAAGCTGATTT
CCCCTCAGTTCGTCAAACCGTTCCGGCAAGGCAACAAGAACGATTTCGCGGACGCCCAGGCGATCTGCGAAGCAGCTTCGCGTCCGAGCATGCGTTTCGC
GAGTCCGCACAACGAAGCTCAGCAGATCGTTTCAGCCTTGCACCGCGTGCGCGAGGGCCTTGTGCGTCAGCGCACCTGCACGATCAATCAGATTCATGCG
TTTCTGTTGGAGTTCGGCATCAGCTTGCCGCGCGGTATGGCGGTGATCCGACGGCTGCCCGCCGTGCTGGAGGCAGAATCGTTGCCGCCGAGACTGGTGA
GCGTGCTCGAACGTCTGCACGCGCACTTCAAGTATCTGGACGAGCAGATCCATCAGCTCGAACATGAGTTGCTTTCCCAGTTACACGAGGACGAACGCAG
CGAACGGCTGCTCGAGATTCCGGGCATTGGCCCCATGACTGCCAGCGTGTTGATGTCGGAGTTGGGCGATGCGCGTCAATATGGTTCGGCAAGGCAGTTT
GCGGCGTCGGTCGGGCTGGTGCCGCGGCAGTACAGCACCGGCGGCAAACCAACGCTACTCGGCATCAGCAAGCGCGGGGACAAGGAATTGCGGCGGCTGC
TGGTGCAATGTGCGCGGACCATCATGCAGCGCATCGAGCACCGCACGGATGCATTGGGCGTCTGGATTCGCAGCCTGTTGGCGCGACGACATTCGAACGT
AGTGGCCTGTGCCTTGGCCAATAAATTGGCGAGGATCGCGTCGGCCATCCTCGCCAAAGGGACACACTACCGAAGCATCGAGGCTGTTCCTGCAGTCTGA
CGCTTCCTGTCGCTTACTGCTTCAGTTGCAGTAGTCACACCAGGTTTTGCGACGCGAGATACGTGAAGGCATAAACGACTCAACGGCCGGGCAAGTAACC
TGACACAAAAAACAGCATCTCAATGCTGAGGGGTTTTTGAGGTTTGCCCGGCGCGGCTCCCATCATGGGGCGGGATCTTTCCCATCCGCGACCCCGGATA
GATTCGAGCAAGTCCACTTTAGGTCAACACCACCTCGCTTGCAAAAATCGGGCTGACCATAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 75 | 1100 | + | No |
Chemistry : DEDD
ORF sequence :
MDTVSLIGVDLGKHCFHLHGQDASGRMVFRKKLTRSQMFTLLGNFPSCTVVMEACAGAHWIARRLQTLGHEAKLISPQFVKPFRQGNKNDFADAQAICEA
ASRPSMRFASPHNEAQQIVSALHRVREGLVRQRTCTINQIHAFLLEFGISLPRGMAVIRRLPAVLEAESLPPRLVSVLERLHAHFKYLDEQIHQLEHELL
SQLHEDERSERLLEIPGIGPMTASVLMSELGDARQYGSARQFAASVGLVPRQYSTGGKPTLLGISKRGDKELRRLLVQCARTIMQRIEHRTDALGVWIRS
LLARRHSNVVACALANKLARIASAILAKGTHYRSIEAVPAV
ASRPSMRFASPHNEAQQIVSALHRVREGLVRQRTCTINQIHAFLLEFGISLPRGMAVIRRLPAVLEAESLPPRLVSVLERLHAHFKYLDEQIHQLEHELL
SQLHEDERSERLLEIPGIGPMTASVLMSELGDARQYGSARQFAASVGLVPRQYSTGGKPTLLGISKRGDKELRRLLVQCARTIMQRIEHRTDALGVWIRS
LLARRHSNVVACALANKLARIASAILAKGTHYRSIEAVPAV
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase protein is 64, 60 and 41% identical to those of ISBcen1, IS1328 and IS1111 respectively.
The transposase protein is 64, 60 and 41% identical to those of ISBcen1, IS1328 and IS1111 respectively.
References
1] The Welcome Trust Sanger Institute http://www.sanger.ac.uk
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384