ISRle1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Rhizobium leguminosarum | Rhizobium leguminosarum biovar viciae 3841 |
DNA section
IS Length : 1409 bp
Ends
IR Length : 9
IRL : gtgatggATGGCGCCCTCCGACGGCATCGAGTGTGCCAAGATAGGTTGTT
IRR : ----tggATGGCGCCCGTTTGGCAATGTGATTTTGGCCGATGTTTGAGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GTGATGGATGGCGCCCTCCGACGGCATCGAGTGTGCCAAGATAGGTTGTTGCAAAACCTTTGAGGGGAGCCATCCACCATGGAAGAGATTACGACGATTG
GTGTTGATATCGCAAAAAATGTTTTCCAGCTTCACGGCATTAACGCTGAAGGACAGGTCGTTCTGCGCAAGGCCCTGAGACGCGGACAGATGCAACCATT
CTTCGAGGCGCTTTCCCCTTGCCTGATTGGCATGGAAGCCTGCGCGACAGCGCATCACTGGGCGCGAACGCTGATAGCATCTGGCCATGATGTCCGGCTA
ATCCCGCCGAACTACATCAAGCCGTACCTTCGCCGACAGAAGAACGATGCAGCCGATGCTGCAGCCATCTGCGAGGCCGTCACGCGGCCGTCGATGCGGT
TCGTTCCGGTCAAGACCGAGGACCAGCAAGCAACGTTGATGTTGCACAGCGCACGCGAGCTGCTGATCTCGCAACGGACTGCGCTTATCAATGCCTTGCG
TGGTCACTTTGCCGAGCTTGGCATTGTGGCCCCACAGGGCGCTCGCAATGTCCGGCAGTTGATCGCCATTCTGGAAGATGAACGATACAGCCCACAAGCT
GTTCGCACTGCTCTGGCGCCTTTGGCGACAGTGTTGATAGAGATCGAGGGGCAGATCACAAAGCTCGACAAGGCGCTCCTTGCCGCCCATCGCAACAACG
ATGTCAGTATCCGGCTTGCGACCGTTCCCGGAATTGGCCCCGTCATCGCAACTTGCCTTTCGGCCAGCGTCTCAGAGCCAAAGCTGTTCGAGGGCGGTCG
TGAGTTTGCCGCCTGGCTCGGTCTCGTTCCCAGGCAACATTCGACCGGTGGCAAACCAAGGCTGGGTTCCATTTCCAAAATGGGCAACCGGCACCTGCGT
AAACTCCTCGTGGTCGGGGCGTACGCAGCCCTCTACAGGATGAAGAAGGGGACGGCTGATACGCCTCTGGCGAACTGGGCGCGTGGCTTGTTGTCGAAGA
AGCCCTTCAAGCTTGTTGCAGTCGCGCTCGCCAACAAGATTGCCCGCATTGCCTGGGCGATTATGGCGAGGAACGTGAACTTCCAGTCGGAGCACGTTTC
GAGATGCTCCGATCTGATTCCGGCTTAAGCGAAAGGGCCTTCACGGTCATTGCTCTACGGGGTAAACCAGTTGGCGGAGTGACGATGGTATGATGTGACA
GCGAAAGCGGAATGGACCGCACGAACTGAAGAACCCGTGGCATCGTATAGCGCCCCAAAGCGCGGTAAGTTGATAGGGATCGGTTCAAGCGGACAACATC
AGGGCCAGCGGTCATCACGCGCCGCATAAATAGGCCGGAGACATGACTGCACCCGACCCGCAAAGCTCAAACATCGGCCAAAATCACATTGCCAAACGGG
CGCCATCCA
GTGTTGATATCGCAAAAAATGTTTTCCAGCTTCACGGCATTAACGCTGAAGGACAGGTCGTTCTGCGCAAGGCCCTGAGACGCGGACAGATGCAACCATT
CTTCGAGGCGCTTTCCCCTTGCCTGATTGGCATGGAAGCCTGCGCGACAGCGCATCACTGGGCGCGAACGCTGATAGCATCTGGCCATGATGTCCGGCTA
ATCCCGCCGAACTACATCAAGCCGTACCTTCGCCGACAGAAGAACGATGCAGCCGATGCTGCAGCCATCTGCGAGGCCGTCACGCGGCCGTCGATGCGGT
TCGTTCCGGTCAAGACCGAGGACCAGCAAGCAACGTTGATGTTGCACAGCGCACGCGAGCTGCTGATCTCGCAACGGACTGCGCTTATCAATGCCTTGCG
TGGTCACTTTGCCGAGCTTGGCATTGTGGCCCCACAGGGCGCTCGCAATGTCCGGCAGTTGATCGCCATTCTGGAAGATGAACGATACAGCCCACAAGCT
GTTCGCACTGCTCTGGCGCCTTTGGCGACAGTGTTGATAGAGATCGAGGGGCAGATCACAAAGCTCGACAAGGCGCTCCTTGCCGCCCATCGCAACAACG
ATGTCAGTATCCGGCTTGCGACCGTTCCCGGAATTGGCCCCGTCATCGCAACTTGCCTTTCGGCCAGCGTCTCAGAGCCAAAGCTGTTCGAGGGCGGTCG
TGAGTTTGCCGCCTGGCTCGGTCTCGTTCCCAGGCAACATTCGACCGGTGGCAAACCAAGGCTGGGTTCCATTTCCAAAATGGGCAACCGGCACCTGCGT
AAACTCCTCGTGGTCGGGGCGTACGCAGCCCTCTACAGGATGAAGAAGGGGACGGCTGATACGCCTCTGGCGAACTGGGCGCGTGGCTTGTTGTCGAAGA
AGCCCTTCAAGCTTGTTGCAGTCGCGCTCGCCAACAAGATTGCCCGCATTGCCTGGGCGATTATGGCGAGGAACGTGAACTTCCAGTCGGAGCACGTTTC
GAGATGCTCCGATCTGATTCCGGCTTAAGCGAAAGGGCCTTCACGGTCATTGCTCTACGGGGTAAACCAGTTGGCGGAGTGACGATGGTATGATGTGACA
GCGAAAGCGGAATGGACCGCACGAACTGAAGAACCCGTGGCATCGTATAGCGCCCCAAAGCGCGGTAAGTTGATAGGGATCGGTTCAAGCGGACAACATC
AGGGCCAGCGGTCATCACGCGCCGCATAAATAGGCCGGAGACATGACTGCACCCGACCCGCAAAGCTCAAACATCGGCCAAAATCACATTGCCAAACGGG
CGCCATCCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1050 bp | 349 aa | 79 | 1128 | + | No |
Chemistry : DEDD
ORF sequence :
MEEITTIGVDIAKNVFQLHGINAEGQVVLRKALRRGQMQPFFEALSPCLIGMEACATAHHWARTLIASGHDVRLIPPNYIKPYLRRQKNDAADAAAICEA
VTRPSMRFVPVKTEDQQATLMLHSARELLISQRTALINALRGHFAELGIVAPQGARNVRQLIAILEDERYSPQAVRTALAPLATVLIEIEGQITKLDKAL
LAAHRNNDVSIRLATVPGIGPVIATCLSASVSEPKLFEGGREFAAWLGLVPRQHSTGGKPRLGSISKMGNRHLRKLLVVGAYAALYRMKKGTADTPLANW
ARGLLSKKPFKLVAVALANKIARIAWAIMARNVNFQSEHVSRCSDLIPA
VTRPSMRFVPVKTEDQQATLMLHSARELLISQRTALINALRGHFAELGIVAPQGARNVRQLIAILEDERYSPQAVRTALAPLATVLIEIEGQITKLDKAL
LAAHRNNDVSIRLATVPGIGPVIATCLSASVSEPKLFEGGREFAAWLGLVPRQHSTGGKPRLGSISKMGNRHLRKLLVVGAYAALYRMKKGTADTPLANW
ARGLLSKKPFKLVAVALANKIARIAWAIMARNVNFQSEHVSRCSDLIPA
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase protein is 39% identical to that of IS1111.
The transposase protein is 39% identical to that of IS1111.
References
1] The Welcome Trust Sanger Institute http://www.sanger.ac.uk
2] Partridge and Hall (2003) J. Bacteriol. 85, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 85, 6371-6384