ISMlo3
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP003008 | ND | Mesorhizobium loti | Mesorhizobium loti MAFF303099 |
DNA section
IS Length : 1405 bp
Ends
IR Length : 13
IRL : accccatGTGGATGGCTCCCCCACCCGTTGACGGCATTGTGACGGTTGGA
IRR : ----tctGTGGATGGCTCCCGCGGTGCAAGGGGTGATTTGATATTTTGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
ACCCCATGTGGATGGCTCCCCCACCCGTTGACGGCATTGTGACGGTTGGAAGTGGTGCTGTCGCAGCGACCACACGATAGAGGAGCCATCCGCCATGCAA
ATCACCACGATCGGCCTCGATCTGGCCAAGAGCGTATTTCAGTTCTATGCTGTCGACGCTGAAGGACACGTTGTTGTCCGCAAAGCGTTGCGGCGGGCGC
AGATTCTGTCGTTCTTTGCCAAGCTTCCCTGCTGCCTCGTCGGCATGGAAGCTTGCGGCACCTCGCATCATTGGGCGCGCGAGTTGACAAAGCTTGGCCA
CGAGGTGCGGCTGATGCCGCCAGCCTACGTGAAGCCCTATGTGAAGCGGGGCAAGACGGATGCGGGGGATGCCGAGGCGATCTGTGAGGCAGTGACGCGT
CCAACCATGCGCTTCGTGCCGGTGAAGTCGGCCGACCAGCAGGCAGCGCTGGCGCTGCATCGGACGCGCGATCTGCTGGTGAGGCAGCGTACGCAACTTG
TGAACATGCTGCGTGGTCTCTTGGCTGAGTTCGGGATCGAGATAGCACGTGGCCTGCACCATGCATTGCGACTGGCGGGTCGGCTCTCCAACGGCGAAGC
TCCCGAGGTGCCGCCACTGGCTCGGCTGGTGGTGAAGGTATTGGCGGATCAGATCGGCACTATCCAGCTCCAACTCGCCCGTCTGGAGAAGGAGCTCTTG
GCCTGGCACCGCTCCAACGAGTTGTCGCAGCGTTTGGCCACGATCCCGGGCATCGGTGTGATCTCGGCAACCGCACTGGCTGCTTCTGTTATCGAGCCTG
AGCGCTTCCGCTCCGGCCGCCAGTTCGCCGCCTCGCTCGGTCTCACGCCACTCCAGAACTGCTCGGGGGGCCGGGAGCGGATGGGTCGGATCTCGCGAAT
GGGAGACCGCTACCTGCGCCGGCTGTTGGTGGTCGGCATGACCTCGCTCGTCCGACGGGCCAGGATACAGCCGGCATCGGTTGATCCGCGCCTATCTGCC
ATGCTGGCGCGCAAACCGGCGCGTCTCGTCACGGTGGCTGCCGCCAACAGGACGGCGCGCGTGGCCTGGGCGATCATGACGCGCGGAGGAACCTTCAGGG
CGCCGATCAGCGCCGCAGCCTGATCTGCTTCGGCGCCAAGCAAACAGATACGAAGAAGCTGGAAGTTTACCAGTTGCAAGGGCGAAGAGATGTGATGGCG
AACCGGTCATATCGGGAGTCGGGACAACCCGTGCGAATGTCGTGGGCGTCATTGCCCGCAAAGCAGAGTGGGACCTGACTCGCGGATACCATCAAGGCCA
GCGGTCAACATGAACGACTGCATCAACAGGCCGGACAGAAGACTGCACCTGACCCAATCGTCAAAATATCAAATCACCCCTTGCACCGCGGGAGCCATCC
ACAGA
ATCACCACGATCGGCCTCGATCTGGCCAAGAGCGTATTTCAGTTCTATGCTGTCGACGCTGAAGGACACGTTGTTGTCCGCAAAGCGTTGCGGCGGGCGC
AGATTCTGTCGTTCTTTGCCAAGCTTCCCTGCTGCCTCGTCGGCATGGAAGCTTGCGGCACCTCGCATCATTGGGCGCGCGAGTTGACAAAGCTTGGCCA
CGAGGTGCGGCTGATGCCGCCAGCCTACGTGAAGCCCTATGTGAAGCGGGGCAAGACGGATGCGGGGGATGCCGAGGCGATCTGTGAGGCAGTGACGCGT
CCAACCATGCGCTTCGTGCCGGTGAAGTCGGCCGACCAGCAGGCAGCGCTGGCGCTGCATCGGACGCGCGATCTGCTGGTGAGGCAGCGTACGCAACTTG
TGAACATGCTGCGTGGTCTCTTGGCTGAGTTCGGGATCGAGATAGCACGTGGCCTGCACCATGCATTGCGACTGGCGGGTCGGCTCTCCAACGGCGAAGC
TCCCGAGGTGCCGCCACTGGCTCGGCTGGTGGTGAAGGTATTGGCGGATCAGATCGGCACTATCCAGCTCCAACTCGCCCGTCTGGAGAAGGAGCTCTTG
GCCTGGCACCGCTCCAACGAGTTGTCGCAGCGTTTGGCCACGATCCCGGGCATCGGTGTGATCTCGGCAACCGCACTGGCTGCTTCTGTTATCGAGCCTG
AGCGCTTCCGCTCCGGCCGCCAGTTCGCCGCCTCGCTCGGTCTCACGCCACTCCAGAACTGCTCGGGGGGCCGGGAGCGGATGGGTCGGATCTCGCGAAT
GGGAGACCGCTACCTGCGCCGGCTGTTGGTGGTCGGCATGACCTCGCTCGTCCGACGGGCCAGGATACAGCCGGCATCGGTTGATCCGCGCCTATCTGCC
ATGCTGGCGCGCAAACCGGCGCGTCTCGTCACGGTGGCTGCCGCCAACAGGACGGCGCGCGTGGCCTGGGCGATCATGACGCGCGGAGGAACCTTCAGGG
CGCCGATCAGCGCCGCAGCCTGATCTGCTTCGGCGCCAAGCAAACAGATACGAAGAAGCTGGAAGTTTACCAGTTGCAAGGGCGAAGAGATGTGATGGCG
AACCGGTCATATCGGGAGTCGGGACAACCCGTGCGAATGTCGTGGGCGTCATTGCCCGCAAAGCAGAGTGGGACCTGACTCGCGGATACCATCAAGGCCA
GCGGTCAACATGAACGACTGCATCAACAGGCCGGACAGAAGACTGCACCTGACCCAATCGTCAAAATATCAAATCACCCCTTGCACCGCGGGAGCCATCC
ACAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 95 | 1123 | + | No |
Chemistry : DEDD
ORF sequence :
MQITTIGLDLAKSVFQFYAVDAEGHVVVRKALRRAQILSFFAKLPCCLVGMEACGTSHHWARELTKLGHEVRLMPPAYVKPYVKRGKTDAGDAEAICEAV
TRPTMRFVPVKSADQQAALALHRTRDLLVRQRTQLVNMLRGLLAEFGIEIARGLHHALRLAGRLSNGEAPEVPPLARLVVKVLADQIGTIQLQLARLEKE
LLAWHRSNELSQRLATIPGIGVISATALAASVIEPERFRSGRQFAASLGLTPLQNCSGGRERMGRISRMGDRYLRRLLVVGMTSLVRRARIQPASVDPRL
SAMLARKPARLVTVAAANRTARVAWAIMTRGGTFRAPISAAA
TRPTMRFVPVKSADQQAALALHRTRDLLVRQRTQLVNMLRGLLAEFGIEIARGLHHALRLAGRLSNGEAPEVPPLARLVVKVLADQIGTIQLQLARLEKE
LLAWHRSNELSQRLATIPGIGVISATALAASVIEPERFRSGRQFAASLGLTPLQNCSGGRERMGRISRMGDRYLRRLLVVGMTSLVRRARIQPASVDPRL
SAMLARKPARLVTVAAANRTARVAWAIMTRGGTFRAPISAAA
Blast result :
Comments
No uninterrupted target sequence was found, so the ends of the IS have been defined by analogy with other IS1111 family elements, assuming that 7 nt separate IRl from the left-hand end of the element and 4 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right..
The transposase gene corresponds to orf mlr5983 and the transposase protein is 82% identical to that of ISMmg2 and 37% identical to that of IS1111.
The transposase gene corresponds to orf mlr5983 and the transposase protein is 82% identical to that of ISMmg2 and 37% identical to that of IS1111.
References
1] Kaneko et al. (2000) DNA Res. 7, 331-338
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384