ISShsp1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB091693 | ND | Sphingomonas sp. | Sphingomonas sp. P2 |
DNA section
IS Length : 1404 bp
Ends
IR Length : 13
IRL : tgttgggGTGGACGGCTCCCAACGGCATCGCAATGTGCCAGAGTGAGTGC
IRR : ----tatGTGGACGGCTCCCTCTTGCAAGGGCTGAACGGAAAATTTGATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGTTGGGGTGGACGGCTCCCAACGGCATCGCAATGTGCCAGAGTGAGTGCGTTGCAAGACTCAATCAAGGGAGCCGCCCGTGGAGCAAATTACCCGCATA
GGCATGGATACGTCAAAGAACGTTTTTCAACTGCATGGCGTCGATGCAGCGGAGAAGGCAGTGCTGCGCAAGAAGTTGACGCGCCGGGAAATGGTTCGGT
TTTTCGAAAAGCTGCCACCCACCGTCGTCGCTATCGAGGCATGCGGCAGTTCACATCATTGGGCTCGTCTTCTCGGATCGCTCGGGCATGACGTAAGGTT
GATCGCGCCGCAATTAGCGAAGCCCTACGTCAAGCGCGGCAAGAACGACGCGGCGGATGCCGAAGCTCTCTGCGAAGCGGTGAGCCGCCCAACAATGCGC
TTCGTTCCAGTAAAGACTGCAGAACAACAGGCGGCGCTCATGTTGGTCGGGGTGCGCGAAAGGCTCATCCAGAAGCGCACCCAGCTCGTCAACGCTATAC
GAGGGTATGGCGCGGAGTTCGGCATCACTGCGGCGACGGGTGCATGTCGCGTCGTACCGCTTCTCGAGCGCATCGCGGCAGATGAAACGATACCCGAATT
GGCGCGCGAACTTTTTGCCCTGCAGGGAAAGGAATATGCGCAGCTCCAGTGCGAGATCGAACGGATCGACGCGAAGCTGATGGAATGGCATCGCAACGAC
GAATGTAGTCAGCGACTGGCCAAAATTCCCGGCGTTGGGCCTATCGGCGCGACCTTGCTCATGATGAAAACGCCCGATCCGCATCTATTCAAATCCGGTC
GGGATTTTGCGGCATGGATGGGCCTGACCCCTAAAGATCACTCGACCGCCGGCAAGACAAGACACGGCGTTATCACTCGCGCTGGTGATGAGCTTCTCAG
AAGCACGCTCGTGGTCGGAGCTACCGCAGTCGTTCAGCACGCCCGCAATGGGCGAGGTAGGAATCTCACACCATGGCTTATCGGCTTGATTGGACGAAAA
TCACCTAAGCTCGTTGCGGTTGCCGTTGCTAACAAAGCCGCACGTGTCGCGTGGAAACTCATGGTGAGCGGCGAGCGATACCAATGCGTCGCGCCGCCCG
CCCTTACTGCGGTAGCAGCATAAGAATTAGCCAGGAGCGATGAGCAGAATATCAATGCTATCGTACTGAGCTGATCCGTGATCTTGCATGAAGAGAGCAG
ATGGTGCGATCGATCGATCCGAGGCGTGTGACACTCCGTCAGTTCCAGTGGCCATCACAGGCCGCTGCCGTGTTGGGAACACACACCGCGGAAACCATCT
TGGCCAGCGGTCACGTGCGACCGCACCGAAAGGCCGGACATATGGAAGCAAGCGATCCGATCAAATTTTCCGTTCAGCCCTTGCAAGAGGGAGCCGTCCA
CATA
GGCATGGATACGTCAAAGAACGTTTTTCAACTGCATGGCGTCGATGCAGCGGAGAAGGCAGTGCTGCGCAAGAAGTTGACGCGCCGGGAAATGGTTCGGT
TTTTCGAAAAGCTGCCACCCACCGTCGTCGCTATCGAGGCATGCGGCAGTTCACATCATTGGGCTCGTCTTCTCGGATCGCTCGGGCATGACGTAAGGTT
GATCGCGCCGCAATTAGCGAAGCCCTACGTCAAGCGCGGCAAGAACGACGCGGCGGATGCCGAAGCTCTCTGCGAAGCGGTGAGCCGCCCAACAATGCGC
TTCGTTCCAGTAAAGACTGCAGAACAACAGGCGGCGCTCATGTTGGTCGGGGTGCGCGAAAGGCTCATCCAGAAGCGCACCCAGCTCGTCAACGCTATAC
GAGGGTATGGCGCGGAGTTCGGCATCACTGCGGCGACGGGTGCATGTCGCGTCGTACCGCTTCTCGAGCGCATCGCGGCAGATGAAACGATACCCGAATT
GGCGCGCGAACTTTTTGCCCTGCAGGGAAAGGAATATGCGCAGCTCCAGTGCGAGATCGAACGGATCGACGCGAAGCTGATGGAATGGCATCGCAACGAC
GAATGTAGTCAGCGACTGGCCAAAATTCCCGGCGTTGGGCCTATCGGCGCGACCTTGCTCATGATGAAAACGCCCGATCCGCATCTATTCAAATCCGGTC
GGGATTTTGCGGCATGGATGGGCCTGACCCCTAAAGATCACTCGACCGCCGGCAAGACAAGACACGGCGTTATCACTCGCGCTGGTGATGAGCTTCTCAG
AAGCACGCTCGTGGTCGGAGCTACCGCAGTCGTTCAGCACGCCCGCAATGGGCGAGGTAGGAATCTCACACCATGGCTTATCGGCTTGATTGGACGAAAA
TCACCTAAGCTCGTTGCGGTTGCCGTTGCTAACAAAGCCGCACGTGTCGCGTGGAAACTCATGGTGAGCGGCGAGCGATACCAATGCGTCGCGCCGCCCG
CCCTTACTGCGGTAGCAGCATAAGAATTAGCCAGGAGCGATGAGCAGAATATCAATGCTATCGTACTGAGCTGATCCGTGATCTTGCATGAAGAGAGCAG
ATGGTGCGATCGATCGATCCGAGGCGTGTGACACTCCGTCAGTTCCAGTGGCCATCACAGGCCGCTGCCGTGTTGGGAACACACACCGCGGAAACCATCT
TGGCCAGCGGTCACGTGCGACCGCACCGAAAGGCCGGACATATGGAAGCAAGCGATCCGATCAAATTTTCCGTTCAGCCCTTGCAAGAGGGAGCCGTCCA
CATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1044 bp | 347 aa | 80 | 1123 | + | No |
Chemistry : DEDD
ORF sequence :
MEQITRIGMDTSKNVFQLHGVDAAEKAVLRKKLTRREMVRFFEKLPPTVVAIEACGSSHHWARLLGSLGHDVRLIAPQLAKPYVKRGKNDAADAEALCEA
VSRPTMRFVPVKTAEQQAALMLVGVRERLIQKRTQLVNAIRGYGAEFGITAATGACRVVPLLERIAADETIPELARELFALQGKEYAQLQCEIERIDAKL
MEWHRNDECSQRLAKIPGVGPIGATLLMMKTPDPHLFKSGRDFAAWMGLTPKDHSTAGKTRHGVITRAGDELLRSTLVVGATAVVQHARNGRGRNLTPWL
IGLIGRKSPKLVAVAVANKAARVAWKLMVSGERYQCVAPPALTAVAA
VSRPTMRFVPVKTAEQQAALMLVGVRERLIQKRTQLVNAIRGYGAEFGITAATGACRVVPLLERIAADETIPELARELFALQGKEYAQLQCEIERIDAKL
MEWHRNDECSQRLAKIPGVGPIGATLLMMKTPDPHLFKSGRDFAAWMGLTPKDHSTAGKTRHGVITRAGDELLRSTLVVGATAVVQHARNGRGRNLTPWL
IGLIGRKSPKLVAVAVANKAARVAWKLMVSGERYQCVAPPALTAVAA
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase gene corresponds to orf26 and the transposase protein is 74, 73, 71 and 38% identical to those of ISRsp4, ISMlo2, ISMlo1 and IS11111, respectively.
By analogy with IS4321, ISShsp1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
The transposase gene corresponds to orf26 and the transposase protein is 74, 73, 71 and 38% identical to those of ISRsp4, ISMlo2, ISMlo1 and IS11111, respectively.
By analogy with IS4321, ISShsp1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Pinyakong et al. (2003) Biochem. Biophys. Res. Commun. 301, 350-357
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384