ISSfl8

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF326777 ND Shigella flexneri
Shigella flexneri 2a
DNA section
IS Length : 1357 bp

Ends


IR Length : 12

IRL : taatgatATGGTCACCCCCACCTTAACAGCAGTACGCTGTTAAGGTAAAT
IRR : ----tctATGGTCACCCCCGTTTTTGCAACACTGATTTTTGATTAATGGT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TAATGATATGGTCACCCCCACCTTAACAGCAGTACGCTGTTAAGGTAAATCCCTTTAACGGAGAGACCATCATGCAGAACATCACACTTATTGGTATCGA
TCTCGGAAAGCACTCCTTCCACATTCATTGTCAAGACAAGTCTGGTAAGGCTCTTCTGCGTAAAAAATTTACCCGTACTAAACTGATGCAGTTTCTGGCG
ACATGTCCGCCCTCAGTTGTTGTAATGGAAGCCTGTGCGGGTGCACACTTTATGGCTCGCTGTATCTCTGATTTTGGGCATGAAACTAAGCTGATTTCTC
CACAGTTCGTTCGCCCGTTTGTCAAAAGTAATAAGAATGATTTTGTTGATGCAGAAGCCATATGCGAAGCGGCTTCACGACCATCAATGCGTTTTGTCCA
GCCAAGAACTGAGACCCAGCAGGCCATGAGGGCGTTACACCGGGTCAGAGAATCGCTCATCAGAGATAAGGTGAAAACAACCAACCAGATCCACGGTTTC
TTGCTGGAATTTGGAATCAGCCTGCCGATAGGTGATGCTGTAATAAAACGGTTGTCTCTCGTGTTGGCTGAACACGAAATCCCCGAATATCTGAGCCGCC
TTTTGGTGAGGTTGCATACCCACTACTTTTACCTTGTTGAGCAAATAGCAGAGCTGGAATCAGAATTAAGTCAGTCAATCAACGCTGATGATACCGCTCA
GCGCATTATGACAATTCCTGGTGTTGGTCCGATTACTGCCAGCTTACTATCGTCGCAACTTGGTGACGGGAAACAGTTCTCATGCAGCAGGGATTTTGCA
GCTTCCACAGGACTTGTTCCCCGGCAGTATAGCACTGGAGGAAAATCGACTCTGTTGGGTATCAGTAAGCGAGGTGATAAAAATTTACGCCGGTTACTGG
TTCAGTGTGCCCGCTCATTCATAATGCGACTTGAACGCAAGCATGGAAAACTTGCTGAGTGGGTTCGGGAGCAACTCAACAAGAAGCATTCGAATGTGGT
TACCTGCGCTCTGGCAAATAAACTGGCTCGAATTGCCTGGGCAATCACTACTCAGAAAAAAGAATATCAGGCCTGATATAGATGCTGATATAACTCAATT
AGTTTATTTTAAACAATAAGATACAAGTTACCTTTCTGGTTTTGCGAAGACTGATTATTGATGACATGAACGGCAAATCGACCTGATTAAAAACCTAACA
GATGAAATGGCTCACTGAAGCCGAGGGGATTTTTAGGATTATCAGGTGCGGAACTCATCGGGGCGCGGGCATCCTTGCCCACAGAGACGCCGGATAGATT
TAAGCAAGCCAACCATTAATCAAAAATCAGTGTTGCAAAAACGGGGGTGACCATAGA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1005 bp334 aa721076+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLATCPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEA
ASRPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELS
QSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLLVQCARSFIMRLERKHGKLAEWVRE
QLNKKHSNVVTCALANKLARIAWAITTQKKEYQA

 

Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.

The transposase protein is 73, 72, 70 and 40% to those of ISEch4, IS1328, IS4321L and IS1111, respectively.

By analogy with IS4321, ISSfl8 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Luck et al. (2001) Infect. Immun. 69, 6012-6021
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384