ISSod1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004347 | ND | Shewanella oneidensis | Shewanella oneidensis MR-1 |
DNA section
IS Length : 1235 bp
Ends
IR Length : 26/38
IRL : TGTTCTGGTCTAATCCCATCGGACACTTAATTTTGAGACAGTATGGTCAA
IRR : TGTACTGGTCAACCCAAACTGGACACTTTTACTTGAGAATTCTCAAACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGTTCTGGTCTAATCCCATCGGACACTTAATTTTGAGACAGTATGGTCAATAAATTAAGAGGTCTATATGAGTCTGAAAAAATCACATAAGAGTTATCCG
CAGGCATTTAAAGATGAAGCCGTCTTGATGGTGCTGGAGCAAGGTTATAGCGTTGCCGATGCGGCAAAGTCTCTTGGAGTTAGCACGAGCCTGCTTTACA
ACTGGAAGGAAAAACACGAAGCCCTGCAACAAGGCATCACCTTAGAAGAGTCTGAGCGTGATGAGTTGAAGCGATTGCGTAGAGAAAACAAAGAATTACG
CATGGAGAAAGAAATTCTAAAAAAGGCAAGCGCCTTCTTTGCGAGAGAAATGAAGTAAGATTTCGTTTCATCAAACTGCAATCTCACCTGTTTCCCATAA
CACTGTTATGTCGAGTAATGAGTGTCAGTAAGTCAGGCTATTACGATTGGCATAAACGCCCTGCAAACGTGATAAGCGTTGAAACACTGAAGCTTTATCG
CCTTGTTCGACAGCTATTTAAGCAAAGTCGAGGCAGCTTAGGGAATCGTGAAATGGTGAAGAAATTGCGCAAGGAAGGCTACCAGGTTGGTCGCTATCTC
GTTCGTAAAATTATGCACCGCCTTCGACTCAAAGCAACCCAGCGATGTGCTTACAAGGTGACGACACAGCGAAAACACTCAGATGCAGTGGCTGATAACC
TGTTAAACATGAACTTTAATCCAGTATCGGCTAATCAGGTCTGGGCGGGTGACGTGACCTATTTAAAGACGGGTGAAGGCTGGATGTACTTAGCTGTGGT
GATGGATTTATATTCACGCCGGATTGTGGGATGGCGCATAGACAAACGCATGACCACAGATTTGATATCCAAGGCATTAATAAAAGCCTACAACCTGCGA
CAACCAGCGCGAGGGCTGGTATTTCACAGTGACCGAGGCTCGCAATATACCAGTAAACAATTCGGTAGGCTGCTATCGAGCTATGGTATCCGAGCCAGCA
TGGGTGATGTGGGTGCGTGTTGGGATAATGCCGTTGTTGAGCGATTCTTTGGTAGCTTGAAACACGATTGGATTTTTAAAGTTGCTCAACCAACAAGGGA
GTTTATGAAGCAAGATGTGACGGCTTACATCAAATATTACAACTTGGAGCGACTTCATTCTGCTAATAACGATCTGTCACCTGTAGAGTTTGAGAATTCT
CAAGTAAAAGTGTCCAGTTTGGGTTGACCAGTACA
CAGGCATTTAAAGATGAAGCCGTCTTGATGGTGCTGGAGCAAGGTTATAGCGTTGCCGATGCGGCAAAGTCTCTTGGAGTTAGCACGAGCCTGCTTTACA
ACTGGAAGGAAAAACACGAAGCCCTGCAACAAGGCATCACCTTAGAAGAGTCTGAGCGTGATGAGTTGAAGCGATTGCGTAGAGAAAACAAAGAATTACG
CATGGAGAAAGAAATTCTAAAAAAGGCAAGCGCCTTCTTTGCGAGAGAAATGAAGTAAGATTTCGTTTCATCAAACTGCAATCTCACCTGTTTCCCATAA
CACTGTTATGTCGAGTAATGAGTGTCAGTAAGTCAGGCTATTACGATTGGCATAAACGCCCTGCAAACGTGATAAGCGTTGAAACACTGAAGCTTTATCG
CCTTGTTCGACAGCTATTTAAGCAAAGTCGAGGCAGCTTAGGGAATCGTGAAATGGTGAAGAAATTGCGCAAGGAAGGCTACCAGGTTGGTCGCTATCTC
GTTCGTAAAATTATGCACCGCCTTCGACTCAAAGCAACCCAGCGATGTGCTTACAAGGTGACGACACAGCGAAAACACTCAGATGCAGTGGCTGATAACC
TGTTAAACATGAACTTTAATCCAGTATCGGCTAATCAGGTCTGGGCGGGTGACGTGACCTATTTAAAGACGGGTGAAGGCTGGATGTACTTAGCTGTGGT
GATGGATTTATATTCACGCCGGATTGTGGGATGGCGCATAGACAAACGCATGACCACAGATTTGATATCCAAGGCATTAATAAAAGCCTACAACCTGCGA
CAACCAGCGCGAGGGCTGGTATTTCACAGTGACCGAGGCTCGCAATATACCAGTAAACAATTCGGTAGGCTGCTATCGAGCTATGGTATCCGAGCCAGCA
TGGGTGATGTGGGTGCGTGTTGGGATAATGCCGTTGTTGAGCGATTCTTTGGTAGCTTGAAACACGATTGGATTTTTAAAGTTGCTCAACCAACAAGGGA
GTTTATGAAGCAAGATGTGACGGCTTACATCAAATATTACAACTTGGAGCGACTTCATTCTGCTAATAACGATCTGTCACCTGTAGAGTTTGAGAATTCT
CAAGTAAAAGTGTCCAGTTTGGGTTGACCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 68 | 358 | + | No |
Description : First part of the transposase
ORF sequence :
MSLKKSHKSYPQAFKDEAVLMVLEQGYSVADAAKSLGVSTSLLYNWKEKHEALQQGITLEESERDELKRLRRENKELRMEKEILKKASAFFAREMK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 283 | 1227 | + | No |
Description : Second part of the transposase
ORF sequence :
RKQRITHGERNSKKGKRLLCERNEVRFRFIKLQSHLFPITLLCRVMSVSKSGYYDWHKRPANVISVETLKLYRLVRQLFKQSRGSLGNREMVKKLRKEGY
QVGRYLVRKIMHRLRLKATQRCAYKVTTQRKHSDAVADNLLNMNFNPVSANQVWAGDVTYLKTGEGWMYLAVVMDLYSRRIVGWRIDKRMTTDLISKALI
KAYNLRQPARGLVFHSDRGSQYTSKQFGRLLSSYGIRASMGDVGACWDNAVVERFFGSLKHDWIFKVAQPTREFMKQDVTAYIKYYNLERLHSANNDLSP
VEFENSQVKVSSLG
QVGRYLVRKIMHRLRLKATQRCAYKVTTQRKHSDAVADNLLNMNFNPVSANQVWAGDVTYLKTGEGWMYLAVVMDLYSRRIVGWRIDKRMTTDLISKALI
KAYNLRQPARGLVFHSDRGSQYTSKQFGRLLSSYGIRASMGDVGACWDNAVVERFFGSLKHDWIFKVAQPTREFMKQDVTAYIKYYNLERLHSANNDLSP
VEFENSQVKVSSLG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 68 | 1227 | + | Yes |
Chemistry : DDE
ORF sequence :
MSLKKSHKSYPQAFKDEAVLMVLEQGYSVADAAKSLGVSTSLLYNWKEKHEALQQGITLEESERDELKRLRRENKELRMEKEILKKGKRLLCERNEVRFR
FIKLQSHLFPITLLCRVMSVSKSGYYDWHKRPANVISVETLKLYRLVRQLFKQSRGSLGNREMVKKLRKEGYQVGRYLVRKIMHRLRLKATQRCAYKVTT
QRKHSDAVADNLLNMNFNPVSANQVWAGDVTYLKTGEGWMYLAVVMDLYSRRIVGWRIDKRMTTDLISKALIKAYNLRQPARGLVFHSDRGSQYTSKQFG
RLLSSYGIRASMGDVGACWDNAVVERFFGSLKHDWIFKVAQPTREFMKQDVTAYIKYYNLERLHSANNDLSPVEFENSQVKVSSLG
FIKLQSHLFPITLLCRVMSVSKSGYYDWHKRPANVISVETLKLYRLVRQLFKQSRGSLGNREMVKKLRKEGYQVGRYLVRKIMHRLRLKATQRCAYKVTT
QRKHSDAVADNLLNMNFNPVSANQVWAGDVTYLKTGEGWMYLAVVMDLYSRRIVGWRIDKRMTTDLISKALIKAYNLRQPARGLVFHSDRGSQYTSKQFG
RLLSSYGIRASMGDVGACWDNAVVERFFGSLKHDWIFKVAQPTREFMKQDVTAYIKYYNLERLHSANNDLSPVEFENSQVKVSSLG
Blast result :
Comments
There are 38 chromosomal insertions and 9 plasmid insertions of ISSod1 in this strain.
ISSod1 is 75% aa similar to ISPin3.
ISSod1 is 75% aa similar to ISPin3.
References