ISGsu7

  • Family IS3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE017180 ND Geobacter sulfurreducens
Geobacter sulfurreducens PCA
DNA section
IS Length : 1351 bp

Ends


IR Length : 14/22

IRL : TATGGCGGCCCCGGTTTTCTTGGACACGAAAATGAGGTAAATCTAGGTGT
IRR : TGTAGCGCGCCCGGTACCCTGGACACAAGATAAGAGTCACGCAGCCTTCT

Insertion site


Left flankDirect repeatRight flankDR Length
TCTGAGCATAGAAATAACCAAGGGGGAAATGGAC14
TGCAAATTAGGAAAATCAATCTTTCCAGGGGATT14
AAGAAGGAAAGCAGGATGAAAATGCCGAACAGTA14
GGAGCCAGAAGATTCGTGAAGAAATCAACCTGCT14
ATTCAGCTGTAATAACCGTAATATCTGCTGCATC14
TCAGAATGACCAATCTGTTCGAGGAGGCGGTCCT14
AAACCCCAAAGAATTTACGTACGACTCCAATGCA14
CTCCTCCAGACTGCTGATATCTCCCTCACGGGCA14

DNA sequence

TATGGCGGCCCCGGTTTTCTTGGACACGAAAATGAGGTAAATCTAGGTGTTCAAGGAGGTCGTCAATGGAAAAGGATGTCAGTGTCTCCGGTGCGGCGGA
AGGAGAGCGTAGCTCGACTGGAGCAGCACCGGAAGTCAAACGTTGGAGCGCCAACCGCAAAAAGGAGGTTGTCCTGCGGTTGATGCGCGGTGAACCAATC
GATGCCTTGTCCCGTGAGTTGGGCATCGAAATCTATCGCCTGGAAGAGTGGCATCAGAAAGCACTCCAGGGGATAGAATCTGCTCTCAAGGCTCGTGAAG
GTGATCCGTTGGCAGCGGAACTCGATGCAGCCTTCAAGCGCATCGGTGAGATCACCATGGAAAACGAGCTACTGCGGGAGAAAGCGAGGCGTGCCCACCC
TTTGGCCCAGAGGAGATCGAAGAAATGAGTGCTACGATCTCCCCTGCCACCGGTAAAGCCTATGGCGTTCAGCGCGTCTGCCTTGCCTGGGGAGTGCCCC
GCTCGTCGTTCTATAGCCGTGCAGGCAGAAAAACATTGCCTGCTGTACTGCTCAAGCGTGGTCCAAAAACACCGCTCTCTGACGACGAGGTACTTTCTCT
CATCCGGACCGACCTGGAGACATCCCCCTTTGTCGGCGAAGGGCACCGTAAGGTCTGGGGACGACTACGCTTCGTCAAAGGGATAAAAGTCGGCCGCAAA
CGAGTGTTGCGTCTCATGCGGGAGAACAATCTGCTCTCCCCTCACCGGGTTGTTCAGGGACAGCCCAAGGACCACGCCGGCAAGATCATCACGGCGGCCC
CCAACGTCATGTGGGGTACCGATGGCACCAAGATCTTCACCCTGGAAGAAGGGTGGTGCTGGTTGTTTACCGCCGTCGAGCATTGGAATGCCGAGTGCGT
CGGTTGGCATGTGACCAAGAATGGCGACCGGTTTGCCGCATTGCAGCCGCTCGCCATGGGAATCAAGGCCCGTTTCGGCTCGGTCGGTGCCGCTGCTGCT
CGAGGGTTGGCACTGAGGATGGATCACGGCAGCCAATACCTCTCAGAGCATTTCCAGAATCAGATCAAGTTCTGGGGAATTGCCCCAAGTTTCTCATTTG
TTGCCGAACCGCAGACCAACGGGGTGACGGAACGGTTTAATCGGACTATCAAAGAGCAGGTCATCTATGGCCGCCATTACCGCACAATCGAAGAAGTAAG
GATGGCAGTAGCTGACTTCATGGATCGTTACAACCGGCTGTGGCTGGTTGAAAAGCTCGGGTTCAGAAGCCCACGACAGGCTTTCGAGGAGTATCAACTC
AAGAAGGCTGCGTGACTCTTATCTTGTGTCCAGGGTACCGGGCGCGCTACA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : Yes
  • Secondary structure : stem-loop

Recoding motif :

GAGGCGTGCCCACCCTTTGGCCCAGAGGAGATCGAAGAAATGAGTGCTAC
--------------------------((((((((-((---------))-)
GATCTCC
)))))))
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
363 bp120 aa66428+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MEKDVSVSGAAEGERSSTGAAPEVKRWSANRKKEVVLRLMRGEPIDALSRELGIEIYRLEEWHQKALQGIESALKAREGDPLAAELDAAFKRIGEITMEN
ELLREKARRAHPLAQRRSKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
966 bp296 aa3501315+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DHHGKRATAGESEACPPFGPEEIEMSATISPATGKAYGVQRVCLAWGVPRSSFYSRAGRKTLPAVLLKRGPKTPLSDDEVLSLIRTDLETSPFVGEGHRK
VWGRLRFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPL
AMGIKARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVE
KLGFRSPRQAFEEYQLKKAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1250 bp415 aa661315+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEKDVSVSGAAEGERSSTGAAPEVKRWSANRKKEVVLRLMRGEPIDALSRELGIEIYRLEEWHQKALQGIESALKAREGDPLAAELDAAFKRIGEITMEN
ELLREKARRAHPFGPEEIEMSATISPATGKAYGVQRVCLAWGVPRSSFYSRAGRKTLPAVLLKRGPKTPLSDDEVLSLIRTDLETSPFVGEGHRKVWGRL
RFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPLAMGIK
ARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVEKLGFR
SPRQAFEEYQLKKAA

 

Blast result :
Comments
Two of the ten insertions in this strain lack a direct repeat.
ISGsu7 is 61% aa similar to ISMtsp5.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
References
1] Methe BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, Dodson RJ, Madupu R, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Gwinn M, Kolonay JF, Sullivan SA, Haft DH, Selengut J, Davidsen TM, Zafar N, White O, Tran B, Romero C, Forberger HA, Weidman J, Khouri H, Feldblyum TV, Utterback TR, Van Aken SE, Lovley DR, Fraser CM. (2003) Science 302 (5652), 1967-1969