ISGsu7
- Family IS3
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AE017180 | ND | Geobacter sulfurreducens | Geobacter sulfurreducens PCA |
DNA section
IS Length : 1351 bp
Ends
IR Length : 14/22
IRL : TATGGCGGCCCCGGTTTTCTTGGACACGAAAATGAGGTAAATCTAGGTGT
IRR : TGTAGCGCGCCCGGTACCCTGGACACAAGATAAGAGTCACGCAGCCTTCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TCTGAGCATA | GAAATAACCAAGGG | GGAAATGGAC | 14 |
TGCAAATTAG | GAAAATCAATCTTT | CCAGGGGATT | 14 |
AAGAAGGAAA | GCAGGATGAAAATG | CCGAACAGTA | 14 |
GGAGCCAGAA | GATTCGTGAAGAAA | TCAACCTGCT | 14 |
ATTCAGCTGT | AATAACCGTAATAT | CTGCTGCATC | 14 |
TCAGAATGAC | CAATCTGTTCGAGG | AGGCGGTCCT | 14 |
AAACCCCAAA | GAATTTACGTACGA | CTCCAATGCA | 14 |
CTCCTCCAGA | CTGCTGATATCTCC | CTCACGGGCA | 14 |
DNA sequence
TATGGCGGCCCCGGTTTTCTTGGACACGAAAATGAGGTAAATCTAGGTGTTCAAGGAGGTCGTCAATGGAAAAGGATGTCAGTGTCTCCGGTGCGGCGGA
AGGAGAGCGTAGCTCGACTGGAGCAGCACCGGAAGTCAAACGTTGGAGCGCCAACCGCAAAAAGGAGGTTGTCCTGCGGTTGATGCGCGGTGAACCAATC
GATGCCTTGTCCCGTGAGTTGGGCATCGAAATCTATCGCCTGGAAGAGTGGCATCAGAAAGCACTCCAGGGGATAGAATCTGCTCTCAAGGCTCGTGAAG
GTGATCCGTTGGCAGCGGAACTCGATGCAGCCTTCAAGCGCATCGGTGAGATCACCATGGAAAACGAGCTACTGCGGGAGAAAGCGAGGCGTGCCCACCC
TTTGGCCCAGAGGAGATCGAAGAAATGAGTGCTACGATCTCCCCTGCCACCGGTAAAGCCTATGGCGTTCAGCGCGTCTGCCTTGCCTGGGGAGTGCCCC
GCTCGTCGTTCTATAGCCGTGCAGGCAGAAAAACATTGCCTGCTGTACTGCTCAAGCGTGGTCCAAAAACACCGCTCTCTGACGACGAGGTACTTTCTCT
CATCCGGACCGACCTGGAGACATCCCCCTTTGTCGGCGAAGGGCACCGTAAGGTCTGGGGACGACTACGCTTCGTCAAAGGGATAAAAGTCGGCCGCAAA
CGAGTGTTGCGTCTCATGCGGGAGAACAATCTGCTCTCCCCTCACCGGGTTGTTCAGGGACAGCCCAAGGACCACGCCGGCAAGATCATCACGGCGGCCC
CCAACGTCATGTGGGGTACCGATGGCACCAAGATCTTCACCCTGGAAGAAGGGTGGTGCTGGTTGTTTACCGCCGTCGAGCATTGGAATGCCGAGTGCGT
CGGTTGGCATGTGACCAAGAATGGCGACCGGTTTGCCGCATTGCAGCCGCTCGCCATGGGAATCAAGGCCCGTTTCGGCTCGGTCGGTGCCGCTGCTGCT
CGAGGGTTGGCACTGAGGATGGATCACGGCAGCCAATACCTCTCAGAGCATTTCCAGAATCAGATCAAGTTCTGGGGAATTGCCCCAAGTTTCTCATTTG
TTGCCGAACCGCAGACCAACGGGGTGACGGAACGGTTTAATCGGACTATCAAAGAGCAGGTCATCTATGGCCGCCATTACCGCACAATCGAAGAAGTAAG
GATGGCAGTAGCTGACTTCATGGATCGTTACAACCGGCTGTGGCTGGTTGAAAAGCTCGGGTTCAGAAGCCCACGACAGGCTTTCGAGGAGTATCAACTC
AAGAAGGCTGCGTGACTCTTATCTTGTGTCCAGGGTACCGGGCGCGCTACA
AGGAGAGCGTAGCTCGACTGGAGCAGCACCGGAAGTCAAACGTTGGAGCGCCAACCGCAAAAAGGAGGTTGTCCTGCGGTTGATGCGCGGTGAACCAATC
GATGCCTTGTCCCGTGAGTTGGGCATCGAAATCTATCGCCTGGAAGAGTGGCATCAGAAAGCACTCCAGGGGATAGAATCTGCTCTCAAGGCTCGTGAAG
GTGATCCGTTGGCAGCGGAACTCGATGCAGCCTTCAAGCGCATCGGTGAGATCACCATGGAAAACGAGCTACTGCGGGAGAAAGCGAGGCGTGCCCACCC
TTTGGCCCAGAGGAGATCGAAGAAATGAGTGCTACGATCTCCCCTGCCACCGGTAAAGCCTATGGCGTTCAGCGCGTCTGCCTTGCCTGGGGAGTGCCCC
GCTCGTCGTTCTATAGCCGTGCAGGCAGAAAAACATTGCCTGCTGTACTGCTCAAGCGTGGTCCAAAAACACCGCTCTCTGACGACGAGGTACTTTCTCT
CATCCGGACCGACCTGGAGACATCCCCCTTTGTCGGCGAAGGGCACCGTAAGGTCTGGGGACGACTACGCTTCGTCAAAGGGATAAAAGTCGGCCGCAAA
CGAGTGTTGCGTCTCATGCGGGAGAACAATCTGCTCTCCCCTCACCGGGTTGTTCAGGGACAGCCCAAGGACCACGCCGGCAAGATCATCACGGCGGCCC
CCAACGTCATGTGGGGTACCGATGGCACCAAGATCTTCACCCTGGAAGAAGGGTGGTGCTGGTTGTTTACCGCCGTCGAGCATTGGAATGCCGAGTGCGT
CGGTTGGCATGTGACCAAGAATGGCGACCGGTTTGCCGCATTGCAGCCGCTCGCCATGGGAATCAAGGCCCGTTTCGGCTCGGTCGGTGCCGCTGCTGCT
CGAGGGTTGGCACTGAGGATGGATCACGGCAGCCAATACCTCTCAGAGCATTTCCAGAATCAGATCAAGTTCTGGGGAATTGCCCCAAGTTTCTCATTTG
TTGCCGAACCGCAGACCAACGGGGTGACGGAACGGTTTAATCGGACTATCAAAGAGCAGGTCATCTATGGCCGCCATTACCGCACAATCGAAGAAGTAAG
GATGGCAGTAGCTGACTTCATGGATCGTTACAACCGGCTGTGGCTGGTTGAAAAGCTCGGGTTCAGAAGCCCACGACAGGCTTTCGAGGAGTATCAACTC
AAGAAGGCTGCGTGACTCTTATCTTGTGTCCAGGGTACCGGGCGCGCTACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : Yes
- Secondary structure : stem-loop
Recoding motif :
GAGGCGTGCCCACCCTTTGGCCCAGAGGAGATCGAAGAAATGAGTGCTAC
--------------------------((((((((-((---------))-)
GATCTCC
)))))))
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
363 bp | 120 aa | 66 | 428 | + | No |
Description : First part of the transposase
ORF sequence :
MEKDVSVSGAAEGERSSTGAAPEVKRWSANRKKEVVLRLMRGEPIDALSRELGIEIYRLEEWHQKALQGIESALKAREGDPLAAELDAAFKRIGEITMEN
ELLREKARRAHPLAQRRSKK
ELLREKARRAHPLAQRRSKK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
966 bp | 296 aa | 350 | 1315 | + | No |
Description : Second part of the transposase
ORF sequence :
DHHGKRATAGESEACPPFGPEEIEMSATISPATGKAYGVQRVCLAWGVPRSSFYSRAGRKTLPAVLLKRGPKTPLSDDEVLSLIRTDLETSPFVGEGHRK
VWGRLRFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPL
AMGIKARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVE
KLGFRSPRQAFEEYQLKKAA
VWGRLRFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPL
AMGIKARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVE
KLGFRSPRQAFEEYQLKKAA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1250 bp | 415 aa | 66 | 1315 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKDVSVSGAAEGERSSTGAAPEVKRWSANRKKEVVLRLMRGEPIDALSRELGIEIYRLEEWHQKALQGIESALKAREGDPLAAELDAAFKRIGEITMEN
ELLREKARRAHPFGPEEIEMSATISPATGKAYGVQRVCLAWGVPRSSFYSRAGRKTLPAVLLKRGPKTPLSDDEVLSLIRTDLETSPFVGEGHRKVWGRL
RFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPLAMGIK
ARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVEKLGFR
SPRQAFEEYQLKKAA
ELLREKARRAHPFGPEEIEMSATISPATGKAYGVQRVCLAWGVPRSSFYSRAGRKTLPAVLLKRGPKTPLSDDEVLSLIRTDLETSPFVGEGHRKVWGRL
RFVKGIKVGRKRVLRLMRENNLLSPHRVVQGQPKDHAGKIITAAPNVMWGTDGTKIFTLEEGWCWLFTAVEHWNAECVGWHVTKNGDRFAALQPLAMGIK
ARFGSVGAAAARGLALRMDHGSQYLSEHFQNQIKFWGIAPSFSFVAEPQTNGVTERFNRTIKEQVIYGRHYRTIEEVRMAVADFMDRYNRLWLVEKLGFR
SPRQAFEEYQLKKAA
Blast result :
Comments
Two of the ten insertions in this strain lack a direct repeat.
ISGsu7 is 61% aa similar to ISMtsp5.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
ISGsu7 is 61% aa similar to ISMtsp5.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
References
1] Methe BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, Dodson RJ, Madupu R, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Gwinn M, Kolonay JF, Sullivan SA, Haft DH, Selengut J, Davidsen TM, Zafar N, White O, Tran B, Romero C, Forberger HA, Weidman J, Khouri H, Feldblyum TV, Utterback TR, Van Aken SE, Lovley DR, Fraser CM. (2003) Science 302 (5652), 1967-1969