ISfinder Mirror Site

ISGsu6

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AE017180	ND	Geobacter sulfurreducens	Geobacter sulfurreducens PCA

DNA section

IS Length : 1975 bp

Ends

IR Length : 24/29

IRL : TGTTAGCGGCAATGTAAAAATGACCCACTTCGGAAATCGAAAATTGACCC
IRR : TGTCAACGGCAATCGAAAATTGACCCACTTTACCGGTTGATCGGAAATTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GACTTGGAAA	GCCCAT	GGTCAATTGA	6

DNA sequence

TGTTAGCGGCAATGTAAAAATGACCCACTTCGGAAATCGAAAATTGACCCATTCTTCCTCTATCCTAGCCGACCTGGAGGGTCGGCATGATCGGAAAGGA
AGAGTGTATGGAAGTCAGAATTCTCAAGAAGCAGGGCAAGAGCATCCGGGAGATCTCGCGTATCACCGGACTTTGCCGCAACACGGTCAGGAAATTCCTC
AGGTCGACCGCTGATCCCAGGTACAAAGAACGAGCGAAGCGACCGGGGAAGCTTGACCCATTTAAAGCGTTTCTCGAAGAACGGGTGAAGGCGGCTCTGC
CGCATCGGATTCCCGCGCCGGTGCTTGCTCGGGAGATCAAAAGCTTTGGCTACGATGGCGGCGAACGCACTGTCAGAACGTTTTTGGCGACCCTTTATTC
CCGCATCACGCCGGAACCCGTTGTCCGGTTCGAAACGGAGCCAGGCAAGCAGATGCAAGCCGACTGGTGCGAACTGCGCAAGGGCAAGCACCCGCTGTAC
GCATTCGTTGGAACGCTTGGTTTCAGTAGAGATTCGTTCGTTGTCTTCACCACCAGCCAAGCGTTCGACGTCCTTCGGGAATGCCACGAGATGGCATTCT
CCTTCTTCGGCGGGGTCCCGCGCGAAGTGCTCTATGACAACATGAAGACCGTCGTGCTGGAGCGAAACGCCTTCGGCGAGGGCAACCACCGTTTCCACAC
CGGCCTGTGGGACACGGCAAAGCATTTCGGGTACATCCCCCGGTTGTGCAAGCCGTACCGGGCCAAGACCAAGGGAAAGGTTGAGCGGTTCAACCGCTAC
CTGCGGTACAGCTTCTACTACCCGTTGGTTTCGCGCCTGAAGCAGGCAGGACTAACTCTCGATGTCGCAACCGCGAACATCGAGGTCAGAAAGTGGCTCA
ACGAAGTAGCCAACTGCCGCATCCATGGGGAGACCGGCGAACGCCCCTGCGATCGGCTCGAGATCGAGCGGTCAGCGATGCTGCCGCTGCCGCCAAGGCT
TGCAGTCGAGCCGGACAAGCCGCAAGTCATTGCTGCCATGCCGTGGCCGGTTATTCCCCTTCAGCGCCCCGCCTCCTTCTACGATCAGATCCTTCAGGAG
GGGCGGCTATGAGCGACATCCAGCACCAGCGTATGGTCACGCTCTGCGATGATCTGAAGTTTCTGTCGGTGGCCGATGTCTACACCGACCTTGCAGATGC
CGCGGCAAAGCAGGAATCCTCCTACATCGACTACCTTGAACAGGTACTCAAGGCCGAGAACGACGTCCGACAGGGACGCTCGCGCCACACAATGGCAAAG
CTCGCCGGTTTTCCCGCGATCAAGACTCTCGAGGATTATGACTTTGAGTTCGCCACCGGCGCCCCGAAACAGCGAATTCTGGACCTGTCGGCGATGGCAT
TCCTCGAGCGCCGGGAGAATGTAATCCTGCTGGGTCCCAGCGGCACCGGAAAGACTCACCTCGCCATCGCGCTCGGCTACCGCGCTACTCAGTGCGGCGT
GAAGGTACGCTTCATCTCCGCCGCCGACCTGATGCTGCAACTTGAAAGCGCCCAGCGGCAGGGGCGGTACAAGGAAGTAATGCGGCGCAGTGTCCTGGGG
CCGAGACTGCTCATCATCGATGAGATCGGCTACCTCCCGTTCAGCGAAACGCAGGCGAATCTGTTCTTTCAGGTGATCGCCAAAAGGTACGAAACAGGTT
CCGTCATCCTCACTTCGAACCTGAGCTTCGGGGAATGGGAACAAGCCTTCGGCGGCAATACGGCACTCACATCAGCTATGCTCGACCGACTGCTGCACCA
CGCCCATGTCATTCAGATCAGAGGTGACAGCTACCGGCTGAAAGAGAAGCGACGCGCAGGTATTCTCGGCCAGCAACTGCCGACACCTCAGATCGATGAC
TAATCCAGGAAGGTGGGTCATTTTTCAATTTCCGATCAACCGGTAAAGTGGGTCAATTTTCGATTGCCGTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1026 bp	341 aa	87	1112	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIGKEECMEVRILKKQGKSIREISRITGLCRNTVRKFLRSTADPRYKERAKRPGKLDPFKAFLEERVKAALPHRIPAPVLAREIKSFGYDGGERTVRTFL
ATLYSRITPEPVVRFETEPGKQMQADWCELRKGKHPLYAFVGTLGFSRDSFVVFTTSQAFDVLRECHEMAFSFFGGVPREVLYDNMKTVVLERNAFGEGN
HRFHTGLWDTAKHFGYIPRLCKPYRAKTKGKVERFNRYLRYSFYYPLVSRLKQAGLTLDVATANIEVRKWLNEVANCRIHGETGERPCDRLEIERSAMLP
LPPRLAVEPDKPQVIAAMPWPVIPLQRPASFYDQILQEGRL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
795 bp	264 aa	1109	1903	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSDIQHQRMVTLCDDLKFLSVADVYTDLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFEFATGAPKQRILDLSAMAFLE
RRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVI
LTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGDSYRLKEKRRAGILGQQLPTPQIDD

Blast result :

Comments

Only one of the two insertions in this strain displays a direct repeat.

References

1] Methe BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, Dodson RJ, Madupu R, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Gwinn M, Kolonay JF, Sullivan SA, Haft DH, Selengut J, Davidsen TM, Zafar N, White O, Tran B, Romero C, Forberger HA, Weidman J, Khouri H, Feldblyum TV, Utterback TR, Van Aken SE, Lovley DR, Fraser CM. (2003) Science 302 (5652), 1967-1969