ISfinder Mirror Site

ISGsu5

Family IS110
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AE017180	ND	Geobacter sulfurreducens	Geobacter sulfurreducens PCA

DNA section

IS Length : 1400 bp

Ends

Left end : GGATACTATGTAAGGCCCCCCTTGGGCATACGGATTTTTGCCGTATGCTGGACTTTGCGAGAACCCATTCTCCAAAGGAGGCCTTACCCATGAGATTTTA
Right end : GTCAGAACAGAGAAACTGCTGATCCGATTCATTTAAAAAGCGATTCAAAATCGTCAGGGCTCTTTTTTGCCCTTGACGGACGGGGGCCTAATAAGGGTTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

GGATACTATGTAAGGCCCCCCTTGGGCATACGGATTTTTGCCGTATGCTGGACTTTGCGAGAACCCATTCTCCAAAGGAGGCCTTACCCATGAGATTTTA
CACAAAAACTCACAAACACTACTGCGGAATCGATCTTCATGCGAAGAAAATGTTCCTCTGTATCCTGGATGCACAGGGCGAGGTGCTCCTGCACCGCAAC
ATCTCAAGTTCGCCGGAGGCCTTTCTCAAAGCAGTCGCGCCTTTCCGTGATGGCTTGGTGGTCGGCGTGGAATGCATGTTTGCCTGGTACTGGCTTGCCG
ATCTCTGCCGTAAGGAAGGAATCGAATTTGTCTTGGGTCATGCCCTTTACATGAAAGCCGTCCACGGCGGGAAAGCGAAAAACGACAAGATCGATTCCCA
CAAGATCGCGGTGCTGTTACGCGGCGGCTCGTTCCCTGTTGCTTACGTCTACCCTCCGGAGATGCGCGCTACCCGCGATCTGCTGCGCCGTCGGACCCAT
ATCCACGGTGTCGGGCAAATCCTGGCCCTGGTGATGCTCTACGAGATCCACGACATCAATCGTTTTGAGTCGGTTCAGGATTTCGCCTCTTACTGCCGCT
TAGTCAAATGCAGTCACGAATCAGCCGGGAAGAAGAAAGGCAGCGGCGGCGCCAAGATCGGCAATGCGCATCTGAAGTGGGCCTTTTCCGAAGCAGCCAT
GCTGTTCATCCGTTACAACCCGCCGGCCAAGAAGCTGGTGGAGAATCTGGCCAAGGTACATGGCAAGGGAAAGGCTATCGGAATCATAGCGCACAAGCTG
GCGCGGGCGATCTATTACATGCTGAAAAATAACGTGCCGTTCAACTTGGAGAAGTTCATGGCAACAGCATAGAGCTGGAGGGGATGGAAGCAGCTACAAC
GTTCACCTGGAGCCACATGGGGCGAGCATGATCACGACCGTGAAAGATGAGTAACAGTGCGTCTTTGAACGCGAACCGGTCGATTGGCCGATGCCCGGAT
ACCTGTCGCTTGGATTAGAGCTCCGTCCCGCTCCTTACATACATGAGTCGATTTCTTGCTGTTTCGGTGTGTTCTGCCCCTCGCCTGAGCCCGGCACTAA
CTGCGATTCTCCGTCAGGATTAATCCCGACGCTTTGAATGGGACGGCATGAGGGTACGGTACAGTTTCCAGGTCGCGAAGGTGATGCCTTCGCAGCCGCT
GCCAATTGTAGTATCCGCAGCGGTGTGAACCTCGATAGGTGTTTGGTGCAGGACCTTTCCTTCTGGCAAAGGTTCCTGACTCTGATGATTCCGAAATGAA
GTCAGAACAGAGAAACTGCTGATCCGATTCATTTAAAAAGCGATTCAAAATCGTCAGGGCTCTTTTTTGCCCTTGACGGACGGGGGCCTAATAAGGGTTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
783 bp	260 aa	90	872	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MRFYTKTHKHYCGIDLHAKKMFLCILDAQGEVLLHRNISSSPEAFLKAVAPFRDGLVVGVECMFAWYWLADLCRKEGIEFVLGHALYMKAVHGGKAKNDK
IDSHKIAVLLRGGSFPVAYVYPPEMRATRDLLRRRTHIHGVGQILALVMLYEIHDINRFESVQDFASYCRLVKCSHESAGKKKGSGGAKIGNAHLKWAFS
EAAMLFIRYNPPAKKLVENLAKVHGKGKAIGIIAHKLARAIYYMLKNNVPFNLEKFMATA

Blast result :

Comments

Closely related to ISGsu4 in this strain, which was used to identify the right end-sequence. One copy of ISGsu5 is intact and one is truncated in this strain.

References

1] Methe BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, Dodson RJ, Madupu R, Brinkac LM, Daugherty SC, DeBoy RT, Durkin AS, Gwinn M, Kolonay JF, Sullivan SA, Haft DH, Selengut J, Davidsen TM, Zafar N, White O, Tran B, Romero C, Forberger HA, Weidman J, Khouri H, Feldblyum TV, Utterback TR, Van Aken SE, Lovley DR, Fraser CM. (2003) Science 302 (5652), 1967-1969