ISBlo4
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004307 | ND | Bifidobacterium longum | Bifidobacterium longum NCC2705 |
DNA section
IS Length : 1399 bp
Ends
IR Length : 27/31
IRL : GGCGGGTTCTAGTGATCGTTGCAACACCTGACCTACCGCAAGGAGGGTCA
IRR : GGCAGATTCTAGTGGTGGTTGCAACACCTGAGGTTTGAGCGGCCTTCCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATCCACAT | AAA | CAGATAAGGC | 3 |
DNA sequence
GGCGGGTTCTAGTGATCGTTGCAACACCTGACCTACCGCAAGGAGGGTCAGGTGACCAGAAGCCACCGAAACGAAGACCGTGGCGGGGAACACCGTTGGA
CGTTCAACGGCGTCGAATATCCGACGAGGAAGCTGATGTGCGAGGCGAGACGCGCCGAGTACGTGCGCCTGCTGGACGAGGAAGGCATGAACTTCACCCA
GGCCGCGCACGCGGTCGGCGTCTCGAAACGCACCGGCAAGGCGTGGCGCAACGGCAGGACGCGCGCCACGGGAAGGAACGAGAAACCCCTGGTGGACTGG
TATCGTTCCACCATGGACAAACCCAAGACCCTCCATCCGCGCTACCTGAGCCAGGAGGAGCGCATCCAGATCGCGGACCGTCTGCGTCTGGGCGATTCGA
TCCGCGCCATCGCCCGCCTGCTGGGCCGCGACCCCGGCACGGTCAGCCGCGAGGTCGAGCGCAACAGGAATCCCGAGTCCGGCGGTTACGAGCCTTACCG
CGCCCAGCAGAAGGCCGCGGACCGGCTCAAACGCCCCAAACCGCGCAAGGCGGCCGAGGGCACGCGACTGTGGGACGAGATCGCCGCCGGGTTGCGCAGG
CATTGGAGCCCGGAGCAGATAGCCAACCGGCTGAGGCTGGACTTCCCGGATAATGGGGATATGCACGCGAGCGTCGAGACGATCTACCAGGCCATCTACC
TGCAGGCCAGGGGCGAACTCAAGCAGGAGCTGAAACGCGCCATGAGGCAGGGGCGAACCGCCCGCAGACCCCAAGGCGGCCAAGGCCGCAAACCCCGTTT
CCGCGAACCCATGGCCATGATCTCGGAGCGACCCCCGGAGATCGAGGACCGGGCGGTCCCGGGCCACTGGGAGGGCGATCTCATCACCGGCAGCCGCAAC
AAAAGCGCGATCGGCACGCTCGTCGAGCGCACCACCAGGTTCACGATCCTGCTGCACCTGCCCGACGGGCACGACGCCGAACACGTCCAGCAGGCCATCA
TCGACAAGATGCAGCACCTGCCCAAACTCCTGCGCAACAGCCTGACCTGGGACCAGGGAGCGGAACTCGCCCTGCACAAACGGATCGGCGCCTCGCTGGA
CATGGCCGTCTACTTCTGCGACCCGCACTCCCCGTGGCAGCGCGGCACCAACGAGAACACCAACGGGCTCCTGCGCCAGTACTTCCCCAAAGGCACCGAC
CTATCCGTCTACCCGGAGGACTACCTCGACGCGGTCGCCGAGGAACTCAACGACCGGCCACGCAAAACCCTCGGGTTCATGAAACCAAGCGAGAAGATCA
TCGAACTGCTCGACGCCGCGTGATAACCTCAACAACCGACAACGTGACCATGGAAGGCCGCTCAAACCTCAGGTGTTGCAACCACCACTAGAATCTGCC
CGTTCAACGGCGTCGAATATCCGACGAGGAAGCTGATGTGCGAGGCGAGACGCGCCGAGTACGTGCGCCTGCTGGACGAGGAAGGCATGAACTTCACCCA
GGCCGCGCACGCGGTCGGCGTCTCGAAACGCACCGGCAAGGCGTGGCGCAACGGCAGGACGCGCGCCACGGGAAGGAACGAGAAACCCCTGGTGGACTGG
TATCGTTCCACCATGGACAAACCCAAGACCCTCCATCCGCGCTACCTGAGCCAGGAGGAGCGCATCCAGATCGCGGACCGTCTGCGTCTGGGCGATTCGA
TCCGCGCCATCGCCCGCCTGCTGGGCCGCGACCCCGGCACGGTCAGCCGCGAGGTCGAGCGCAACAGGAATCCCGAGTCCGGCGGTTACGAGCCTTACCG
CGCCCAGCAGAAGGCCGCGGACCGGCTCAAACGCCCCAAACCGCGCAAGGCGGCCGAGGGCACGCGACTGTGGGACGAGATCGCCGCCGGGTTGCGCAGG
CATTGGAGCCCGGAGCAGATAGCCAACCGGCTGAGGCTGGACTTCCCGGATAATGGGGATATGCACGCGAGCGTCGAGACGATCTACCAGGCCATCTACC
TGCAGGCCAGGGGCGAACTCAAGCAGGAGCTGAAACGCGCCATGAGGCAGGGGCGAACCGCCCGCAGACCCCAAGGCGGCCAAGGCCGCAAACCCCGTTT
CCGCGAACCCATGGCCATGATCTCGGAGCGACCCCCGGAGATCGAGGACCGGGCGGTCCCGGGCCACTGGGAGGGCGATCTCATCACCGGCAGCCGCAAC
AAAAGCGCGATCGGCACGCTCGTCGAGCGCACCACCAGGTTCACGATCCTGCTGCACCTGCCCGACGGGCACGACGCCGAACACGTCCAGCAGGCCATCA
TCGACAAGATGCAGCACCTGCCCAAACTCCTGCGCAACAGCCTGACCTGGGACCAGGGAGCGGAACTCGCCCTGCACAAACGGATCGGCGCCTCGCTGGA
CATGGCCGTCTACTTCTGCGACCCGCACTCCCCGTGGCAGCGCGGCACCAACGAGAACACCAACGGGCTCCTGCGCCAGTACTTCCCCAAAGGCACCGAC
CTATCCGTCTACCCGGAGGACTACCTCGACGCGGTCGCCGAGGAACTCAACGACCGGCCACGCAAAACCCTCGGGTTCATGAAACCAAGCGAGAAGATCA
TCGAACTGCTCGACGCCGCGTGATAACCTCAACAACCGACAACGTGACCATGGAAGGCCGCTCAAACCTCAGGTGTTGCAACCACCACTAGAATCTGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1272 bp | 423 aa | 52 | 1323 | + | No |
Chemistry : DDE
ORF sequence :
MTRSHRNEDRGGEHRWTFNGVEYPTRKLMCEARRAEYVRLLDEEGMNFTQAAHAVGVSKRTGKAWRNGRTRATGRNEKPLVDWYRSTMDKPKTLHPRYLS
QEERIQIADRLRLGDSIRAIARLLGRDPGTVSREVERNRNPESGGYEPYRAQQKAADRLKRPKPRKAAEGTRLWDEIAAGLRRHWSPEQIANRLRLDFPD
NGDMHASVETIYQAIYLQARGELKQELKRAMRQGRTARRPQGGQGRKPRFREPMAMISERPPEIEDRAVPGHWEGDLITGSRNKSAIGTLVERTTRFTIL
LHLPDGHDAEHVQQAIIDKMQHLPKLLRNSLTWDQGAELALHKRIGASLDMAVYFCDPHSPWQRGTNENTNGLLRQYFPKGTDLSVYPEDYLDAVAEELN
DRPRKTLGFMKPSEKIIELLDAA
QEERIQIADRLRLGDSIRAIARLLGRDPGTVSREVERNRNPESGGYEPYRAQQKAADRLKRPKPRKAAEGTRLWDEIAAGLRRHWSPEQIANRLRLDFPD
NGDMHASVETIYQAIYLQARGELKQELKRAMRQGRTARRPQGGQGRKPRFREPMAMISERPPEIEDRAVPGHWEGDLITGSRNKSAIGTLVERTTRFTIL
LHLPDGHDAEHVQQAIIDKMQHLPKLLRNSLTWDQGAELALHKRIGASLDMAVYFCDPHSPWQRGTNENTNGLLRQYFPKGTDLSVYPEDYLDAVAEELN
DRPRKTLGFMKPSEKIIELLDAA
Blast result :
Comments
The genome of NCC2705 shows two copies of this element.
Coordinates:
ISBlo4a complement(99388..100786)
ISBlo4b complement(865210..866608)
DR :
ISBlo4b shows ambiguity in its DRs: a supernumerary G (sequencing mistake?) in the left DR prevents the identification of a 4 bp DR.
L-side ggaggggcgc(caag)[g]
R-side (caag)ccatgctaaa
Identity between IS copies:
The two copies are 100% identical.
Coordinates:
ISBlo4a complement(99388..100786)
ISBlo4b complement(865210..866608)
DR :
ISBlo4b shows ambiguity in its DRs: a supernumerary G (sequencing mistake?) in the left DR prevents the identification of a 4 bp DR.
L-side ggaggggcgc(caag)[g]
R-side (caag)ccatgctaaa
Identity between IS copies:
The two copies are 100% identical.
References
1] Schell,M.A., Karmirantzou,M., Snel,B., Vilanova,D., Berger,B., Pessi,G., Zwahlen,M.-C., Desiere,F., Bork,P., Delley,M., Pridmore,D. and Arigoni,F. (2002)Proc. Natl. Acad. Sci. U.S.A. 99 (22), 14422-14427