ISBlo2

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_004307 ND Bifidobacterium longum
Bifidobacterium longum NCC2705
Bifidobacterium longum subsp. infantis 157F
DNA section
IS Length : 2455 bp

Ends


IR Length : 51/76

IRL : TGTTTGTGTTCAATTAGTTGTTGCGGGTTTAGTCACGATGATTTTTCGGG
IRR : TGTATTTCGTCAAGTCGGTGTTTCGTGTTTAGTCATGTATTTTTTTCGGG

Insertion site


Left flankDirect repeatRight flankDR Length
CGGCAAGACCCATCTCGCGGTCGCGTTG8
GTGCATCATCCT/ACGAGCACAGGCGGT8

DNA sequence

TGTTTGTGTTCAATTAGTTGTTGCGGGTTTAGTCACGATGATTTTTCGGGTTTAGGCAGAGCATGCCGACCACGGAATGGTCTTAACTGGATGGTGCTTG
TATCTCCGCCAGAAAGGACCGGAAAGGATTATGATCAGCATGCCCCAAGTACAGTCTATCCGTCGTTTGCGGCGGAACGGGGAATCCGTCGCGTCGATCG
CGCGGAAAACACATGTCAGCGAGCCGACGGTGCGTAAGTACCTCGCGAAGGAGGATCTGTCGGCCGTGCCGTCGGTGAGAAAGCCGCGCGCGTCGGTGAT
CGACCCGTATCTGCCCGTGATCGAGCAGTGGCTCGCGGAGGATCGCGCGAACTGGCGCAAGCAGCGGCATACCGCGACCAGGATCTGGGAGCGGTTGAGG
GACGAGCATGGCGCGGAGGTCTCCCTGTCCACGGTGACGCGGGCGGTGGCGCGGCTCAGGCGTGAGTTCGCGGCGGAGCGTGATGAGGCGTTCATGGATC
TGGTATGGCATCCAGGCGAGGCGCAGGCCGATTTCGGCGAGGTCGACGTGCTGTTGCGCGGCGTGGTGCAGCGGATGCATCATTTCGTGCTTGATTTCCC
GTATTCGAACGTCGGTCTGGTGCAGTTGATGCCGGGCGAGAACGCTGAGTGCACGTGCCTGGCGTTGCGCAACCTGTTCGAGTGGCTGGGCGGTGTGCCG
GAGCGGATCGTGTTCGATAACGCCGCCGGCGTGGGCCATAAGGGGTACGGGGAGCGCGAGCCGCGCCTGACGCACCTGTTCCAGGCCTTCCAGGCGCATT
ACGGGTTCGATTGTTCGTTCTGCAACCCGTATTCCGGTCATGAGAAGGGCGCGGTGGAGTCCAAGGTCGGCATGGTGCGGCGCAAGCTGTTCGTGCCCCG
GCCGAGCGTATGGAGCCTGGAGAACTTCAGCGCCGGGCTGCCGGACCGGTGCCTGGAGCTGGCCACGAAACCGCATTACCGCAAGGACACGGAGGAACGT
GGCCTGTTCTCCGAGGACCGTGCGGCGCTGCTGCCGTTGCCCGGGAAGCGTTTCGACGTGGTCACGTGGCGGCATATGAAGGCCGACCGGTACGGCGTGG
CCGCGCCCGGGGACGGCATCGCTGTTCCGCGGACCGGGCGAACGCGGGCAGAGGGGTGATCGTGGGCCTCCGCGCGCTCGAGGTCGAGGTCCTCGACTCG
TCGGGCAAGCATCTGGCGACCCATCCTCGGGCGTACGGCCAGGCCAGCACGAACAGCGAGGACCCGTCCATGCAGTTGGCGCTGCTGTGCAACAAGCCCG
CGTCCTGGCCCAACAGCCGGGTACGTGACGCGTTGCCGGACCCGTTGCGGGAATGGCTGGATCGGCAGGACAGACAGACACGCAACGAGGCGCTGCAGAC
CCTCAAACGCGTTGACAGGGAATCCGGGTGGGCGAACGCGGTCGAGGCGATGCTTTCCATCCTCGAATCGACCGGCGGGGCGGACCGCGCCGGTGTCACA
CTGCTCGCGGCCAGGCTCGCCGAGGGCGTGGCGGGTATCGAATACGACGATGACAGGCCGGATCCGGGTGAATACGACATCGCGTTCACCGCGGACGTCG
GCGTGCAGGAAGGCGGGCGATGAACACGAAGACGGACGAAGGGCTCTACGAGAAGGCCCGCAAGCTGTTCATCTCCAAGGCGAGCATCGACGAATTCGCC
GGCTGGGCCACGCCCCGCCAGGTCGACGCCGTCCACCGCCTGCTCGACACGGAACTGGCGAACAGGGAACGCGCGAAGCACGACAGGCTCCTGCGCCGCG
CCCGGTTCCCCGTCGTCAAGGGTCTCGACGGCTACGACTTCACGAACGTCAGGCTCCCCGACGGCTACATGCTCGATGAGCTCCTAGGGCTCGGTTTCAT
CCCGCGCGCGCAGGACCTGGTGTTCTACGGCAAGACCGGGCGCGGAAAGACACATCTCGCGATCGGGCTGGGCATGAAGGCGATCGACATGGGGCTGGGC
GTGCGGTTCCATCAGACCGCCGAGCTCGTCCTCCAGCTGGGCAAGGCCAAACGCGACGGCACGCTCGAGACGATGCTCCGGGACATCGGCCGTGCCGACC
TGATCATACTGGACGAGTTCGGCTACGTACCCTTCGACATCGACGGGGCGCGCCTGCTCTACCGGATCATCGCGGGCAGCTACGAAAGACGGAGCATCAT
ATTCACCACGAACATCGAGTTCAGTAAATGGGGCACGGTCTTCGCGGACGACAAACTCGCCGCAGCGATCATCGACCGCATCGTGCACCACGGGCGGCTC
CTCGAGTTCACCGGCCAGAGCCGCCGCGTCAGTGAGGCCCTCATGTTCGGCAGGAACACCTCCGCGCAACCGGAAAAATAAGGAAAATGCATGCAACTGC
CTAAACCCGAAAAAAATACATGACTAAACACGAAACACCGACTTGACGAAATACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1484 bp494 aa1401623+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPQVQSIRRLRRNGESVASIARKTHVSEPTVRKYLAKEDLSAVPSVRKPRASVIDPYLPVIEQWLAEDRANWRKQRHTATRIWERLRDEHGAEVSLSTVT
RAVARLRREFAAERDEAFMDLVWHPGEAQADFGEVDVLLRGVVQRMHHFVLDFPYSNVGLVQLMPGENAECTCLALRNLFEWLGGVPERIVFDNAAGVGH
KGYGEREPRLTHLFQAFQAHYGFDCSFCNPYSGHEKGAVESKVGMVRRKLFVPRPSVWSLENFSAGLPDRCLELATKPHYRKDTEERGLFSEDRAALLPL
PGKRFDVVTWRHMKADRYGVAAPGDGIAVPRTGRTRAEGVIVGLRALEVEVLDSSGKHLATHPRAYGQASTNSEDPSMQLALLCNKPASWPNSRVRDALP
DPLREWLDRQDRQTRNEALQTLKRVDRESGWANAVEAMLSILESTGGADRAGVTLLAARLAEGVAGIEYDDDRPDPGEYDIAFTADVGVQEGGR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
762 bp253 aa16202381+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNTKTDEGLYEKARKLFISKASIDEFAGWATPRQVDAVHRLLDTELANRERAKHDRLLRRARFPVVKGLDGYDFTNVRLPDGYMLDELLGLGFIPRAQDL
VFYGKTGRGKTHLAIGLGMKAIDMGLGVRFHQTAELVLQLGKAKRDGTLETMLRDIGRADLIILDEFGYVPFDIDGARLLYRIIAGSYERRSIIFTTNIE
FSKWGTVFADDKLAAAIIDRIVHHGRLLEFTGQSRRVSEALMFGRNTSAQPEK

 

Blast result :
Comments
References
1] Schell,M.A., Karmirantzou,M., Snel,B., Vilanova,D., Berger,B., Pessi,G., Zwahlen,M.-C., Desiere,F., Bork,P., Delley,M., Pridmore,D. and Arigoni,F. (2002)Proc. Natl. Acad. Sci. U.S.A. 99 (22), 14422-14427