ISfinder Mirror Site

ISPsy14

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AE016853	ND	Pseudomonas syringae	Pseudomonas syringae DC3000 Pseudomonas putida S12 plasmid pTTS12

DNA section

IS Length : 2597 bp

Ends

IR Length : 40/49

IRL : TGGGGATTCCATGACCATCCGGACACCCATTCCACCGACATCCGGACACT
IRR : TGCGGATTCCACGCCATCTGGACACTCAGCCCACCAACATCCGGACACCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATGCACTAT		CACCATGAAC	0
TCGAAAAGTA	GCCCTGCC	ACTTCGCGAG	8
TGGATACGCT	GATAGAT	CTCTTCGCGA	7

DNA sequence

TGGGGATTCCATGACCATCCGGACACCCATTCCACCGACATCCGGACACTGATTCCACGCTGATCCGGACACTCATTCCACAAGCATCCGGACACCGATT
CCACGGCCATCCGGACACTTTCAGGCAGGCAGCAACGCAGGACTATTCACTACTATCGACCTCTTTTTTCGAAGCTAAGAGGTCGTCGTGGAGCGCTTAT
CCATGCGTAAAATCCGAGAGGTACTACGCCTAAAGTTCGACGCCGGCCTGTCCGTGCGCAAGATCGCCGCCAGCTTATCCATCGGCCATTCGAGTGCAGG
GGATTACCTTTGCCGGTTTGCCGCCAGTGGGCTTCAGTGGCCCATCGATATTTCTGACGCTGAGTTGAATCGACGGCTGTTTCCACCAACAGTGCCGGTG
CCGACCGACCAGCGCCCAATGCCCGACTGGGCATGGGTACACACCGAGTTGCGCCGCCCCGGCGTAACCCTGGCCCTGCTCTGGCAGGAATATCGACTCG
CTCACCCGCAAGGTTTTCAATACAGCTGGTTCTGCGAGCACTACCGCCTCTGGGCCGCCAAGGTCGACGTGGTCATGCGTCAGGAACACCGTGCTGGCGA
AAAGCTGTTCGTTGATTACGCCGGGCAAACAGCGCCGGTCATCGACCGGCAAACCGGCGAGATCCGCCAAGCGCAGATCTTCGTCGCCGTTCTCGGCGCG
TCCAGCTACACCTTTGCCGAGGCCTCCTGGTCGCAGAAACTGCCCGACTGGCTGGGCTCGCACGTCCGATGCTTTGCTTTTTTCGGCGGCACAACGCAGA
TCCTGGTGCCCGATAATCTGCGCAGTGGCGTGACTAAGGCGCACCGTTACGAGCCGGATATCAACCCCAGTTACCGCGATCTGGCCGAGCATTACGGCGT
CGCCGTCTTGCCTGCTCGCTCGCGTAAGCCCAAGGACAAAGCCAAAGTCGAAGTCGGCGTGCAAGTAGTCGAACGCTGGATCCTGGCGGTGCTGCGCAAC
CGCCAGTTCTTCTCGCTTGGCGAATTCAATACAGCCATCGCGCTACTGCTGGATCGCCTCAACCACAAGCCCTTCAAAAAGCTGCCCGGCTCACGCCGGT
CAGCCTTCGAGGCCCTCGACCAACCGGCGCTGCAAGGGCTGCCGGAACACCCGTACGTCTATGCCGAATGGAAAAAGGTACGCGTCCATATCGACTATCA
CGTCGAGGTCGATGGCCACTTCTACTCGGTGCCGTACCAGCTGGTGAAGCACCAACTCGAAGTACGACTCACCGCCCAGACCGTCGAGTGCTTCCACGCC
AACCAGCGCGTGGCCAGCCACTTGCGCTCGCTGCACAAAGGCCGCCACACCACTCAAACCGAGCACATGCCCAAGAGCCACCGCGAACATGCTGAGTGGA
CGCCGCAACGGCTGATCCGCTGGGCAGAGCAGACCGGGCCCAACACCACTGGCGTCATCGCCTACATCCTCGAACGCCGAATCCACCCACAGCAAGGCTT
CCGCGCCTGCCTGGGCATCCTGCGCCTGAGCAAACAGCACGGCGAAGCGCGGCTGGAAGCCGCGTGCCAGCGTGCGCTGGCACTGGGCGCATGCAGTTAC
AAAAGCCTGGAATCAATCCTGCGCCAAGGGCTGGAGCGCCTGCCGCTGGCCCAGCAAAACCTGCCGCTACTGCCCGACGAGCACATCAACCTGCGTGGCC
CGGACTACTACCACTGACCTGAGAAAGGAGCTTCGAAATGCTACCGAATCCAACGCTCGACAAGCTACAAACCCTGCGCCTGCACGGCATGATCAAGTCG
CTGGGCGAGCAACACGCAACGCCCGATATCAATGACTTGAGCTTCGATGAACGCCTCGGCCTGATGGTCGACCGCGAGGTGACCGAGCGCGAAGACGCCC
GCCTGACCACTCGCCTCAAAGCGGCGCGGTTGCGCCACAACGCCTGCCTGGAAGACATCGACTACCGCAGCCCGCGTGGCCTGGACAAGGCCATGATCCT
GCAACTGGGCGGTGGCCAGTGGCTGCGTGATGGCCTGAACTTGATCATCGGCGGGCCAACTGGCGTAGGCAAAACCTGGCTCGCCTGCGCGCTGGCCCAC
AAGGCCTGCCGCGACGGCTACAGCGTGCGTTACCTGCGCCTGCCGCGCTTGATGGAGGAGCTGGGCCTGGCCCATGGCGACGGCCGCTTCGCCAAACTGA
TGGCCGGCTACGCCAAGACCGACCTGCTGATCCTGGACGACTGGGGCCTGGCACCGTTTACTGCCGCGCGACGACGCGACATGCTTGAGCTGCTGGACGA
CCGCTACGGCAACCGCTCGACACTGGTCACCAGCCAGATGCCGGTGGACAAATGGCATGCGCTAATCGGCGATCCGACCTTGGGCGACGCGATCCTCGAC
CGGCTGGTGCACAACGCTTATCGGATCGAACTGAAGGGCGAGTCGATGCGCAGACGCGCAACGAAATTGACGACGGCAGGGACTTCAGACTAACAATGCG
AACCTGCGTCGCTGCGCTCCGACTGCCTGTCCGGATGATCGTGGAACGGGTGTCCGGATGTTGGTGGGCTGAGTGTCCAGATGGCGTGGAATCCGCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1515 bp	504 aa	203	1717	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFDAGLSVRKIAASLSIGHSSAGDYLCRFAASGLQWPIDISDAELNRRLFPPTVPVPTDQRPMPDWAWVHTELRRPGVTLALLWQEYRLA
HPQGFQYSWFCEHYRLWAAKVDVVMRQEHRAGEKLFVDYAGQTAPVIDRQTGEIRQAQIFVAVLGASSYTFAEASWSQKLPDWLGSHVRCFAFFGGTTQI
LVPDNLRSGVTKAHRYEPDINPSYRDLAEHYGVAVLPARSRKPKDKAKVEVGVQVVERWILAVLRNRQFFSLGEFNTAIALLLDRLNHKPFKKLPGSRRS
AFEALDQPALQGLPEHPYVYAEWKKVRVHIDYHVEVDGHFYSVPYQLVKHQLEVRLTAQTVECFHANQRVASHLRSLHKGRHTTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTTGVIAYILERRIHPQQGFRACLGILRLSKQHGEARLEAACQRALALGACSYKSLESILRQGLERLPLAQQNLPLLPDEHINLRGP
DYYH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
756 bp	251 aa	1738	2493	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPNPTLDKLQTLRLHGMIKSLGEQHATPDINDLSFDERLGLMVDREVTEREDARLTTRLKAARLRHNACLEDIDYRSPRGLDKAMILQLGGGQWLRDGL
NLIIGGPTGVGKTWLACALAHKACRDGYSVRYLRLPRLMEELGLAHGDGRFAKLMAGYAKTDLLILDDWGLAPFTAARRRDMLELLDDRYGNRSTLVTSQ
MPVDKWHALIGDPTLGDAILDRLVHNAYRIELKGESMRRRATKLTTAGTSD

Blast result :

Comments

There is 1 copy of ISPsy14 in the chromosome of this strain.
ISPsy14 is 94% (ORFA : the transposase) and 97% (ORFB : the helper of transposition) aa similar to ISPre4.

References

1] Buell,C.R., Joardar,V., Lindeberg,M., Selengut,J., Paulsen,I.T., Gwinn,M.L., Dodson,R.J., Deboy,R.T., Durkin,A.S., Kolonay,J.F., Madupu,R., Daugherty,S., Brinkac,L., Beanan,M.J., Haft,D.H., Nelson,W.C., Davidsen,T., Zafar,N., Zhou,L., Liu,J., Yuan,Q., Khouri,H., Fedorova,N., Tran,B., Russell,D., Berry,K., Utterback,T., Van Aken,S.E., Feldblyum,T.V., D'Ascenzo,M., Deng,W.L., Ramos,A.R., Alfano,J.R., Cartinhour,S., Chatterjee,A.K., Delaney,T.P., Lazarowitz,S.G., Martin,G.B., Schneider,D.J., Tang,X., Bender,C.L., White,O., Fraser,C.M. and Collmer,A. (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (18), 10181-10186 .