ISPsy8

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE016853 ND Pseudomonas syringae
Pseudomonas syringae DC3000
DNA section
IS Length : 1432 bp

Ends


IR Length : 23/25

IRL : TGGACTGCCCCCAAGAAGTTGGACACCAATCCGACCTAATGGGGGTGTTC
IRR : TGGACTGCCCCCCGGAAGTTGGACAGTTTTAGCTTGCCGCCTGAGTCCTG

Insertion site


Left flankDirect repeatRight flankDR Length
CCTCACGCTCCAGCGTAGGAATG3
TCCTCACGCTCTGCGTGGGAATG3
TGTGCCCCGTAAATGTGGGAGTG3
AAAGCTTGGCTTTTGTGGGAGTG3
CATTCCCACGCGGGGGGCGTAGG3

DNA sequence

TGGACTGCCCCCAAGAAGTTGGACACCAATCCGACCTAATGGGGGTGTTCAAATGGCCAAGTATTCAGAGCAATTCAAGCTCACCGTCGTCAAAGCCTAT
CTTGCAGGCAACATAGGCTTTCGCAAGGTGTCCAGCCAGTTCTCGATCGATTCCAGCCTGCTTCGACGCTGGGTCGCCAATTACAAGAGCCATGGGCACA
CAGGCCATCGTAAGCCTGGTCTTCGGTACAGCGAAGCCTTCAAACGCTCAGTGCTGGAGCACAAGCGCGAGCACGGGCTGTCCCTTCGCCAGACCGCAGC
GCATTTTGGTATTGGTGATCCTGGCCAAATAGTCATTTGGGAACAGCAGCATTACAGTAACGATCTAGCCCCCTACGTTCCCAAGAGAAGAAAGTCTGTT
GCCATGCCCAAGAAGCCTTATCCCACCGCACCAGTAATCGACGACGATACGCAGAAGACGCGTGACCAGTTAATGGCCGAGCTCGAATACCTGCGCATGG
AGAACGCTTACCTAAAAAAGTTGGAAGAGTTGAAGGAGCAGCAACGACGGCAAAAGAAAAAGCCCTGATCGTCAAACAACTGAGGAGCCGCTTCTCGCTC
TGCGCCCTTCTGACGCTGGCGGGCTTGGCGCGCAGTACGTTCTACTATCAGGTTCAGGTGCAATCCAGACCCGATCCAGATGCTGCTCTAAAGCAGGAGG
TAGAGCGGATTTATCACGAGGAGAGAGGTCTTTATGGGGCTCGGCGCATCACAGCGGTTATCCGCAACTCAGGCACGCTGGTCAACAAGAAGGTTGTGGA
GAGATTGATGGCTGAACTGGGTCTGCGCTCCGTTGTTCGACCAAAAAAATACCGTTCCTATAAGGGCACCGTCGGAAAAATTGCACCGAATTTGCTGGAG
CGCAATTTCACGGCGCAACGCCCGAACCAGAAATGGGTGACCGATGTGACCGAGTTTAAAGTGGCCAATCGAAAGCTGTATCTCTCGCCTGTGATGGACC
TGTACAACGGTGAGATCGTGGCGTACGAATTGTCTACCAGGCCGTGCTTTGAGCTGGTCACCAGTATGCTGGACAAGGCGTTGCAGCAGTTGCAAGACGA
GCCGAAGCTTGTGATGCACTCGGATCAAGGCTGGCAGTATCAACACGCGCAGTACCGTCAGAAACTGGCGGCAAAGGGTGTGAAGCAAAGCATGTCTCGT
AAGGGAAATTGCCTGGACAACGCTGCGATGGAAAGCTTTTTTGGTACGCTTAAGTCCGAGTTTTTCTACTTGAAACGATTTGAAAGCATAGAAGAGCTGA
CGGCAGGTCTGGAGGAGTACATCCGCTACTACAACCATGACCGCATTAAGCTGAAGCTAGGCGGCCTGAGCCCCGTAAAATACAGGACTCAGGCGGCAAG
CTAAAACTGTCCAACTTCCGGGGGGCAGTCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
516 bp171 aa53568+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAKYSEQFKLTVVKAYLAGNIGFRKVSSQFSIDSSLLRRWVANYKSHGHTGHRKPGLRYSEAFKRSVLEHKREHGLSLRQTAAHFGIGDPGQIVIWEQQH
YSNDLAPYVPKRRKSVAMPKKPYPTAPVIDDDTQKTRDQLMAELEYLRMENAYLKKLEELKEQQRRQKKKP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
939 bp312 aa4661404+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PVNGRARIPAHGERLPKKVGRVEGAATTAKEKALIVKQLRSRFSLCALLTLAGLARSTFYYQVQVQSRPDPDAALKQEVERIYHEERGLYGARRITAVIR
NSGTLVNKKVVERLMAELGLRSVVRPKKYRSYKGTVGKIAPNLLERNFTAQRPNQKWVTDVTEFKVANRKLYLSPVMDLYNGEIVAYELSTRPCFELVTS
MLDKALQQLQDEPKLVMHSDQGWQYQHAQYRQKLAAKGVKQSMSRKGNCLDNAAMESFFGTLKSEFFYLKRFESIEELTAGLEEYIRYYNHDRIKLKLGG
LSPVKYRTQAAS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1352 bp450 aa531404+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKYSEQFKLTVVKAYLAGNIGFRKVSSQFSIDSSLLRRWVANYKSHGHTGHRKPGLRYSEAFKRSVLEHKREHGLSLRQTAAHFGIGDPGQIVIWEQQH
YSNDLAPYVPKRRKSVAMPKKPYPTAPVIDDDTQKTRDQLMAELEYLRMENAYLKKVGRVEGAATTAKEKALIVKQLRSRFSLCALLTLAGLARSTFYYQ
VQVQSRPDPDAALKQEVERIYHEERGLYGARRITAVIRNSGTLVNKKVVERLMAELGLRSVVRPKKYRSYKGTVGKIAPNLLERNFTAQRPNQKWVTDVT
EFKVANRKLYLSPVMDLYNGEIVAYELSTRPCFELVTSMLDKALQQLQDEPKLVMHSDQGWQYQHAQYRQKLAAKGVKQSMSRKGNCLDNAAMESFFGTL
KSEFFYLKRFESIEELTAGLEEYIRYYNHDRIKLKLGGLSPVKYRTQAAS

 

Blast result :
Comments
There are 5 copies of ISPsy8 in the chromosome of this strain.
References