ISPsy5

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE016853 ND Pseudomonas syringae
Pseudomonas syringae DC3000
DNA section
IS Length : 2059 bp

Ends


IR Length : 21/28

IRL : GTATCCGATCCACGAACCCCACATTTCAAGCCACTGGCTGACGCCTGATG
IRR : GTAAGCGATCCACCAACCACACCTACCATCAGATGCGGCTTCCGTTTATC

Insertion site


Left flankDirect repeatRight flankDR Length
CATGCGGTCTGTTTTAATGAATCCGCAA8
ACGGTTCAGGAATTGAATTGGCTGCTGG8
CTGAATCGGCGTAGTCTCGCCCTTTACC8
CCTTGATGCGGTTGGAACGACCCCAGCC8
GCAAATGTCTGAAGATTCTGCATGATAT8
CGGCAAACCCGATACGCTTGAAAATGAC8
GACTAAGGCAGTTGCTTCTGCCATGGCA8
GAACTTTGTGATCGTGCCACAGGCTGAC8
GGGAAGTAGTGATAGAACGACCCTTTGG8
TCGACACCCTGATTCATCACTCCCGGCG8
TCCGAACTCGAATATACCGGCAAGAAAA8
GATGGTGAGTGTCCATTACATCACGACA8
GGGAGACACCAAAACAACACGCCTACGG8
TTAGCCTATTTTTCTTTTTTAGGTTTTG8
CTTACGCCTCACTCAACCCACGCCAGAA8
GACCAGTACAAATATTGCGCAGTGCTGG8
TAGCGGATCTGGTGCTTCAGATCGAAGA8
CACCGGGGCTGACTCACGACCGTGCTCA8
TCAGGCGGTGCATTCACCAACGCCAAAT8
ACGATAGTTGAGATTATCGTTCCTCAGC8
ACCCGCAGCTGGACCAAAGTCAAGTCGG8
AGCTTTGTCCGTTTCTCCGAGCGAGAGG8
GGGAACTGTCAATTAATTAGTCAGGATT8
CTATTTATCTGGTTATAGCGCGAGGACT8
GGATGGATTTGATCAAGCTGCTGC4
AGTAACCATCAGTTATCCAC0
CAGATCAAGCGTTAAAGAGC0
GGCATCGAACATTTCAGCGC0
GTGCTCTTCGTTTCTAGTAG0
CATATTCAATGACACAAATA0
CTTTCCATTTGTTCTTCCGG0
CTGGAGCGCCAAAAGCTCAC0
CCAATTCAATCGGGAATTGC0

DNA sequence

GTATCCGATCCACGAACCCCACATTTCAAGCCACTGGCTGACGCCTGATGCTGTGCTCCCGTTTTCTTTCAGGAGCCAGCACCATGATGCGACCCGACGC
CAAAGTCGAGAAAGTCTACCTCTATCCCAAACCCGTGGATTTCAGAAAATCCATCGATGGCTTAGCTGCTTTGGTCGAGTTGGATATTAAAGTGGCGGTC
TTTGATCCGGTGCTCTTTGTCTTTCTCAACAAATCCCGGAATCGAGTGAAGATTTTGTATTGGGAGCGTAACGGCTTCTGCCTTTGGCTCAAGCGTCTCG
AGTCCGAGCGTTTCAAAATCTCGCCAGATGATGTCGACGAAGCCATTGTGCTGACGGTTCAGGAATTGAATTGGCTGCTGGATGGATTTGATCTGTGGCG
CAACCGTCCTCATAAGATTTTGACTCCAAGGTTTGTTGCCTGACGGGTATAATCCAGGGCATGCGACCATTGCCCGACAACCTTCCTGACGATCCCGTAT
TGCTCAAACAGATGCTGCTTGAGAGCTACGAAGCACGCTCGCGCGAACAGGAAGTTAAAGACGCCTACGCCACTCACATCGTTGATCTCAAAGAGCAGAT
CAAGCTGCTGCGTGACCGTATTTTTGGTCGAAAGTCCGAACAAACCGTCGAGCCTAATACCCCTCAACTTGCCTTGTTCAATGAACCTGAAAGCGAGCCT
ATGCTCCCGGTCGGCGATGCTGATGAGGAAGTAGTTGCTCCTGCGCCACGCCGTGGCAAGCGCAAGCCACTGTCGGCTGATCTGCCGCGCATTGAAGTCA
TCCACGAGCTGCCCGAACACGAACTGACCTGTGCCTGCGGTTGCCGCAAGCATGTCGTCAGCGAGGAAACCAGCGAGCAACTGGATATCGTGCCGATGCA
GATTCGGGTGATCAAACACATCCGCAAAGTCTACGGCTGTCGTGGCTGTGAGTCGGCACCGGTCACAGCCGGCAAGCCTGCGCAGTTGATTGAAAAGAGC
ATGGCCAGCCCCAGCGTGTTGGCCATGCTGCTGACCACCAAGTATGTCGACGGCTTGCCGCTGCATCGTTTTGAAACGGTGCTAAGCCGACATGGCATAG
AAATCCCGCGTCAAACGTTGGCCCGCTGGATTATCCAGTGCGGCGAACATTTGCAGCCGCTGTTAAATCTGATGCGTGATCGATTGCTGGAAAGCCCGGT
CATCCACTGTGACGAAACCCGCGTTCAGGTACTGAAAGAACCAGATCGAGACCCGACCAGCCAATCCTGGATGTGGGTACAGGCCAGCGGGCCTCCAGAT
CATAAAGTCGTATTGTTCGACTACACTTCCAGCCGCGCGCAGGACGTACCGTTACGGCTGCTGGAAAGTTATCGCGGCTATGTGATGACTGATGATTACG
CGGGCTATAACGCGCTGGCGTTACAACCGGGCGTGGAGCGCTTGGCGTGTATGGCCCATGCGCGGCGCAAATTTGTCGAAGCACAAAAAGTGCAACCTAA
GGGCAAGACCGGGCGTGCCGATATCGCGCTGACGATGATCAATAAGCTGTACGGTATCGAGCGCGAATTGAAAGACGGCAGCGACGAACAGCGCTTTACC
GGTCGGCAGGAGAAAAGCCTGCCAATCTTGGCTCAGTTGAAAAGCTGGCTGGATAAAACTCAGTCCCACGTGACGCCGCAAAGCGTGCTGGGCAAGGCAG
TTGGTTATCTGGCGAATAACTGGAGTCGGCTGAAGCGTTACGTGGAAGCCGGCTTTTTACCGATCGACAACAACCCGGCAGAGCGCGCAATAAAGCCGTT
TGTTATTGGGCGCAAAGCCTGGCTGTTCAGCGACACGCCTAAAGGCGCCACCGCCAGTGCACAGATCTATAGCTTGGTCGAGACCGCCAAGGTCAACGGC
CAGGAGCCCTATACGTGGCTGCGCCACGTACTGGAGAGGTTGCCACTTGCGCAGTCGGTGGCCGACTATGAGGCGTTGCTGCCGTGGAACTGCTCACCGG
AAATGCCAAGATAAACGGAAGCCGCATCTGATGGTAGGTGTGGTTGGTGGATCGCTTAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
360 bp119 aa84443+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MMRPDAKVEKVYLYPKPVDFRKSIDGLAALVELDIKVAVFDPVLFVFLNKSRNRVKILYWERNGFCLWLKRLESERFKISPDDVDEAIVLTVQELNWLLD
GFDLWRNRPHKILTPRFVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1554 bp517 aa4612014+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRPLPDNLPDDPVLLKQMLLESYEARSREQEVKDAYATHIVDLKEQIKLLRDRIFGRKSEQTVEPNTPQLALFNEPESEPMLPVGDADEEVVAPAPRRGK
RKPLSADLPRIEVIHELPEHELTCACGCRKHVVSEETSEQLDIVPMQIRVIKHIRKVYGCRGCESAPVTAGKPAQLIEKSMASPSVLAMLLTTKYVDGLP
LHRFETVLSRHGIEIPRQTLARWIIQCGEHLQPLLNLMRDRLLESPVIHCDETRVQVLKEPDRDPTSQSWMWVQASGPPDHKVVLFDYTSSRAQDVPLRL
LESYRGYVMTDDYAGYNALALQPGVERLACMAHARRKFVEAQKVQPKGKTGRADIALTMINKLYGIERELKDGSDEQRFTGRQEKSLPILAQLKSWLDKT
QSHVTPQSVLGKAVGYLANNWSRLKRYVEAGFLPIDNNPAERAIKPFVIGRKAWLFSDTPKGATASAQIYSLVETAKVNGQEPYTWLRHVLERLPLAQSV
ADYEALLPWNCSPEMPR

 

Blast result :
Comments
There are 33 intact copies and 13 incomplete copies of ISPsy5 in the chromosome of this strain, and 25 of these have direct repeats outside the insertion sequence. There are no copies in the two plasmids.
References
1] Buell,C.R., Joardar,V., Lindeberg,M., Selengut,J., Paulsen,I.T.,Gwinn,M.L., Dodson,R.J., Deboy,R.T., Durkin,A.S., Kolonay,J.F., Madupu,R., Daugherty,S., Brinkac,L., Beanan,M.J., Haft,D.H., Nelson,W.C., Davidsen,T., Zafar,N., Zhou,L., Liu,J., Yuan,Q., Khouri,H., Fedorova,N., Tran,B., Russell,D., Berry,K.,Utterback,T., Van Aken,S.E., Feldblyum,T.V., D'Ascenzo,M., Deng,W.L., Ramos,A.R., Alfano,J.R., Cartinhour,S., Chatterjee,A.K., Delaney,T.P., Lazarowitz,S.G., Martin,G.B., Schneider,D.J., Tang,X., Bender,C.L., White,O., Fraser,C.M. and Collmer,A. (2003) PNAS, 100(18),10181-10186.