ISFlsp1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AJ421424 ND Flavobacterium sp
Flavobacterium sp ATCC 27551 plasmid pPDL2
DNA section
IS Length : 2625 bp

Ends


IR Length : 13/16

IRL : AACGCCGGAGTAAAAACATACCACTGACGGGTCTTTCGGGGTCCGTTCAT
IRR : TAATGCCGGGGTAAAATTGTGCAAGATTCCGGTGTAAGAGTGTACCTAGT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

AACGCCGGAGTAAAAACATACCACTGACGGGTCTTTCGGGGTCCGTTCATTTCGGAGTAAAAAGTGTACCGGTCCTGATGGGTCCTCTGGAGAGAGGGCC
GGAGGATGAAGTCAGTGGAGAATATATGCGAAGGTCCGTCGGGCCGTATTGGTTGAAGGGATGACGCGGCGAGAAGCAGCGCAGTTATTTTGGTGTTCAT
CGCAACACGATCACGAAGATGCTGCAGTATGCGGAGCCACCGGAGATATCGGCGAGCGGTGCCGCGGGTGTCGGAGAAGCTGGCACCGTTCGAGACGTTA
ATCGAACGAGATATTACGTTCGGACAAAGGTGCTCCTCCCAAGCAGCGCCATACGTGCAAGCGGAATATACGAACGGCTGCGGACTGAGCATGGGTATAC
GGGCGGTCTGACGATCCTGAGCGATTAATGTTCGTTCGCAGCGGTTGCGTTCCCGCGAGGTATTCATACCGCTGAGCCACAGGCCTGAGGCACGCTCAGG
TGGATTTTGGCGAAGCCGATGCTATCATCGCAGGCAAGCGGGTGCGGCATGCATTATTTCTGCATGGATTTGCCACAGTCCGATGGGTGTTTTGTGAAGG
CCTATCCCGACCGAAGTGGCGGAAGCGTTTTGCGATGGCCATGTCAGCGCCTTTGCCTTTTTCGGCGGTGATGCCTACGCGCATCCTGTACGATAACACC
CGGCTTGCGGTGGCGCGCATTCTTGGCGATGAGACGGCGCGAACGCAGCCGGATGTTTGCGGGCTTGCAGAGCCATTATCTGTTCGACGATCAGGTTCGG
TCGCCCCGGCAAGGGGAACGACAAGGGCAAGGTCGAGGGACTGGTCGGCTATGATGCGCCGCAACTTCATGGTGCCGATACCGGCGGCGGCGAGTATCGA
AGAACTGAACGCCCAGCTTTGCCGATCAATGCCGGCGTCGCGGTGCAGCGGTATTGCGGGGACAAAGCCAGAGCAATCACGGCGCGCATGGAAGCCGATA
GTGCCGCGTTCATGCCCTTGCCGGAGGTGGCGTTCGAATCCGTGCCATATCGACAGCGGCTGCGCGTCGTCGATGGCGCTGGTGCGCTATCGCACCAAAC
GATTACTCGGTACCCACGGCGTTCGCGCATCAGCAGGTCGTCATCAAGGGCTATGTCGAATCGTGTCGACATCGTCTGTCGGGGCACGTGCATCGCCAGC
CATGTGCGCAGGTATGAGCAGAGAGGATTTTATTGCCAACCCGCTCCACTATCTGGCCCTGCTCGAGCATAAGCCCGGCGACACTGGATCAGGCCGCGCC
GCTTGATGGCTGGCACCTGTCCGAACCGGTGCACCGCCTGCAGGCGGCTGATGGAAGCGAGAAGCGGCAAGGAAGGACGGCGTGCCTTCATCCAGGTCCT
GCAGGTTGTGCGAACATTATGAGCAGTCGCTGGTCGAGTGGGCGGTCGCCCGTGCGCTGGAGCATGGGCGCGATCAGCTTTGATGCGGTCAAGATGATCC
TGCTTGCGCGTCTGGAACACCGACACCGCGAGGCTGGATATGAGCCTGTATCCCTATCTGCCCCGCGCCAATGTCGGTGTGACCGAATACCCGCGCCTAT
CTGGGCCTGATACCCGATGCCCACCGCGTGACAATGAAGGGGGCATACAGCATGACAGACCCTGTGATGCAACCGCTGCCTGCCATAATGGAGACGGGCA
CCAGCGTAGCCGCCGCAGATCCTGCTGGGCAACCACTTGAAAGCGCTCAAGCTGCCGACCTTCGCTCGACGAGTATGAGAAGGTGGCGATGGAAGCGGCG
GGCAGCCGCACCGATTATCCCGGCTATCTAGCTGCGCCTGTGCGAGTTGGAACGGATCGACCGGGAGCGGCGCATGGTCGAACGCCGTATACCGGCTCGC
CAGGTTCCCGCACACCAAGAGCTTCGATACGTTCGATTTTGCCGCGCAGCCATTCGCTGAACAAGCCGCTGGTGGTTGAGCTTGCGCGATGCGAATGGAT
TACGACGCGCAGACAACATTATCGCCCTTGGGCCCTCCGGCACCGGCAAGACCCATGTCGCGCTCGCGCTCGGACCTGGCGGCCTGTCAGAAAGGACACA
GCGTCGCCTTCGTTACAGCCGCCGCCCTCGTGCAATGAGCTGATGGAGGCAAGAGACGAGCGCCGGTTGCGCCAGCTGCAAAAGCATCTCGCCTCAAGTC
AAGCTGCTGATCCTTGATGAACTGGGTTATGTCCCGTTCACCGCCGTCGGCAGTGAAACTGCTGTTCGAGGTGCTCAGCCAGCGCTACGAACGCGGCAGC
ACGCTCATCACCAGCAAATCTGCCGTTCGATGAATGGACATCGGTGTTCGGATCCGAGCGGCTTACCGGCGCCCTGCTACGACCGGTTGACCCACCATGT
CCATATCCTCGAGATGAACGGGGGCAGCTATCGGCTTGCACAGCAGCCGGAAACGGCAAAAGCACAGCCACCACGCACAGCAAGAGGAGGTGCCCCCCCC
AATGACCTGATCAGAAAACCCCTCATCTGCTGTGCTGAACGGCCTTCCGCTACGCTCCAGAACCGTTCAGCACAGCAGATGAAAGCACCGCCTCGACAAG
AGGCTTTTTGTTCAATCCAACTAGGTACACTCTTACACCGGAATCTTGCACAATTTTACCCCGGCATTA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1524 bp507 aa1051628+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSVEIYAKVRRAVLVEGMTRREAARYFGVHRNTITKMLQYAEPPGYRRAVPRVSEKLAPFETLIDEILRSDKGAPPKQRHTCKRIYERLRTEHGYTGGL
TILSDYVRSQRLRSREVFIPLSHRPGHAQVDFGEADAIIAGKRVRLHYFCMDLPQSDGCFVKAYPAEVAEAFCDGHVSAFAFFGGVPTRILYDNTRLAVA
RILGDGRRERSRMFAGLQSHYLFDDRFGRPGKGNDKGKVEGLVGYVRRNFMVPIPAAASIEELNARFADQCRRRGAAVLRGQSQSITARMEADSAAFMPL
PEVAFDPCHIDSGCASSMALVRYRTNDYSVPTAFAHQQVVIKGYVDRVDIVCRGTCIASHVRRYEREDFIANPLHYLALLEHKPGALDQAAPLDGWHLSE
PVHRLRRLMEARSGKEGRRAFIQVLRLCEHYEQSLVEWAVARALELGAISFDAVKMILLARLEHRPARLDMSLYPYLPRANVGVTDTRAYLGLIPDAHRV
TMKGASA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
840 bp279 aa16252464+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTDPVMQPLPAIMETGTSVPPQILLGNHLKALKLPTFAREYEKVAMEAAGSRTDYPGYLLRLCELERIDRERRMVERRIRLARFPHTKSFDTFDFAAQPS
LNKPLVVELARCEWITTRSNIIALGPSGTGKTHVALALGLAACQKGHSVAFVTAAALVHELMEARDERRLRQLQKHLASVKLLILDELGYVPFTAVGSEL
LFEVLSQRYERGSTLITSNLPFDEWTSVFGSERLTGALLDRLTHHVHILEMNGGSYRLASSRKRQKHSHHAQQEEVPPP

 

Blast result :
Comments
ISFlsp1 is adjacent to the structural gene for parathion hydrolase (opd) and is potentially part of a complex catabolic transposon.
The IstA and IstB proteins are most similar to those encoded by an insertion sequence within the genome of Agrobacterium tumefaciens strain C58.
References
1] Merrick M. (2001) Direct submission