ISPpu15

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002947 ND Pseudomonas putida
Pseudomonas putida KT2440
DNA section
IS Length : 2041 bp

Ends


IR Length : 22/28

IRL : GTAAGGGATCCATCATCCCCACATTCCAACCGTCCACCTGATTCCCGATG
IRR : GTAACCGATCCATGAACCCCACCTACCACAAGGTGGAAGCCAAAGTTACC

Insertion site


Left flankDirect repeatRight flankDR Length
GGCTGGGGTTTTCTAAGCCATGTTAGCT8
CAGGTGGACTATATCGTTGGCCTGTGCA8
CGGCCAGAGCCGACCGCTGTACTCCACC8

DNA sequence

GTAAGGGATCCATCATCCCCACATTCCAACCGTCCACCTGATTCCCGATGCTGTGCTCCCGATTTCTTTCTGGAGCCAGCCCGCCATGATGCGCCCCGAC
AGCAAAGTCGAAAAAGTCTACCTCTACCCCAAGCCCGTGGACTTTCGAAAGTCCATCGACGGCCTCACTGCCCTGGTCGAACTGGACATCAAGGTCGCCG
TGTTCGACCCGGTACTTTTCGTCTTCCTCAATAAAGCCCGTAACCGCATCAAGGTGTTGTATTGGGAGCGCAACGGCTTCTGCCTTTGGCTCAAGCGCCT
CGAAGCCGAGCGTTTCAAAACTTCACCCGACGCGACCGACGAAGCTATCGTACTGACCGTCCAGGAACTGAATTGGCTACTCGACGGTTTCGATCTCTGG
CGCAACCGTCCGCATCAGGTTTTGACTCCTCGTTTCGTCGCCTGACCGGGTATAATCCGGGCCATGATTTCAGTGCCCGATAACCTTCCTGACGACCCTG
CCGCGCTCAAACTACTGCTTGCGCAGTTGTTGGCCGAGCGCACGGTCGACAAAGGTCAGATCGTCGACCTTAAAGAACAGGTCAAGCTGCTGCGTGACCG
ATTATTCAATCGCAAGTCCGAGCAAACCGTCGAGTCCAACACGCCCCAACTGGCGTTGTTCAATGAACCCGAAAGTCAGTGGGTACCTACCGCTGATGAT
GCCGACGAAGAAGTTGTTGCGCCAAGCAAGCAGCGCGGCAAACGCAAGCCACTGTCGGCTGACCTGCCACGCATTGAAGTCATTCATGACTTGCCCGAGC
ACGAACTGACTTGCGCCTGTGGTTGCCGCAAACACGTGGTTGGCGAGCAAACCAGCGAACAACTCGACATCGTGCCGATGCAGATCCGTGTGCTTAAACA
CGTACGCAAAATCTATGGCTGCCGTGGCTGCGAAACCGCGCCGGTCACCGCTGACAAGCCGGCCCAATTGATTGAAAAGAGCATGGCCAGTCCCAGCGTG
CTGGCGATGTTGCTAACCACCAAGTACGTCGATGGCCTGCCGCTGCACCGCTTCGAAACGGTGCTGAACCGACATGGTATCGAGATCCCCCGGCAAACCC
TGGCCCGCTGGGTCATCCAGTGCAGCGAACACTTTCAGCCACTGCTGAACCTGATGCGTGACCGCCTGCTGGAAAGCCCGGTGATCCATTGCGATGAAAC
TCGCGTGCAGGTGCTCAAAGAGCCGGATCGTGATCCGACCAGTCAATCCTGGATGTGGGTGCAGGCCAGTGGCCCGCCTGATCGACAAGTCGTGCTGTTC
GATTACACCACCAGCCGCGCGCAGGAGGTGCCGCTGCGCCTGCTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGG
CGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGCGGTCGCGC
CGATGTTGCGCTGACGATGATCAACAAACTGTATGGCATCGAACGCGACCTCAAGGATGCCAGCGATGAACAGCGATTCATCGGTCGTCAGGAACGAAGC
CTGCCGATCCTTGAGCAGTTGAAAAGTTGGCTCGACAAGACGCACTCACAGGTGACACCGCAAAGCGTACTGGGCAAGGCGGTGCTTTACCTGGCAAACA
ACTGGAACCGCCTGGAGCGGTATGTCGAGGCGGGCCACCTGCCGATCGACAACAACCTGGCAGAGCGGGCCATAAAGCCGTTTGTGATCGGGCGCAAAGC
TTGGCTGTTCAGTGACACGCCCAAGGGAGCCACGGCTAGCGCGCAGATCTACAGCTTGGTCGAAACCGCCAAGGTCAACGGCCAAGAGCCCTATACGTGG
CTGCGCCACGTCCTGGAGCGACTGCCGCACGCCTCATCGGTAGCAGACTACGAAGCGCTGCTGCCATGGAACTGTTCGCCAGAGATGCCACGGTAACTTT
GGCTTCCACCTTGTGGTAGGTGGGGTTCATGGATCGGTTAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
360 bp119 aa86445+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MMRPDSKVEKVYLYPKPVDFRKSIDGLTALVELDIKVAVFDPVLFVFLNKARNRIKVLYWERNGFCLWLKRLEAERFKTSPDATDEAIVLTVQELNWLLD
GFDLWRNRPHQVLTPRFVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1533 bp510 aa4641996+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISVPDNLPDDPAALKLLLAQLLAERTVDKGQIVDLKEQVKLLRDRLFNRKSEQTVESNTPQLALFNEPESQWVPTADDADEEVVAPSKQRGKRKPLSAD
LPRIEVIHDLPEHELTCACGCRKHVVGEQTSEQLDIVPMQIRVLKHVRKIYGCRGCETAPVTADKPAQLIEKSMASPSVLAMLLTTKYVDGLPLHRFETV
LNRHGIEIPRQTLARWVIQCSEHFQPLLNLMRDRLLESPVIHCDETRVQVLKEPDRDPTSQSWMWVQASGPPDRQVVLFDYTTSRAQEVPLRLLEGYRGY
VMTDDYAGYNALALQPGVERLACMAHVRRKFVEAQKVQPKGKSGRADVALTMINKLYGIERDLKDASDEQRFIGRQERSLPILEQLKSWLDKTHSQVTPQ
SVLGKAVLYLANNWNRLERYVEAGHLPIDNNLAERAIKPFVIGRKAWLFSDTPKGATASAQIYSLVETAKVNGQEPYTWLRHVLERLPHASSVADYEALL
PWNCSPEMPR

 

Blast result :
Comments
There are 4 copies of ISPpu15 in this strain, and one copy lacks a target site duplication. Three copies of ISPpu15 disrupt cellular genes.
ISPpu15 is 68% aa (ORF1) similar to IS1326 and 52% (ORF2) to ISRm14 (ORF3).
References
1] DeBoy., R. (2002) Direct submission