ISPpu15
- Family IS66
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_002947 | ND | Pseudomonas putida | Pseudomonas putida KT2440 |
DNA section
IS Length : 2041 bp
Ends
IR Length : 22/28
IRL : GTAAGGGATCCATCATCCCCACATTCCAACCGTCCACCTGATTCCCGATG
IRR : GTAACCGATCCATGAACCCCACCTACCACAAGGTGGAAGCCAAAGTTACC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGCTGGGGTT | TTCTAAGC | CATGTTAGCT | 8 |
CAGGTGGACT | ATATCGTT | GGCCTGTGCA | 8 |
CGGCCAGAGC | CGACCGCT | GTACTCCACC | 8 |
DNA sequence
GTAAGGGATCCATCATCCCCACATTCCAACCGTCCACCTGATTCCCGATGCTGTGCTCCCGATTTCTTTCTGGAGCCAGCCCGCCATGATGCGCCCCGAC
AGCAAAGTCGAAAAAGTCTACCTCTACCCCAAGCCCGTGGACTTTCGAAAGTCCATCGACGGCCTCACTGCCCTGGTCGAACTGGACATCAAGGTCGCCG
TGTTCGACCCGGTACTTTTCGTCTTCCTCAATAAAGCCCGTAACCGCATCAAGGTGTTGTATTGGGAGCGCAACGGCTTCTGCCTTTGGCTCAAGCGCCT
CGAAGCCGAGCGTTTCAAAACTTCACCCGACGCGACCGACGAAGCTATCGTACTGACCGTCCAGGAACTGAATTGGCTACTCGACGGTTTCGATCTCTGG
CGCAACCGTCCGCATCAGGTTTTGACTCCTCGTTTCGTCGCCTGACCGGGTATAATCCGGGCCATGATTTCAGTGCCCGATAACCTTCCTGACGACCCTG
CCGCGCTCAAACTACTGCTTGCGCAGTTGTTGGCCGAGCGCACGGTCGACAAAGGTCAGATCGTCGACCTTAAAGAACAGGTCAAGCTGCTGCGTGACCG
ATTATTCAATCGCAAGTCCGAGCAAACCGTCGAGTCCAACACGCCCCAACTGGCGTTGTTCAATGAACCCGAAAGTCAGTGGGTACCTACCGCTGATGAT
GCCGACGAAGAAGTTGTTGCGCCAAGCAAGCAGCGCGGCAAACGCAAGCCACTGTCGGCTGACCTGCCACGCATTGAAGTCATTCATGACTTGCCCGAGC
ACGAACTGACTTGCGCCTGTGGTTGCCGCAAACACGTGGTTGGCGAGCAAACCAGCGAACAACTCGACATCGTGCCGATGCAGATCCGTGTGCTTAAACA
CGTACGCAAAATCTATGGCTGCCGTGGCTGCGAAACCGCGCCGGTCACCGCTGACAAGCCGGCCCAATTGATTGAAAAGAGCATGGCCAGTCCCAGCGTG
CTGGCGATGTTGCTAACCACCAAGTACGTCGATGGCCTGCCGCTGCACCGCTTCGAAACGGTGCTGAACCGACATGGTATCGAGATCCCCCGGCAAACCC
TGGCCCGCTGGGTCATCCAGTGCAGCGAACACTTTCAGCCACTGCTGAACCTGATGCGTGACCGCCTGCTGGAAAGCCCGGTGATCCATTGCGATGAAAC
TCGCGTGCAGGTGCTCAAAGAGCCGGATCGTGATCCGACCAGTCAATCCTGGATGTGGGTGCAGGCCAGTGGCCCGCCTGATCGACAAGTCGTGCTGTTC
GATTACACCACCAGCCGCGCGCAGGAGGTGCCGCTGCGCCTGCTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGG
CGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGCGGTCGCGC
CGATGTTGCGCTGACGATGATCAACAAACTGTATGGCATCGAACGCGACCTCAAGGATGCCAGCGATGAACAGCGATTCATCGGTCGTCAGGAACGAAGC
CTGCCGATCCTTGAGCAGTTGAAAAGTTGGCTCGACAAGACGCACTCACAGGTGACACCGCAAAGCGTACTGGGCAAGGCGGTGCTTTACCTGGCAAACA
ACTGGAACCGCCTGGAGCGGTATGTCGAGGCGGGCCACCTGCCGATCGACAACAACCTGGCAGAGCGGGCCATAAAGCCGTTTGTGATCGGGCGCAAAGC
TTGGCTGTTCAGTGACACGCCCAAGGGAGCCACGGCTAGCGCGCAGATCTACAGCTTGGTCGAAACCGCCAAGGTCAACGGCCAAGAGCCCTATACGTGG
CTGCGCCACGTCCTGGAGCGACTGCCGCACGCCTCATCGGTAGCAGACTACGAAGCGCTGCTGCCATGGAACTGTTCGCCAGAGATGCCACGGTAACTTT
GGCTTCCACCTTGTGGTAGGTGGGGTTCATGGATCGGTTAC
AGCAAAGTCGAAAAAGTCTACCTCTACCCCAAGCCCGTGGACTTTCGAAAGTCCATCGACGGCCTCACTGCCCTGGTCGAACTGGACATCAAGGTCGCCG
TGTTCGACCCGGTACTTTTCGTCTTCCTCAATAAAGCCCGTAACCGCATCAAGGTGTTGTATTGGGAGCGCAACGGCTTCTGCCTTTGGCTCAAGCGCCT
CGAAGCCGAGCGTTTCAAAACTTCACCCGACGCGACCGACGAAGCTATCGTACTGACCGTCCAGGAACTGAATTGGCTACTCGACGGTTTCGATCTCTGG
CGCAACCGTCCGCATCAGGTTTTGACTCCTCGTTTCGTCGCCTGACCGGGTATAATCCGGGCCATGATTTCAGTGCCCGATAACCTTCCTGACGACCCTG
CCGCGCTCAAACTACTGCTTGCGCAGTTGTTGGCCGAGCGCACGGTCGACAAAGGTCAGATCGTCGACCTTAAAGAACAGGTCAAGCTGCTGCGTGACCG
ATTATTCAATCGCAAGTCCGAGCAAACCGTCGAGTCCAACACGCCCCAACTGGCGTTGTTCAATGAACCCGAAAGTCAGTGGGTACCTACCGCTGATGAT
GCCGACGAAGAAGTTGTTGCGCCAAGCAAGCAGCGCGGCAAACGCAAGCCACTGTCGGCTGACCTGCCACGCATTGAAGTCATTCATGACTTGCCCGAGC
ACGAACTGACTTGCGCCTGTGGTTGCCGCAAACACGTGGTTGGCGAGCAAACCAGCGAACAACTCGACATCGTGCCGATGCAGATCCGTGTGCTTAAACA
CGTACGCAAAATCTATGGCTGCCGTGGCTGCGAAACCGCGCCGGTCACCGCTGACAAGCCGGCCCAATTGATTGAAAAGAGCATGGCCAGTCCCAGCGTG
CTGGCGATGTTGCTAACCACCAAGTACGTCGATGGCCTGCCGCTGCACCGCTTCGAAACGGTGCTGAACCGACATGGTATCGAGATCCCCCGGCAAACCC
TGGCCCGCTGGGTCATCCAGTGCAGCGAACACTTTCAGCCACTGCTGAACCTGATGCGTGACCGCCTGCTGGAAAGCCCGGTGATCCATTGCGATGAAAC
TCGCGTGCAGGTGCTCAAAGAGCCGGATCGTGATCCGACCAGTCAATCCTGGATGTGGGTGCAGGCCAGTGGCCCGCCTGATCGACAAGTCGTGCTGTTC
GATTACACCACCAGCCGCGCGCAGGAGGTGCCGCTGCGCCTGCTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGG
CGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGCGGTCGCGC
CGATGTTGCGCTGACGATGATCAACAAACTGTATGGCATCGAACGCGACCTCAAGGATGCCAGCGATGAACAGCGATTCATCGGTCGTCAGGAACGAAGC
CTGCCGATCCTTGAGCAGTTGAAAAGTTGGCTCGACAAGACGCACTCACAGGTGACACCGCAAAGCGTACTGGGCAAGGCGGTGCTTTACCTGGCAAACA
ACTGGAACCGCCTGGAGCGGTATGTCGAGGCGGGCCACCTGCCGATCGACAACAACCTGGCAGAGCGGGCCATAAAGCCGTTTGTGATCGGGCGCAAAGC
TTGGCTGTTCAGTGACACGCCCAAGGGAGCCACGGCTAGCGCGCAGATCTACAGCTTGGTCGAAACCGCCAAGGTCAACGGCCAAGAGCCCTATACGTGG
CTGCGCCACGTCCTGGAGCGACTGCCGCACGCCTCATCGGTAGCAGACTACGAAGCGCTGCTGCCATGGAACTGTTCGCCAGAGATGCCACGGTAACTTT
GGCTTCCACCTTGTGGTAGGTGGGGTTCATGGATCGGTTAC
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
360 bp | 119 aa | 86 | 445 | + | No |
AG : IS66 TnpB
ORF sequence :
MMRPDSKVEKVYLYPKPVDFRKSIDGLTALVELDIKVAVFDPVLFVFLNKARNRIKVLYWERNGFCLWLKRLEAERFKTSPDATDEAIVLTVQELNWLLD
GFDLWRNRPHQVLTPRFVA
GFDLWRNRPHQVLTPRFVA
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1533 bp | 510 aa | 464 | 1996 | + | No |
Chemistry : DDE
ORF sequence :
MISVPDNLPDDPAALKLLLAQLLAERTVDKGQIVDLKEQVKLLRDRLFNRKSEQTVESNTPQLALFNEPESQWVPTADDADEEVVAPSKQRGKRKPLSAD
LPRIEVIHDLPEHELTCACGCRKHVVGEQTSEQLDIVPMQIRVLKHVRKIYGCRGCETAPVTADKPAQLIEKSMASPSVLAMLLTTKYVDGLPLHRFETV
LNRHGIEIPRQTLARWVIQCSEHFQPLLNLMRDRLLESPVIHCDETRVQVLKEPDRDPTSQSWMWVQASGPPDRQVVLFDYTTSRAQEVPLRLLEGYRGY
VMTDDYAGYNALALQPGVERLACMAHVRRKFVEAQKVQPKGKSGRADVALTMINKLYGIERDLKDASDEQRFIGRQERSLPILEQLKSWLDKTHSQVTPQ
SVLGKAVLYLANNWNRLERYVEAGHLPIDNNLAERAIKPFVIGRKAWLFSDTPKGATASAQIYSLVETAKVNGQEPYTWLRHVLERLPHASSVADYEALL
PWNCSPEMPR
LPRIEVIHDLPEHELTCACGCRKHVVGEQTSEQLDIVPMQIRVLKHVRKIYGCRGCETAPVTADKPAQLIEKSMASPSVLAMLLTTKYVDGLPLHRFETV
LNRHGIEIPRQTLARWVIQCSEHFQPLLNLMRDRLLESPVIHCDETRVQVLKEPDRDPTSQSWMWVQASGPPDRQVVLFDYTTSRAQEVPLRLLEGYRGY
VMTDDYAGYNALALQPGVERLACMAHVRRKFVEAQKVQPKGKSGRADVALTMINKLYGIERDLKDASDEQRFIGRQERSLPILEQLKSWLDKTHSQVTPQ
SVLGKAVLYLANNWNRLERYVEAGHLPIDNNLAERAIKPFVIGRKAWLFSDTPKGATASAQIYSLVETAKVNGQEPYTWLRHVLERLPHASSVADYEALL
PWNCSPEMPR
Blast result :
Comments
There are 4 copies of ISPpu15 in this strain, and one copy lacks a target site duplication. Three copies of ISPpu15 disrupt cellular genes.
ISPpu15 is 68% aa (ORF1) similar to IS1326 and 52% (ORF2) to ISRm14 (ORF3).
ISPpu15 is 68% aa (ORF1) similar to IS1326 and 52% (ORF2) to ISRm14 (ORF3).
References
1] DeBoy., R. (2002) Direct submission