ISPpu14
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002947 | ND | Pseudomonas putida | Pseudomonas putida KT2440 |
DNA section
IS Length : 2383 bp
Ends
IR Length : 27/33
IRL : GTAAGCGTCCGGGGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGG
IRR : GTAAGCGTCCGCCCAACGCACCTTGCGTAAGTCAGGCCGATACCCACTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCGAACGCA | TACAGCGA | GGCCATTTCG | 8 |
| TTGTCCGAGG | AAGCAAGTGA | 0 | |
| TGCAACCGGG | TTCCTATG | TTCAAAAAAG | 8 |
| GACAGTGGGC | TACATGGC | CCCCTCGCTG | 8 |
| TCGCCAGGAA | TCACCATC | GGCCTGCGTG | 8 |
| CATGATTCCA | GCCGCTTC | CACTAATCCG | 8 |
DNA sequence
GTAAGCGTCCGGGGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATTGCCAGGATTTCC
ATGCGCCAACGAAGCTCTTATCCGAAACCGTTCAAAGCCCAGGTCGTTCAGGAATGCCTGCAACCTGGCGCAACCGTGTCCAGTGTCGCCATCAGCCACG
GCATCAATGCCAACGTCATCCGCAAATGGCTGCCGCTTTATCGAGATCAGCCTGCAGCAGCTTCAATACCGGCATTCGTCCCACTAAAAACCGCGCCTAA
ACGGCCAGTCGAAGCGTCAGTGATCATCGAGTTGCCTATGACCGGGCAAGTGATCACGGTGAAATGGCCAACCTCAGATCCTGAGGGCTGCGCGCAATTT
ATCCGGGCTGTCGCTCAATGATCCGCATCGATGCGATCTGGCTGGCCACCGAGCCGATGGATATGCGCGCCGGCACCGAAACGGCATTGGCCCGGGTGAT
CGCGGTGTTCGGTGCGGCGAAGCCGCACTGCGCTTATCTGTTCGCCAATCGCCGGGCCAACCGGATGAAGGTGCTGGTGCACGACGGCGTGGGCGTCTGG
CTTGCAGCGCGACGACTGAATCAAGGCAAGTTTCACTGGCCCGGCATTCGCCATGGCAGCGAGGTCGAACTCGACAGCGAACAACTCCAGGCGTTGGTAC
TGGGTTTGCCATGGCTGCGCGTTGGTGCAGGCGGTGTGATCAGCATGCTGTAATCGCCGGCCATTTGACCGGTCTGTTTGTCGTCGTTTCGGGCCTGCTC
TGGCACAATCGGCGGCATGACTTTCTCGCCCAATCTCGACCAGATGACTCCGGAACAGCTTCGTGCCTTGGCGGCACAGGCGTTGCAGTTGCAGTCCCAG
GTCGAGGCGATGAGCCGGAAGATCCAGAACGACGAAATCCTCATCGAACAATTCAAGTTCGAAATCGCCCTGCTCAAACGCCACAAGTTTGCCAAGCGCA
GCGAGCAAATCAGCCCGGCGCAAGGCAGCTTGCTGGATGACCTGCTCGATACAGATCTTGAAGCTATCGAGGCCGAGCTGAAACAGCTTCTTCCCGCTTC
GTCACAAGCCGAGCCACGGCAATCGCCGAAACGCGCCCCATTGCCGCCGCAGTTTCCACGCGCGGTGATTCGTCACGAACCCGAAAACACCCAGTGCGCC
TGTGGCTGCCAACTCCAACGCATCGGCGAAGACGTCAGCGAGAAGCTGGATTACACGCCGGGGGTGTTTACCGTCGAGCAACATGTGCGTGGCAAATGGG
CCTGCCGTCAGTGCGAAACCCTGATCCAGGCGCCGGTGCCAGCCCAGGTTATTGATAAAGGTATCCCGACCGCAGGCTTGTTGGCCCACGTGATGGTGGC
CAAGTTTGCCGATCACTTACCGCTGTACCGGCAGGAGAAAATCCTTGGCCGCGCCGGGCTGGCAATTGCTCGCTCGACCTTGGCCCAGTGGGTCGGACAA
ACCGGCGTGCGACTCCAGCCGCTGGTCGATGCTCTGCGTGAAGCTGTGCTGAAGCAGGGCGTGATTCACGCTGACGAAACGCCGGTGCAGATGCTTGCGC
CAGGCGAGAAGAAAACTCACCGGGCTTATGTTTGGGCCTATAGCACGACGCCGTTTTCGGCGCTTAATGCGGTGGTTTACGACTTCAGCCCAAGCCGTGC
TGGCGAACATGCGCGCAACTTCCTGGGCGACTGGAGCGGCAAGTTGGTCTGCGATGACTTCGCTGGCTACAAAGCCGGGTTTGAGCAAGGCATCACTGAA
ATCGGCTGTATGGCCCACGCCCGTCGCAAGTTCTTTGATCTGCACGTAGCGAACAAAAGTCAGCTGGCCGAACAAGCGCTGCACTCGATCAGCGGCCTGT
ACGAGGTTGAACGCCAGGCGCGGGAAATGAGTGATGAAGATCGTTGGCGAATACGGCAGGAACTGGCGGTACCGATCAGCAAAACACTGCATGAGTGGAT
GTTGGCCCAGCGTGACCTGGTGCCCAACGGATCAGCCACAGCCAAAGCCCTCGACTACAGCCTGAAACGCTGGGTAGCGCTGACGCGCTACCTGGACGAT
GGATCTGTGCCCATCGACAACAATCAGGTCGAGAACCAGATACGGCCATGGGCACTCGGGCGCTCGAACTGGTTGTTTGCCGGGTCGCTGCGCAGTGGTA
AACGAGCGGCTGCGATCATGAGTTTGATCCAGTCGGCGCGCATGAACGGGCATGATCCGTATGCCTATCTCAAAGATGTGCTGACGCGGTTGCCGACACA
GCGGGCCAGTGAGATCGGGCAACTGCTGCCGCATCAGTGGGTATCGGCCTGACTTACGCAAGGTGCGTTGGGCGGACGCTTAC
ATGCGCCAACGAAGCTCTTATCCGAAACCGTTCAAAGCCCAGGTCGTTCAGGAATGCCTGCAACCTGGCGCAACCGTGTCCAGTGTCGCCATCAGCCACG
GCATCAATGCCAACGTCATCCGCAAATGGCTGCCGCTTTATCGAGATCAGCCTGCAGCAGCTTCAATACCGGCATTCGTCCCACTAAAAACCGCGCCTAA
ACGGCCAGTCGAAGCGTCAGTGATCATCGAGTTGCCTATGACCGGGCAAGTGATCACGGTGAAATGGCCAACCTCAGATCCTGAGGGCTGCGCGCAATTT
ATCCGGGCTGTCGCTCAATGATCCGCATCGATGCGATCTGGCTGGCCACCGAGCCGATGGATATGCGCGCCGGCACCGAAACGGCATTGGCCCGGGTGAT
CGCGGTGTTCGGTGCGGCGAAGCCGCACTGCGCTTATCTGTTCGCCAATCGCCGGGCCAACCGGATGAAGGTGCTGGTGCACGACGGCGTGGGCGTCTGG
CTTGCAGCGCGACGACTGAATCAAGGCAAGTTTCACTGGCCCGGCATTCGCCATGGCAGCGAGGTCGAACTCGACAGCGAACAACTCCAGGCGTTGGTAC
TGGGTTTGCCATGGCTGCGCGTTGGTGCAGGCGGTGTGATCAGCATGCTGTAATCGCCGGCCATTTGACCGGTCTGTTTGTCGTCGTTTCGGGCCTGCTC
TGGCACAATCGGCGGCATGACTTTCTCGCCCAATCTCGACCAGATGACTCCGGAACAGCTTCGTGCCTTGGCGGCACAGGCGTTGCAGTTGCAGTCCCAG
GTCGAGGCGATGAGCCGGAAGATCCAGAACGACGAAATCCTCATCGAACAATTCAAGTTCGAAATCGCCCTGCTCAAACGCCACAAGTTTGCCAAGCGCA
GCGAGCAAATCAGCCCGGCGCAAGGCAGCTTGCTGGATGACCTGCTCGATACAGATCTTGAAGCTATCGAGGCCGAGCTGAAACAGCTTCTTCCCGCTTC
GTCACAAGCCGAGCCACGGCAATCGCCGAAACGCGCCCCATTGCCGCCGCAGTTTCCACGCGCGGTGATTCGTCACGAACCCGAAAACACCCAGTGCGCC
TGTGGCTGCCAACTCCAACGCATCGGCGAAGACGTCAGCGAGAAGCTGGATTACACGCCGGGGGTGTTTACCGTCGAGCAACATGTGCGTGGCAAATGGG
CCTGCCGTCAGTGCGAAACCCTGATCCAGGCGCCGGTGCCAGCCCAGGTTATTGATAAAGGTATCCCGACCGCAGGCTTGTTGGCCCACGTGATGGTGGC
CAAGTTTGCCGATCACTTACCGCTGTACCGGCAGGAGAAAATCCTTGGCCGCGCCGGGCTGGCAATTGCTCGCTCGACCTTGGCCCAGTGGGTCGGACAA
ACCGGCGTGCGACTCCAGCCGCTGGTCGATGCTCTGCGTGAAGCTGTGCTGAAGCAGGGCGTGATTCACGCTGACGAAACGCCGGTGCAGATGCTTGCGC
CAGGCGAGAAGAAAACTCACCGGGCTTATGTTTGGGCCTATAGCACGACGCCGTTTTCGGCGCTTAATGCGGTGGTTTACGACTTCAGCCCAAGCCGTGC
TGGCGAACATGCGCGCAACTTCCTGGGCGACTGGAGCGGCAAGTTGGTCTGCGATGACTTCGCTGGCTACAAAGCCGGGTTTGAGCAAGGCATCACTGAA
ATCGGCTGTATGGCCCACGCCCGTCGCAAGTTCTTTGATCTGCACGTAGCGAACAAAAGTCAGCTGGCCGAACAAGCGCTGCACTCGATCAGCGGCCTGT
ACGAGGTTGAACGCCAGGCGCGGGAAATGAGTGATGAAGATCGTTGGCGAATACGGCAGGAACTGGCGGTACCGATCAGCAAAACACTGCATGAGTGGAT
GTTGGCCCAGCGTGACCTGGTGCCCAACGGATCAGCCACAGCCAAAGCCCTCGACTACAGCCTGAAACGCTGGGTAGCGCTGACGCGCTACCTGGACGAT
GGATCTGTGCCCATCGACAACAATCAGGTCGAGAACCAGATACGGCCATGGGCACTCGGGCGCTCGAACTGGTTGTTTGCCGGGTCGCTGCGCAGTGGTA
AACGAGCGGCTGCGATCATGAGTTTGATCCAGTCGGCGCGCATGAACGGGCATGATCCGTATGCCTATCTCAAAGATGTGCTGACGCGGTTGCCGACACA
GCGGGCCAGTGAGATCGGGCAACTGCTGCCGCATCAGTGGGTATCGGCCTGACTTACGCAAGGTGCGTTGGGCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 321 bp | 106 aa | 101 | 421 | + | No |
AG : IS66 TnpA
ORF sequence :
MRQRSSYPKPFKAQVVQECLQPGATVSSVAISHGINANVIRKWLPLYRDQPAAASIPAFVPLKTAPKRPVEASVIIELPMTGQVITVKWPTSDPEGCAQF
IRAVAQ
IRAVAQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 418 | 753 | + | No |
AG : IS66 TnpB
ORF sequence :
MIRIDAIWLATEPMDMRAGTETALARVIAVFGAAKPHCAYLFANRRANRMKVLVHDGVGVWLAARRLNQGKFHWPGIRHGSEVELDSEQLQALVLGLPWL
RVGAGGVISML
RVGAGGVISML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1536 bp | 511 aa | 817 | 2352 | + | No |
Chemistry : DDE
ORF sequence :
MTFSPNLDQMTPEQLRALAAQALQLQSQVEAMSRKIQNDEILIEQFKFEIALLKRHKFAKRSEQISPAQGSLLDDLLDTDLEAIEAELKQLLPASSQAEP
RQSPKRAPLPPQFPRAVIRHEPENTQCACGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPAQVIDKGIPTAGLLAHVMVAKFADH
LPLYRQEKILGRAGLAIARSTLAQWVGQTGVRLQPLVDALREAVLKQGVIHADETPVQMLAPGEKKTHRAYVWAYSTTPFSALNAVVYDFSPSRAGEHAR
NFLGDWSGKLVCDDFAGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSISGLYEVERQAREMSDEDRWRIRQELAVPISKTLHEWMLAQRD
LVPNGSATAKALDYSLKRWVALTRYLDDGSVPIDNNQVENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYAYLKDVLTRLPTQRASEI
GQLLPHQWVSA
RQSPKRAPLPPQFPRAVIRHEPENTQCACGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPAQVIDKGIPTAGLLAHVMVAKFADH
LPLYRQEKILGRAGLAIARSTLAQWVGQTGVRLQPLVDALREAVLKQGVIHADETPVQMLAPGEKKTHRAYVWAYSTTPFSALNAVVYDFSPSRAGEHAR
NFLGDWSGKLVCDDFAGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSISGLYEVERQAREMSDEDRWRIRQELAVPISKTLHEWMLAQRD
LVPNGSATAKALDYSLKRWVALTRYLDDGSVPIDNNQVENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYAYLKDVLTRLPTQRASEI
GQLLPHQWVSA
Blast result :
Comments
There are 6 intact, highly related, but different copies of ISPpu14 in this strain, and one copy lacks a direct repeat. One insertion disrupts a cellular gene.
ISPpu14 is 65% aa (ORF1) similar to ISSfl3, 55% (ORF2) to IS866, and 51% (ORF3) to ISRm14.
ISPpu14 is 65% aa (ORF1) similar to ISSfl3, 55% (ORF2) to IS866, and 51% (ORF3) to ISRm14.
References
1] DeBoy., R. (2002) Direct submission