ISPpu11
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE016775 | ND | Pseudomonas putida | Pseudomonas putida KT2440 |
DNA section
IS Length : 1348 bp
Ends
IR Length : 12
IRL : TAATCGATGGACTCGCCCCCGCCGAGGCATCACAGTGCCACGGTGGTGTT
IRR : ---TGTATGGACTCGCCCACACTGTCTAGCTGCTTTTTTGAACATAGGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATCGATGGACTCGCCCCCGCCGAGGCATCACAGTGCCACGGTGGTGTTCGAAGAAGCCAACGGCAGCGAGGGCGAGACCATGAAAAAGCATAGTTCGA
AACACGATTCCCTGTCTTCCACTGATGTGGAGCTTTGCACAACCATCTGCGTAGACCTGGCCAAACAGGTCTTCCAATTAGCAGGCGAGGACGCTACTGG
TCGGGTGATCTACGAAGATCGCATCAAATCCCGCCAAGCGTTCCACGATTTCCTGCTCAGGCTGCCAACGACCGTGGCCGTCTTGATGGAGTGCGGTCCA
GGTGCGCAAGCCTGGGCCAGGCTGCTGCAGGCCAAAGGTAATCCGGTTCGCATCCTGCCGGCGCAACGTGTCGCCGAACATCGCAGCGGTGCAAAGAACG
ATCGTAACGACTGCTATGCCATTTTGCGTGCCGGTCGCGATACGAGCATCGCTTCGATACCGATCAAGAGCACCACAGCACTGGCTATTCAAGCGCTGCA
CCGTATCCGGCGCGGTAACATCAAGCGACATACCGCGCTGGGTAATCAGATCCGTGGTTTGCTACTTGAGCATGGCGTATCCATGCCCCAGGGCGATGCC
GCGATCAATAAGCATGTGCCTCAGGCGCTTGAGGATGCTTCCTTGCCTCTACCCGACCTGTTGCGCGAGTTGCTCGATGAGTTGCTGATGGACTGGTTGT
CTCTAGGTGAACGCATAACGGCGCTGAGCCGCCGGCTCGAAGCGACCGCCCAAGAGGATAAAGTCGCTCAGCGGCTGATCACCATTCGCGGCGTCGGCCC
TATCATTGCCACGGCGATCGTGGCGAAACAGACCGACCCCAGCCGCTTCGCCAGTGGCCGAATGTATTCCGCCTTCTTCGGTATCGTGCCCGATCAGCAC
AGCAGCGGAAACAAAATCAGATTGGGCAAGATGAGCAAGCGCGGTGACGGCTACATACGCAGCCTGATGATCCAGGGCGCGCATTCCGTCTTGAGCCAGC
TAAGGCCTGATTCCGATCAGCCAGATGATCGCCGCCTCTTGCGCTGGCTATCCCGCCTTGGGCGCAAGGAGGCGGCGATCAGGCTGGCTAATCGAAATCT
GCGGATTATCTGGGCCCTGTTACAGAGCGATCAGGTTTACCAACGCAAACCGAACGAAAAACCGGAGGCGGCTATGAGCCTCTGACCAACCGGGTAATGC
CACCGGGGCGCTAGGCGCTCCAACCCCTGCTAGTGAACATTGACCCTAGGTAAGACCGTCGCAGACCGATGCCTCCGCTCCTACAGGCCTGATAGTGGCC
TCAATGTAAACGGCACACTGCGAGCTCACCACGATGTTGGCCAGAAGCAAAAAGCTTCCTCGAATAGGCCTGATACATAGATGCAACCGGGTTCCTATGT
TCAAAAAAGCAGCTAGACAGTGTGGGCGAGTCCATACA
AACACGATTCCCTGTCTTCCACTGATGTGGAGCTTTGCACAACCATCTGCGTAGACCTGGCCAAACAGGTCTTCCAATTAGCAGGCGAGGACGCTACTGG
TCGGGTGATCTACGAAGATCGCATCAAATCCCGCCAAGCGTTCCACGATTTCCTGCTCAGGCTGCCAACGACCGTGGCCGTCTTGATGGAGTGCGGTCCA
GGTGCGCAAGCCTGGGCCAGGCTGCTGCAGGCCAAAGGTAATCCGGTTCGCATCCTGCCGGCGCAACGTGTCGCCGAACATCGCAGCGGTGCAAAGAACG
ATCGTAACGACTGCTATGCCATTTTGCGTGCCGGTCGCGATACGAGCATCGCTTCGATACCGATCAAGAGCACCACAGCACTGGCTATTCAAGCGCTGCA
CCGTATCCGGCGCGGTAACATCAAGCGACATACCGCGCTGGGTAATCAGATCCGTGGTTTGCTACTTGAGCATGGCGTATCCATGCCCCAGGGCGATGCC
GCGATCAATAAGCATGTGCCTCAGGCGCTTGAGGATGCTTCCTTGCCTCTACCCGACCTGTTGCGCGAGTTGCTCGATGAGTTGCTGATGGACTGGTTGT
CTCTAGGTGAACGCATAACGGCGCTGAGCCGCCGGCTCGAAGCGACCGCCCAAGAGGATAAAGTCGCTCAGCGGCTGATCACCATTCGCGGCGTCGGCCC
TATCATTGCCACGGCGATCGTGGCGAAACAGACCGACCCCAGCCGCTTCGCCAGTGGCCGAATGTATTCCGCCTTCTTCGGTATCGTGCCCGATCAGCAC
AGCAGCGGAAACAAAATCAGATTGGGCAAGATGAGCAAGCGCGGTGACGGCTACATACGCAGCCTGATGATCCAGGGCGCGCATTCCGTCTTGAGCCAGC
TAAGGCCTGATTCCGATCAGCCAGATGATCGCCGCCTCTTGCGCTGGCTATCCCGCCTTGGGCGCAAGGAGGCGGCGATCAGGCTGGCTAATCGAAATCT
GCGGATTATCTGGGCCCTGTTACAGAGCGATCAGGTTTACCAACGCAAACCGAACGAAAAACCGGAGGCGGCTATGAGCCTCTGACCAACCGGGTAATGC
CACCGGGGCGCTAGGCGCTCCAACCCCTGCTAGTGAACATTGACCCTAGGTAAGACCGTCGCAGACCGATGCCTCCGCTCCTACAGGCCTGATAGTGGCC
TCAATGTAAACGGCACACTGCGAGCTCACCACGATGTTGGCCAGAAGCAAAAAGCTTCCTCGAATAGGCCTGATACATAGATGCAACCGGGTTCCTATGT
TCAAAAAAGCAGCTAGACAGTGTGGGCGAGTCCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1059 bp | 352 aa | 127 | 1185 | + | No |
Chemistry : DEDD
ORF sequence :
MELCTTICVDLAKQVFQLAGEDATGRVIYEDRIKSRQAFHDFLLRLPTTVAVLMECGPGAQAWARLLQAKGNPVRILPAQRVAEHRSGAKNDRNDCYAIL
RAGRDTSIASIPIKSTTALAIQALHRIRRGNIKRHTALGNQIRGLLLEHGVSMPQGDAAINKHVPRALEDASLPLPDLLRELLDELLMDWLSLGERITAL
SRRLEATAQEDKVAQRLITIRGVGPIIATAIVAKQTDPSRFASGRMYSAFFGIVPDQHSSGNKIRLGKMSKRGDGYIRSLMIQGAHSVLSQLRPDSDQPD
DRRLLRWLSRLGRKEAAIRLANRNLRIIWALLQSDQVYQRKPNEKPEAAMSL
RAGRDTSIASIPIKSTTALAIQALHRIRRGNIKRHTALGNQIRGLLLEHGVSMPQGDAAINKHVPRALEDASLPLPDLLRELLDELLMDWLSLGERITAL
SRRLEATAQEDKVAQRLITIRGVGPIIATAIVAKQTDPSRFASGRMYSAFFGIVPDQHSSGNKIRLGKMSKRGDGYIRSLMIQGAHSVLSQLRPDSDQPD
DRRLLRWLSRLGRKEAAIRLANRNLRIIWALLQSDQVYQRKPNEKPEAAMSL
Blast result :
Comments
There are 2 copies of ISPpu11 in this strain. ISPpu11 is 53% aa similar to IS1111C.
The IR of this IS are not at its termini. In the IS sequence as given 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 5 nt on the left and 4 on the right.
There are two copies of ISPpu11 in Pseudomonas putida KT2440. In the copy in AE016775, 25 bp including IRr are duplicated. In the copy in AE016787, ISPpu14 is inserted between the end of the ISPpu11 transposase and IRr.
The transposase protein is 36% identical to that of IS1111.
By analogy with IS4321, IS1618 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
The IR of this IS are not at its termini. In the IS sequence as given 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 5 nt on the left and 4 on the right.
There are two copies of ISPpu11 in Pseudomonas putida KT2440. In the copy in AE016775, 25 bp including IRr are duplicated. In the copy in AE016787, ISPpu14 is inserted between the end of the ISPpu11 transposase and IRr.
The transposase protein is 36% identical to that of IS1111.
By analogy with IS4321, IS1618 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] DeBoy., R. (2002) Direct submission
2] Nelson et al. (2002) Environ. Microbiol. 4, 799-808
3] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Nelson et al. (2002) Environ. Microbiol. 4, 799-808
3] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384