ISPpu8

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Pseudomonas putida
Pseudomonas putida KT2440
DNA section
IS Length : 1419 bp

Ends


IR Length : 18

IRL : CATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGA
IRR : CATCCCTTTCAAGGTCTTTTGGTTTTGGCGGCTTTGATGACCCGATCCGT

Insertion site


Left flankDirect repeatRight flankDR Length
TCACCTTTCCGCCCTTACGGCGGGTCCCTTT11
TCACCTTTTCGCCCTTACGGCGAGTCACTTT11
AAAGTGACCCGCCGTAAGGGCGGAAAGGTGA11
GGGGAATTTCGCCTTCAGGGTGGGTTTGCCT11
AAAGTGACTCGCCGTAAGGGCGAAAAGGTGA11

DNA sequence

CATCCCTTTCAAGGTCTTCGGTAGAATTCGCTGAAATCCAGTCAGGAAGACCCGAGCGATGTGGGCTGATGTACTAGCGCGATTCGAGAAAAAAGCACCC
GCCAGCGTAATGGCCAAACTTGCCTTGGAGCAGGCCATTGCGCCGGAGTGGGTCGATCAGGTTTTCGAGGAGCACCGCCAACGGCAGTATTCTCGCGAGC
TGCTGTTCTCGACCATTATCAAGTTGATGTCGCTTGTTTCATTGGGCTTGAAGCCATCGCTGCATGCTGCGGCAAGACAACTGGACGACCTTCCCGTCAG
CCTGGCAGCTCTGTACGACAAGATCAGTCGAACCGAACCTGCCCTGTTGCGTGCTCTGGTGACAGGCTGCGCGCAGCGCTTGGCGCCGACAATCCATGAG
TTGGGCTGCTCAGCCATGCTTCCTGATTGGCAAGTTCGGGTGGTCGATGGCAGCCACTTGGCCTCTACCGAAAAGCGTCTTGGCGCGTTGCGCCAAGAGC
GCGGAGCGGCTCGCCCCGGGTTTTCGGTGGTGGTTTACGACCCCGACCTGGATCAGGTAATTGACCTCCAGCCGTGCGAGGACGCCTACGCAAGCGAACG
AGTTTGTGTTTTGCCGTTGCTGGCTGAAGCAAAGACCAATCAGGTTTGGATCGCTGACCGTCTCTACTGCACGCTGCCTGTAATGGAGGCGTGCGAACAG
GTGAAGACATCATTTGTCATTCGCCAGCAGGCCAAGCATCCACGCTTGATCCAGGAAGGTGAGTGGCAAGCACCGATGCCCGTCGCCACAGGCACTGTGC
GCGAGCAATCCATCGAAGTAAAAGGCGGGCACCGCTGGCGACGTGTCGAGTTAACGCTTCATTCACCAAACGACTCAGGTGATAACAGCTTGATGTTCTG
GAGCAACCTGCCCGAGAGCATCAGTGCGCAACAGATCGCAGACTTCTATCGGCGCCGCTGGAGCATTGAAGGGATGTTCCAGCGGTTGGAAGCAATTCTG
GAAAGTGAAATTGAAACCCTCGGCAGTCCGCGAGCCGCCTTGCTTGGATTCACCACTGCCGTGCTGGCATACAACGTTCTGGCCTTGCTCAAGCGAAGTG
TTGAACAGGCTCACCGCGATGCCTTGCCCGAGAATTGGGAAGCCTCGATCTATCACTTGGCGGTGCAGATCAGAGGGGGTTACGAAGGCATGCAGATTGC
CCTGCCATCCGAGTACATGCCCGTTGTTCCGATGGAAAACCTGGCTCAGCGCCTGCTGGAGCTGGCCAGAAACATCCAGCCCAGACAGGTTGCGAAAAGC
CCACGAGGCCCCAAGGTGCTCAAAGCGAAGGCCTGGGTGCAAGGGACGGCTGTACATGCTCATGTTTCGACGGATCGGGTCATCAAAGCCGCCAAAACCA
AAAGACCTTGAAAGGGATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1302 bp433 aa1101411+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKLALEQAIAPEWVDQVFEEHRQRQYSRELLFSTIIKLMSLVSLGLKPSLHAAARQLDDLPVSLAALYDKISRTEPALLRALVTGCAQRLAPTIHELGC
SAMLPDWQVRVVDGSHLASTEKRLGALRQERGAARPGFSVVVYDPDLDQVIDLQPCEDAYASERVCVLPLLAEAKTNQVWIADRLYCTLPVMEACEQVKT
SFVIRQQAKHPRLIQEGEWQAPMPVATGTVREQSIEVKGGHRWRRVELTLHSPNDSGDNSLMFWSNLPESISAQQIADFYRRRWSIEGMFQRLEAILESE
IETLGSPRAALLGFTTAVLAYNVLALLKRSVEQAHRDALPENWEASIYHLAVQIRGGYEGMQIALPSEYMPVVPMENLAQRLLELARNIQPRQVAKSPRG
PKVLKAKAWVQGTAVHAHVSTDRVIKAAKTKRP

 

Blast result :
Comments
There are 6 copies of ISPpu8 in this strain, and one copy lacks a target site duplication. ISPpu8 is closely related (55% identity) to ISRSo13 in Ralstonia solanacearum GMI1000. One copy of ISPpu8 disrupts a cellular gene.
References
1] DeBoy., R. (2002) Direct submission