IS1664
- Family IS3
- Group IS150
Isoform Synonym(s) ISYe
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM286415 | ND | Yersinia enterocolitica | Yersinia enterocolitica subsp. enterocolitica 8081 Yersinia enterocolitica 8081c |
DNA section
IS Length : 1436 bp
Ends
IR Length : 21/26
IRL : TGAAGTGAACCCCGAAAGTTGGACACCCAGCGAGTAGGGGTTTTGCGTTT
IRR : TGAAGTGAACCCCATTTATTGGACACTTTTCCTGGCGGTTGACATGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAACCTGGG | ACTA | TGCTGGGTTT | 4 |
DNA sequence
TGAAGTGAACCCCGAAAGTTGGACACCCAGCGAGTAGGGGTTTTGCGTTTAATGGGTAAAAGAAAATACACAACTGAATTCAAGCGTCAGGTTGTTCTCC
ACTTCCTCAACAGTGATGATGGAGCCAGGCGTACCGCCCGACTATTTGGTCTTGACCACGGTACGGTTCGCCTCTGGACCGAGCACTGGAAAGCCAGCGG
AGAGAATGGCTTCACCATTACGACCAAAAAATATTCCGCTGCCTTCAAGGGGTCGGTACTTCTCTGGATGCAGGATAATCATCAGTCCTCCCGCAAAGCG
GCCGCAAAATTCAACATTGCGACAGCCTGCACCATCAACAGATGGGCGCGACTTTACCGCGAAGGGGGTATTATTGCGCTTCGCAACAAACCCAGAGGAC
GACCGACTATGTCAGGGAAGAAAAAACATTCTGCTCAGGAAGCAACGGCCCCTTACCCGGAGTTCCGTAGCATAGAAGAAGAGCTGGAATATCTGCGTGC
AGAGAACGCCTACTTAAAAAAGCTGCGTGCCTTGATTCAGGAGAAGCAGCGGAAAAAACGCGAATAATCACTGAATTAAGGCATCTGTATAAACTGGCGA
CCTTGCTTTACGTGGCCGGTCTTCCGCGCAGCACCTTCTACTGGCAGGTAAAATCCAGTGGTCGTGAAGAGACTTATGCGGATGAGAAACAAAGAATAAA
AACCCTGTTTCACCATCATAAAGGTCGCTATGGTTACCGCAGAATAACGCTGGCCCTACGCAATGAGGGCGGGAGTCTTAACCATAAAACCGTACGAAAA
CTCATGCGGCAACAGCAGCTTGCATCTAACTTGCGTCGTAAAAAATATCAGTCATATCAGGGTGCCTACGGTAAAGTGGTCCCTAATATACTTGCTCGAA
AATTTACTGCCGAAGCCCCTAACCAGAAGTGGGTAACGGATGTGACCGAGTTCAACGTCAGGGGTAAAAAGCTGTATCTGTCACCCGTGCTGGATCTGTA
TAACAGCGAAGTCGTTGCCTGGCAGATGGATACGCATCCCGGAATGAACCTGATTGATAAAATGCTTGATGATGCGCTACAGAAGTTGAACTCAGGCGAT
GAACCTGTACTTCACTCGGATCAGGGCTGGCAATATCAGATGGCAAGCTATCAGAAAAGGCTGGGGTCTGGCGAAGTAAAGCAAAGTATGTCGCGCAAGG
GCAACTGCCTGGATAATGCCGTGATAGAAAACTTTTTTGGTCTGCTGAAGACTGAATGCTGGCACAATGAAAAATACGAAGATGTAGAACAACTGAAAAA
AGCGGTGGATGAGTATATCCATTACTACAACAACGAAAGGATCAAAGTAAAATTAAACGGCCTGAGTCCGGTGCAGTACCGAAATCAGGCCATGTCAACC
GCCAGGAAAAGTGTCCAATAAATGGGGTTCACTTCA
ACTTCCTCAACAGTGATGATGGAGCCAGGCGTACCGCCCGACTATTTGGTCTTGACCACGGTACGGTTCGCCTCTGGACCGAGCACTGGAAAGCCAGCGG
AGAGAATGGCTTCACCATTACGACCAAAAAATATTCCGCTGCCTTCAAGGGGTCGGTACTTCTCTGGATGCAGGATAATCATCAGTCCTCCCGCAAAGCG
GCCGCAAAATTCAACATTGCGACAGCCTGCACCATCAACAGATGGGCGCGACTTTACCGCGAAGGGGGTATTATTGCGCTTCGCAACAAACCCAGAGGAC
GACCGACTATGTCAGGGAAGAAAAAACATTCTGCTCAGGAAGCAACGGCCCCTTACCCGGAGTTCCGTAGCATAGAAGAAGAGCTGGAATATCTGCGTGC
AGAGAACGCCTACTTAAAAAAGCTGCGTGCCTTGATTCAGGAGAAGCAGCGGAAAAAACGCGAATAATCACTGAATTAAGGCATCTGTATAAACTGGCGA
CCTTGCTTTACGTGGCCGGTCTTCCGCGCAGCACCTTCTACTGGCAGGTAAAATCCAGTGGTCGTGAAGAGACTTATGCGGATGAGAAACAAAGAATAAA
AACCCTGTTTCACCATCATAAAGGTCGCTATGGTTACCGCAGAATAACGCTGGCCCTACGCAATGAGGGCGGGAGTCTTAACCATAAAACCGTACGAAAA
CTCATGCGGCAACAGCAGCTTGCATCTAACTTGCGTCGTAAAAAATATCAGTCATATCAGGGTGCCTACGGTAAAGTGGTCCCTAATATACTTGCTCGAA
AATTTACTGCCGAAGCCCCTAACCAGAAGTGGGTAACGGATGTGACCGAGTTCAACGTCAGGGGTAAAAAGCTGTATCTGTCACCCGTGCTGGATCTGTA
TAACAGCGAAGTCGTTGCCTGGCAGATGGATACGCATCCCGGAATGAACCTGATTGATAAAATGCTTGATGATGCGCTACAGAAGTTGAACTCAGGCGAT
GAACCTGTACTTCACTCGGATCAGGGCTGGCAATATCAGATGGCAAGCTATCAGAAAAGGCTGGGGTCTGGCGAAGTAAAGCAAAGTATGTCGCGCAAGG
GCAACTGCCTGGATAATGCCGTGATAGAAAACTTTTTTGGTCTGCTGAAGACTGAATGCTGGCACAATGAAAAATACGAAGATGTAGAACAACTGAAAAA
AGCGGTGGATGAGTATATCCATTACTACAACAACGAAAGGATCAAAGTAAAATTAAACGGCCTGAGTCCGGTGCAGTACCGAAATCAGGCCATGTCAACC
GCCAGGAAAAGTGTCCAATAAATGGGGTTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 516 bp | 171 aa | 52 | 567 | + | No |
Description : First part of the transposase
ORF sequence :
MGKRKYTTEFKRQVVLHFLNSDDGARRTARLFGLDHGTVRLWTEHWKASGENGFTITTKKYSAAFKGSVLLWMQDNHQSSRKAAAKFNIATACTINRWAR
LYREGGIIALRNKPRGRPTMSGKKKHSAQEATAPYPEFRSIEEELEYLRAENAYLKKLRALIQEKQRKKRE
LYREGGIIALRNKPRGRPTMSGKKKHSAQEATAPYPEFRSIEEELEYLRAENAYLKKLRALIQEKQRKKRE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 471 | 1421 | + | No |
Description : Second part of the transposase
ORF sequence :
HRRRAGISACRERLLKKAACLDSGEAAEKTRIITELRHLYKLATLLYVAGLPRSTFYWQVKSSGREETYADEKQRIKTLFHHHKGRYGYRRITLALRNEG
GSLNHKTVRKLMRQQQLASNLRRKKYQSYQGAYGKVVPNILARKFTAEAPNQKWVTDVTEFNVRGKKLYLSPVLDLYNSEVVAWQMDTHPGMNLIDKMLD
DALQKLNSGDEPVLHSDQGWQYQMASYQKRLGSGEVKQSMSRKGNCLDNAVIENFFGLLKTECWHNEKYEDVEQLKKAVDEYIHYYNNERIKVKLNGLSP
VQYRNQAMSTARKSVQ
GSLNHKTVRKLMRQQQLASNLRRKKYQSYQGAYGKVVPNILARKFTAEAPNQKWVTDVTEFNVRGKKLYLSPVLDLYNSEVVAWQMDTHPGMNLIDKMLD
DALQKLNSGDEPVLHSDQGWQYQMASYQKRLGSGEVKQSMSRKGNCLDNAVIENFFGLLKTECWHNEKYEDVEQLKKAVDEYIHYYNNERIKVKLNGLSP
VQYRNQAMSTARKSVQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1370 bp | 456 aa | 52 | 1421 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKRKYTTEFKRQVVLHFLNSDDGARRTARLFGLDHGTVRLWTEHWKASGENGFTITTKKYSAAFKGSVLLWMQDNHQSSRKAAAKFNIATACTINRWAR
LYREGGIIALRNKPRGRPTMSGKKKHSAQEATAPYPEFRSIEEELEYLRAENAYLKKAACLDSGEAAEKTRIITELRHLYKLATLLYVAGLPRSTFYWQV
KSSGREETYADEKQRIKTLFHHHKGRYGYRRITLALRNEGGSLNHKTVRKLMRQQQLASNLRRKKYQSYQGAYGKVVPNILARKFTAEAPNQKWVTDVTE
FNVRGKKLYLSPVLDLYNSEVVAWQMDTHPGMNLIDKMLDDALQKLNSGDEPVLHSDQGWQYQMASYQKRLGSGEVKQSMSRKGNCLDNAVIENFFGLLK
TECWHNEKYEDVEQLKKAVDEYIHYYNNERIKVKLNGLSPVQYRNQAMSTARKSVQ
LYREGGIIALRNKPRGRPTMSGKKKHSAQEATAPYPEFRSIEEELEYLRAENAYLKKAACLDSGEAAEKTRIITELRHLYKLATLLYVAGLPRSTFYWQV
KSSGREETYADEKQRIKTLFHHHKGRYGYRRITLALRNEGGSLNHKTVRKLMRQQQLASNLRRKKYQSYQGAYGKVVPNILARKFTAEAPNQKWVTDVTE
FNVRGKKLYLSPVLDLYNSEVVAWQMDTHPGMNLIDKMLDDALQKLNSGDEPVLHSDQGWQYQMASYQKRLGSGEVKQSMSRKGNCLDNAVIENFFGLLK
TECWHNEKYEDVEQLKKAVDEYIHYYNNERIKVKLNGLSPVQYRNQAMSTARKSVQ
Blast result :
Comments
IS1664 is 69% aa similar to ISEcB1. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
August 1 2012 : the file of ISYe (Accession number : M29945 [P]) will be replaced by the most closely related sequence IS1664. Comments of the ISYe file :
ISYe is a vestigial element located 29 bp downstream of the ail (attachment invasion locus) gene of Yersinia enterocolitica (Miller et al., 1990). It is missing about 550 bp of its left end. ISYe DNA sequence shares 60 % DNA identity with IS150 (from coordinates 560 to 1443).
August 1 2012 : the file of ISYe (Accession number : M29945 [P]) will be replaced by the most closely related sequence IS1664. Comments of the ISYe file :
ISYe is a vestigial element located 29 bp downstream of the ail (attachment invasion locus) gene of Yersinia enterocolitica (Miller et al., 1990). It is missing about 550 bp of its left end. ISYe DNA sequence shares 60 % DNA identity with IS150 (from coordinates 560 to 1443).
References
1] Miller, V.L., Bliska, J.B., and Falkow, S. (1990) J. Bacteriol. 172, 1062-1069.
2] Delihas,N. (2003) BMC Microbiol. 3, 13.
3] Thomson,N.R., Howard,S., Wren,B.W., Holden,M.T., Crossman,L., Challis,G.L., Churcher,C., Mungall,K., Brooks,K., Chillingworth,T., Feltwell,T., Abdellah,Z., Hauser,H., Jagels,K., Maddison,M., Moule,S., Sanders,M., Whitehead,S., Quail,M.A., Dougan,G., Parkhill,J. and Prentice,M.B. (2006) PLoS Genet. 2 (12), E206 .
2] Delihas,N. (2003) BMC Microbiol. 3, 13.
3] Thomson,N.R., Howard,S., Wren,B.W., Holden,M.T., Crossman,L., Challis,G.L., Churcher,C., Mungall,K., Brooks,K., Chillingworth,T., Feltwell,T., Abdellah,Z., Hauser,H., Jagels,K., Maddison,M., Moule,S., Sanders,M., Whitehead,S., Quail,M.A., Dougan,G., Parkhill,J. and Prentice,M.B. (2006) PLoS Genet. 2 (12), E206 .