ISHsp2
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
JX569340 | Y | Halomonas sp. | Halomonas sp. ZM3 |
DNA section
IS Length : 1078 bp
Ends
IR Length : 16/26
IRL : GTGCCTGATCTTAAGTTTTCGTGTAACGTTGGAGTCTGATATCTCTAACG
IRR : GTGCTTATTCAAAATTAATCGCGTGATCAGCTTCATAGCTACCCAATAGC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TTCTAATTCT | TTAA | CTGGCCCATA | 4 |
DNA sequence
GTGCCTGATCTTAAGTTTTCGTGTAACGTTGGAGTCTGATATCTCTAACGGATGGAACCATGTTAAGGCGTTTCGTTGCTCCCCTTACTGACTCTGAACA
GCAGACGCTGTCCTCCGCCTATCACCACGGTGAGAAGCGTGCTCTACGACGCCGTGCACATGCCATCCTACTGAGCAATCAAGGCCATACGATCAACCAA
ATTAGCCAGATACTGGAGGTCCGCCGCGACGCCGTATCACGATGGCTCAAACAATGGGAAGCGTCAGGCCTCGATGGATTGATCGACAAGCCCCGCAGTG
GTCGCACGCCCGTCTTGGACGAGAGCGATCATCAGCGACTGCAGGAGCTCGTCGCCGAACAGCCTCACCAGATTCGATCCTTGCAGGCCCGTTTTGAAGA
AGAGACGGGCAAGACCGTCAGCACGGTGACCCTTCGCCGAGCGTTGAAAAAAAAATGACTTCAGCTTCAAACGTATCCGGCACTCACTCAAAGCATTGCG
TGACGAGACGGATTTCCGAAACACCCAAGGCCTCCTCAAGGCACTCCATCAGCAGGAGGACCAGGGTGAGCACGAGCTCTATTACTTTGATGAATCGGGT
TTTTCACAATCGTCGTCGGTACCCTATGCGTGGAGTCCCATCGGGCAACCCTGCGAGGTGACCGCCTACTCACACAGCCGAAGGCTGAACGTATTGGGGT
TCCTCAGCCGTGCTGGGAAACTGATCTACCACACCACTACCGGGTCAGTGACCACCGAGACGGTCATCGAAGCGTTTGACCAGTTTGTAGCACAGAAAAA
AACCGATACCTTCGCTGTTGTGGTGCTCGACAACGCCAGCATGCATCGCTCGCAGGCCTTCAGGCGGAAAATAGTCGAGTGGATGTCGCACCGTGTGCAT
CTGGTTTACTTATCGGCCTACTCGCCGGAGCTGAATCTGATTGAGATACTGTGGCGTCAGATGAAGTACGCATGGCTGCCGTTGAGTGCTTACCTTTCGT
TTGATCACCTCTGTGAAGAGGTACACCGGCTATTGGGTAGCTATGAAGCTGATCACGCGATTAATTTTGAATAAGCAC
GCAGACGCTGTCCTCCGCCTATCACCACGGTGAGAAGCGTGCTCTACGACGCCGTGCACATGCCATCCTACTGAGCAATCAAGGCCATACGATCAACCAA
ATTAGCCAGATACTGGAGGTCCGCCGCGACGCCGTATCACGATGGCTCAAACAATGGGAAGCGTCAGGCCTCGATGGATTGATCGACAAGCCCCGCAGTG
GTCGCACGCCCGTCTTGGACGAGAGCGATCATCAGCGACTGCAGGAGCTCGTCGCCGAACAGCCTCACCAGATTCGATCCTTGCAGGCCCGTTTTGAAGA
AGAGACGGGCAAGACCGTCAGCACGGTGACCCTTCGCCGAGCGTTGAAAAAAAAATGACTTCAGCTTCAAACGTATCCGGCACTCACTCAAAGCATTGCG
TGACGAGACGGATTTCCGAAACACCCAAGGCCTCCTCAAGGCACTCCATCAGCAGGAGGACCAGGGTGAGCACGAGCTCTATTACTTTGATGAATCGGGT
TTTTCACAATCGTCGTCGGTACCCTATGCGTGGAGTCCCATCGGGCAACCCTGCGAGGTGACCGCCTACTCACACAGCCGAAGGCTGAACGTATTGGGGT
TCCTCAGCCGTGCTGGGAAACTGATCTACCACACCACTACCGGGTCAGTGACCACCGAGACGGTCATCGAAGCGTTTGACCAGTTTGTAGCACAGAAAAA
AACCGATACCTTCGCTGTTGTGGTGCTCGACAACGCCAGCATGCATCGCTCGCAGGCCTTCAGGCGGAAAATAGTCGAGTGGATGTCGCACCGTGTGCAT
CTGGTTTACTTATCGGCCTACTCGCCGGAGCTGAATCTGATTGAGATACTGTGGCGTCAGATGAAGTACGCATGGCTGCCGTTGAGTGCTTACCTTTCGT
TTGATCACCTCTGTGAAGAGGTACACCGGCTATTGGGTAGCTATGAAGCTGATCACGCGATTAATTTTGAATAAGCAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
399 bp | 132 aa | 60 | 458 | + | No |
Description : First part of the transposase
ORF sequence :
MLRRFVAPLTDSEQQTLSSAYHHGEKRALRRRAHAILLSNQGHTINQISQILEVRRDAVSRWLKQWEASGLDGLIDKPRSGRTPVLDESDHQRLQELVAE
QPHQIRSLQARFEEETGKTVSTVTLRRALKKK
QPHQIRSLQARFEEETGKTVSTVTLRRALKKK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
627 bp | 192 aa | 448 | 1074 | + | No |
Description : Second part of the transposase
ORF sequence :
KKNDFSFKRIRHSLKALRDETDFRNTQGLLKALHQQEDQGEHELYYFDESGFSQSSSVPYAWSPIGQPCEVTAYSHSRRLNVLGFLSRAGKLIYHTTTGS
VTTETVIEAFDQFVAQKKTDTFAVVVLDNASMHRSQAFRRKIVEWMSHRVHLVYLSAYSPELNLIEILWRQMKYAWLPLSAYLSFDHLCEEVHRLLGSYE
ADHAINFE
VTTETVIEAFDQFVAQKKTDTFAVVVLDNASMHRSQAFRRKIVEWMSHRVHLVYLSAYSPELNLIEILWRQMKYAWLPLSAYLSFDHLCEEVHRLLGSYE
ADHAINFE
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1015 bp | 337 aa | 60 | 1074 | + | Yes |
Chemistry : DDE
ORF sequence :
MLRRFVAPLTDSEQQTLSSAYHHGEKRALRRRAHAILLSNQGHTINQISQILEVRRDAVSRWLKQWEASGLDGLIDKPRSGRTPVLDESDHQRLQELVAE
QPHQIRSLQARFEEETGKTVSTVTLRRALKKNDFSFKRIRHSLKALRDETDFRNTQGLLKALHQQEDQGEHELYYFDESGFSQSSSVPYAWSPIGQPCEV
TAYSHSRRLNVLGFLSRAGKLIYHTTTGSVTTETVIEAFDQFVAQKKTDTFAVVVLDNASMHRSQAFRRKIVEWMSHRVHLVYLSAYSPELNLIEILWRQ
MKYAWLPLSAYLSFDHLCEEVHRLLGSYEADHAINFE
QPHQIRSLQARFEEETGKTVSTVTLRRALKKNDFSFKRIRHSLKALRDETDFRNTQGLLKALHQQEDQGEHELYYFDESGFSQSSSVPYAWSPIGQPCEV
TAYSHSRRLNVLGFLSRAGKLIYHTTTGSVTTETVIEAFDQFVAQKKTDTFAVVVLDNASMHRSQAFRRKIVEWMSHRVHLVYLSAYSPELNLIEILWRQ
MKYAWLPLSAYLSFDHLCEEVHRLLGSYEADHAINFE
Blast result :
Comments
ISHsp2 has been identified by its integration to the entrapment vector pMAT1 (sacB cassette).
ISHsp2 is 67% aa similar to ISVsa8.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISHsp2 is 67% aa similar to ISVsa8.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Dziewit, L., Pyzik, A., Matlakowska, R., Baj, J., Szuplewska, M., Bartosik, D. (2013) BMC Microbiol. 13, 59
2] Dziewit,L. and Bartosik,D. (2012) Direct submission GenBank.
2] Dziewit,L. and Bartosik,D. (2012) Direct submission GenBank.