ISTesp1

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB004563 ND Terrabacter sp.
Terrabacter sp. DBF63
DNA section
IS Length : 1353 bp

Ends


IR Length : 15

IRL : TGAACCGCACTGGGTTGGTTGGAGTCTCCATCGTTTGGAAACGAGGATGT
IRR : TGAACCGCACTGGGTCTGATGGAGGCTCGGGCTATTGGATTCCGACCAAG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCCCGGATCCGGCGATGAGGAC3

DNA sequence

TGAACCGCACTGGGTTGGTTGGAGTCTCCATCGTTTGGAAACGAGGATGTACTCATGCCGAAGGACACCACCCCGGGGAAGCCGACCACGCGTCGCTACA
GCCCCGACGAGAAGGCCGCTGCGGTGCGGATGGTCAGGACTCTGCGCGCAGAGCTAGGCACAGAGCACGGCACGGTCCAGCGCGTCGCCCAGCAGTTGGG
GTACGGCACCGAGTCGGTGCGGTCCTGGGTCCGTCAAGCTGATATCGACGAGGGCCACGTCGCGGGCATCAGTACCAGTGACGCGGCGAAGATGAAGGCT
CTGGAGCAGGAGAACCGTGAGCTCAAGCGGGCCAACGAAATCTTGAAGAGGGCCGCCAGTTTCTTCGGGGCGGAGCTCGACCGCCAGCACAAGAAGTAGC
CGCGTTCATCGACGCCAACCGCGACGACGTCGTCGCGGGTCGGCGGCTGGGGGTCGAGCTCATCTGCAGCGTGCTGCAGGTGGCCCCCAGCAGCTACTAC
CAGGTCAAGAGCCGGCAGCCATCGGCCCGTGCCCGACGTGACGCGGTGATGGGCCCGGTGGTGCGGCAGCTGTGGGAAGCCAACTACCGGGTCTACGGGG
CACGGAAGATCTGGAAGGCCGCGCGACGCTCTGGTCACGACATCGGCCGCGACCAGGTCGCCCGGCTGATGCGCGCCGAGGGTATCGAGGGCGTGCGGCG
CACCAAGCGGGTGCGCACCACCACGCCAGACCCGGGCATGCCGCGGCACCCGGACCTGGTGGGCCGCGACTTCACCGCCACCGCGCCGAACCAGTTGTGG
GTCACAGATCTGACCTTCGTGCCGACCTGGGCCGGCGTCGCCTACGTCTGCTTCATCATCGACGCCTACTCCCGCACGATCGTCGGGTGGCGAGTCGCCT
CGCACATGAGGACCACGATGGTCCTCGACGCCATCGAGATGGCCAGGTGGTCACGCGGCACCCAGCTCGACGGGCTGCGGTGTCACAGCGACGCGGGCAG
CCAATTCACGTCGATTCGCTACGGAGAACGACTGGCTGAGATCGGTGCGGTGCCCTCGATCGGCACCGTCGGGGATTCGTACGATAACGCCCTTGCCGAG
ACCGTGAACGGTTACTACAAGGCCGAGCTAATCCGCGGCCCCGCACGCCAGGGACGGCCCTGGAAGAGCATCGAGGATGTCGAGCTCGCCACCCTGTCCT
GGGTCCACTGGCACAACCACGACCGCCTCCACGGCTACCTGGGCGACGTCCCGCCAGCAGAGTTCGAGGAAGCGTTCTACGTTACGCAACGGTCCGACCA
CCCCTTGGTCGGAATCCAATAGCCCGAGCCTCCATCAGACCCAGTGCGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
345 bp114 aa55399+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPKDTTPGKPTTRRYSPDEKAAAVRMVRTLRAELGTEHGTVQRVAQQLGYGTESVRSWVRQADIDEGHVAGISTSDAAKMKALEQENRELKRANEILKRA
ASFFGAELDRQHKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1002 bp333 aa3211322+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AQAGQRNLEEGRQFLRGGARPPAQEVAAFIDANRDDVVAGRRLGVELICSVLQVAPSSYYQVKSRQPSARARRDAVMGPVVRQLWEANYRVYGARKIWKA
ARRSGHDIGRDQVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTM
VLDAIEMARWSRGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNH
DRLHGYLGDVPPAEFEEAFYVTQRSDHPLVGIQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1268 bp422 aa551322+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPKDTTPGKPTTRRYSPDEKAAAVRMVRTLRAELGTEHGTVQRVAQQLGYGTESVRSWVRQADIDEGHVAGISTSDAAKMKALEQENRELKRANEILKRA
ASFLRGGARPPAQEVAAFIDANRDDVVAGRRLGVELICSVLQVAPSSYYQVKSRQPSARARRDAVMGPVVRQLWEANYRVYGARKIWKAARRSGHDIGRD
QVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTMVLDAIEMARWS
RGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNHDRLHGYLGDVP
PAEFEEAFYVTQRSDHPLVGIQ

 

Blast result :
Comments
ISTesp1 is 67% (ORF1) and 66% (ORF2) aa similar to IS987.
At position 31-44, there is a palindromic sequence : TCGTTTGGAAACGA.
At position 43-47, there is a putative RBS for transposase gene.
There is a frameshift site.
References
1] Kasuga,K., Nojiri,H., Yamane,H., Kodama,T. and Omori,T. (1997) J. Ferment. Bioeng. 84, 387-399