ISTesp1
- Family IS3
- Group IS51
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AB004563 | ND | Terrabacter sp. | Terrabacter sp. DBF63 |
DNA section
IS Length : 1353 bp
Ends
IR Length : 15
IRL : TGAACCGCACTGGGTTGGTTGGAGTCTCCATCGTTTGGAAACGAGGATGT
IRR : TGAACCGCACTGGGTCTGATGGAGGCTCGGGCTATTGGATTCCGACCAAG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGCCCGGATC | CGG | CGATGAGGAC | 3 |
DNA sequence
TGAACCGCACTGGGTTGGTTGGAGTCTCCATCGTTTGGAAACGAGGATGTACTCATGCCGAAGGACACCACCCCGGGGAAGCCGACCACGCGTCGCTACA
GCCCCGACGAGAAGGCCGCTGCGGTGCGGATGGTCAGGACTCTGCGCGCAGAGCTAGGCACAGAGCACGGCACGGTCCAGCGCGTCGCCCAGCAGTTGGG
GTACGGCACCGAGTCGGTGCGGTCCTGGGTCCGTCAAGCTGATATCGACGAGGGCCACGTCGCGGGCATCAGTACCAGTGACGCGGCGAAGATGAAGGCT
CTGGAGCAGGAGAACCGTGAGCTCAAGCGGGCCAACGAAATCTTGAAGAGGGCCGCCAGTTTCTTCGGGGCGGAGCTCGACCGCCAGCACAAGAAGTAGC
CGCGTTCATCGACGCCAACCGCGACGACGTCGTCGCGGGTCGGCGGCTGGGGGTCGAGCTCATCTGCAGCGTGCTGCAGGTGGCCCCCAGCAGCTACTAC
CAGGTCAAGAGCCGGCAGCCATCGGCCCGTGCCCGACGTGACGCGGTGATGGGCCCGGTGGTGCGGCAGCTGTGGGAAGCCAACTACCGGGTCTACGGGG
CACGGAAGATCTGGAAGGCCGCGCGACGCTCTGGTCACGACATCGGCCGCGACCAGGTCGCCCGGCTGATGCGCGCCGAGGGTATCGAGGGCGTGCGGCG
CACCAAGCGGGTGCGCACCACCACGCCAGACCCGGGCATGCCGCGGCACCCGGACCTGGTGGGCCGCGACTTCACCGCCACCGCGCCGAACCAGTTGTGG
GTCACAGATCTGACCTTCGTGCCGACCTGGGCCGGCGTCGCCTACGTCTGCTTCATCATCGACGCCTACTCCCGCACGATCGTCGGGTGGCGAGTCGCCT
CGCACATGAGGACCACGATGGTCCTCGACGCCATCGAGATGGCCAGGTGGTCACGCGGCACCCAGCTCGACGGGCTGCGGTGTCACAGCGACGCGGGCAG
CCAATTCACGTCGATTCGCTACGGAGAACGACTGGCTGAGATCGGTGCGGTGCCCTCGATCGGCACCGTCGGGGATTCGTACGATAACGCCCTTGCCGAG
ACCGTGAACGGTTACTACAAGGCCGAGCTAATCCGCGGCCCCGCACGCCAGGGACGGCCCTGGAAGAGCATCGAGGATGTCGAGCTCGCCACCCTGTCCT
GGGTCCACTGGCACAACCACGACCGCCTCCACGGCTACCTGGGCGACGTCCCGCCAGCAGAGTTCGAGGAAGCGTTCTACGTTACGCAACGGTCCGACCA
CCCCTTGGTCGGAATCCAATAGCCCGAGCCTCCATCAGACCCAGTGCGGTTCA
GCCCCGACGAGAAGGCCGCTGCGGTGCGGATGGTCAGGACTCTGCGCGCAGAGCTAGGCACAGAGCACGGCACGGTCCAGCGCGTCGCCCAGCAGTTGGG
GTACGGCACCGAGTCGGTGCGGTCCTGGGTCCGTCAAGCTGATATCGACGAGGGCCACGTCGCGGGCATCAGTACCAGTGACGCGGCGAAGATGAAGGCT
CTGGAGCAGGAGAACCGTGAGCTCAAGCGGGCCAACGAAATCTTGAAGAGGGCCGCCAGTTTCTTCGGGGCGGAGCTCGACCGCCAGCACAAGAAGTAGC
CGCGTTCATCGACGCCAACCGCGACGACGTCGTCGCGGGTCGGCGGCTGGGGGTCGAGCTCATCTGCAGCGTGCTGCAGGTGGCCCCCAGCAGCTACTAC
CAGGTCAAGAGCCGGCAGCCATCGGCCCGTGCCCGACGTGACGCGGTGATGGGCCCGGTGGTGCGGCAGCTGTGGGAAGCCAACTACCGGGTCTACGGGG
CACGGAAGATCTGGAAGGCCGCGCGACGCTCTGGTCACGACATCGGCCGCGACCAGGTCGCCCGGCTGATGCGCGCCGAGGGTATCGAGGGCGTGCGGCG
CACCAAGCGGGTGCGCACCACCACGCCAGACCCGGGCATGCCGCGGCACCCGGACCTGGTGGGCCGCGACTTCACCGCCACCGCGCCGAACCAGTTGTGG
GTCACAGATCTGACCTTCGTGCCGACCTGGGCCGGCGTCGCCTACGTCTGCTTCATCATCGACGCCTACTCCCGCACGATCGTCGGGTGGCGAGTCGCCT
CGCACATGAGGACCACGATGGTCCTCGACGCCATCGAGATGGCCAGGTGGTCACGCGGCACCCAGCTCGACGGGCTGCGGTGTCACAGCGACGCGGGCAG
CCAATTCACGTCGATTCGCTACGGAGAACGACTGGCTGAGATCGGTGCGGTGCCCTCGATCGGCACCGTCGGGGATTCGTACGATAACGCCCTTGCCGAG
ACCGTGAACGGTTACTACAAGGCCGAGCTAATCCGCGGCCCCGCACGCCAGGGACGGCCCTGGAAGAGCATCGAGGATGTCGAGCTCGCCACCCTGTCCT
GGGTCCACTGGCACAACCACGACCGCCTCCACGGCTACCTGGGCGACGTCCCGCCAGCAGAGTTCGAGGAAGCGTTCTACGTTACGCAACGGTCCGACCA
CCCCTTGGTCGGAATCCAATAGCCCGAGCCTCCATCAGACCCAGTGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
345 bp | 114 aa | 55 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
MPKDTTPGKPTTRRYSPDEKAAAVRMVRTLRAELGTEHGTVQRVAQQLGYGTESVRSWVRQADIDEGHVAGISTSDAAKMKALEQENRELKRANEILKRA
ASFFGAELDRQHKK
ASFFGAELDRQHKK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1002 bp | 333 aa | 321 | 1322 | + | No |
Description : Second part of the transposase
ORF sequence :
AQAGQRNLEEGRQFLRGGARPPAQEVAAFIDANRDDVVAGRRLGVELICSVLQVAPSSYYQVKSRQPSARARRDAVMGPVVRQLWEANYRVYGARKIWKA
ARRSGHDIGRDQVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTM
VLDAIEMARWSRGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNH
DRLHGYLGDVPPAEFEEAFYVTQRSDHPLVGIQ
ARRSGHDIGRDQVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTM
VLDAIEMARWSRGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNH
DRLHGYLGDVPPAEFEEAFYVTQRSDHPLVGIQ
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1268 bp | 422 aa | 55 | 1322 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKDTTPGKPTTRRYSPDEKAAAVRMVRTLRAELGTEHGTVQRVAQQLGYGTESVRSWVRQADIDEGHVAGISTSDAAKMKALEQENRELKRANEILKRA
ASFLRGGARPPAQEVAAFIDANRDDVVAGRRLGVELICSVLQVAPSSYYQVKSRQPSARARRDAVMGPVVRQLWEANYRVYGARKIWKAARRSGHDIGRD
QVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTMVLDAIEMARWS
RGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNHDRLHGYLGDVP
PAEFEEAFYVTQRSDHPLVGIQ
ASFLRGGARPPAQEVAAFIDANRDDVVAGRRLGVELICSVLQVAPSSYYQVKSRQPSARARRDAVMGPVVRQLWEANYRVYGARKIWKAARRSGHDIGRD
QVARLMRAEGIEGVRRTKRVRTTTPDPGMPRHPDLVGRDFTATAPNQLWVTDLTFVPTWAGVAYVCFIIDAYSRTIVGWRVASHMRTTMVLDAIEMARWS
RGTQLDGLRCHSDAGSQFTSIRYGERLAEIGAVPSIGTVGDSYDNALAETVNGYYKAELIRGPARQGRPWKSIEDVELATLSWVHWHNHDRLHGYLGDVP
PAEFEEAFYVTQRSDHPLVGIQ
Blast result :
Comments
ISTesp1 is 67% (ORF1) and 66% (ORF2) aa similar to IS987.
At position 31-44, there is a palindromic sequence : TCGTTTGGAAACGA.
At position 43-47, there is a putative RBS for transposase gene.
There is a frameshift site.
At position 31-44, there is a palindromic sequence : TCGTTTGGAAACGA.
At position 43-47, there is a putative RBS for transposase gene.
There is a frameshift site.
References
1] Kasuga,K., Nojiri,H., Yamane,H., Kodama,T. and Omori,T. (1997) J. Ferment. Bioeng. 84, 387-399