ISTcSa
- Family IS630
- Group
Isoform Synonym(s) ISY100
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U38915 | ND | Synechocystis sp. | Synechocystis sp. PCC6803 |
DNA section
IS Length : 947 bp
Ends
IR Length : 19/24
IRL : TAGTCATTTCAATTAACGATGAGAGAATTTAATGTAAAATTATGGAGTGT
IRR : TAGTAGTTTCAAATAAAGCTGAGACGCTAAACGCCACAGTAAGAACGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATATTTGT | TA | TTCTCCTTTT | 2 |
| TTTCTCGTAT | TA | CCTCTTAATT | 2 |
| ATTTTCCACT | TA | CTTTGGGATC | 2 |
DNA sequence
TAGTCATTTCAATTAACGATGAGAGAATTTAATGTAAAATTATGGAGTGTACAAAATGAACAGGTTTAAACAATGGCTTACAGTTTAGATTTAAGGCAAA
GGGTAGTAGCTTATATAGAAGCTGGAGGAAAAATAACTGAGGCTTCCAAGATATATAAAATAGGAAAAGCCTCGATATACAGATGGTTAAATAGAGTAGA
TTTAAGCCCAACAAAAGTAGAGCGTCGCCATAGGAAATTAGACTGGGAAGCTCTAAAAAAAGACGTAGAAGAAAATCCCGATGCAAGATTAGTAGACAGA
GCCAAGAAATTTGGAGTGAGGCCGAGTGCCGTATATTACGCATTAAAGAAAATGAAAATAAACAGAAAAAAAAAAGAACTACGTTATCGAGAAAGAAACC
GGGAGGAACGAGTTAAGTACTATAGAATGTTAAGAGAACTAATTAAGCTCTATGGTAGTCAAGCTATAGTTTACATAGATGAATCTGGATTCGAAGCAAT
CCAGGCTTGTATTTATGCCTGGTCAAAAAAAGGAAAAAAAGTTTATGAGATTAGACAAGGAAAAAGGGGAGTCAGAGAAAATCTAGTAGCAGGGAGAAGA
AAAGGAAAAAAAGATTTGATTGCGCCGATGGTTTTTACCGGGAGTTTGAATGCAGAAGGCTTTGAAGGATGGTTAAAATTATATTTGCTACCCTCCCTCG
ACATTCCATCAATATTAATAATGGATAATGCTCCTATTCATCGTAAAACTGCCATTAAAGAATTGGCTAAAGAAGCAGGTCATGAAGTTCTTTTTTTGCC
GAAATATTCTCCTGATTTAAATGATATTGAGCATGACTTTAGTGCCTTGAAACGAGCTAGAATGTACGCTCCTATTGACACGTCTCTTGATGAAATTATC
CGTTCTTACTGTGGCGTTTAGCGTCTCAGCTTTATTTGAAACTACTA
GGGTAGTAGCTTATATAGAAGCTGGAGGAAAAATAACTGAGGCTTCCAAGATATATAAAATAGGAAAAGCCTCGATATACAGATGGTTAAATAGAGTAGA
TTTAAGCCCAACAAAAGTAGAGCGTCGCCATAGGAAATTAGACTGGGAAGCTCTAAAAAAAGACGTAGAAGAAAATCCCGATGCAAGATTAGTAGACAGA
GCCAAGAAATTTGGAGTGAGGCCGAGTGCCGTATATTACGCATTAAAGAAAATGAAAATAAACAGAAAAAAAAAAGAACTACGTTATCGAGAAAGAAACC
GGGAGGAACGAGTTAAGTACTATAGAATGTTAAGAGAACTAATTAAGCTCTATGGTAGTCAAGCTATAGTTTACATAGATGAATCTGGATTCGAAGCAAT
CCAGGCTTGTATTTATGCCTGGTCAAAAAAAGGAAAAAAAGTTTATGAGATTAGACAAGGAAAAAGGGGAGTCAGAGAAAATCTAGTAGCAGGGAGAAGA
AAAGGAAAAAAAGATTTGATTGCGCCGATGGTTTTTACCGGGAGTTTGAATGCAGAAGGCTTTGAAGGATGGTTAAAATTATATTTGCTACCCTCCCTCG
ACATTCCATCAATATTAATAATGGATAATGCTCCTATTCATCGTAAAACTGCCATTAAAGAATTGGCTAAAGAAGCAGGTCATGAAGTTCTTTTTTTGCC
GAAATATTCTCCTGATTTAAATGATATTGAGCATGACTTTAGTGCCTTGAAACGAGCTAGAATGTACGCTCCTATTGACACGTCTCTTGATGAAATTATC
CGTTCTTACTGTGGCGTTTAGCGTCTCAGCTTTATTTGAAACTACTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 849 bp | 282 aa | 73 | 921 | + | No |
Chemistry : DDE
ORF sequence :
MAYSLDLRQRVVAYIEAGGKITEASKIYKIGKASIYRWLNRVDLSPTKVERRHRKLDWEALKKDVEENPDARLVDRAKKFGVRPSAVYYALKKMKINRKK
KELRYRERNREERVKYYRMLRELIKLYGSQAIVYIDESGFEAIQACIYAWSKKGKKVYEIRQGKRGVRENLVAGRRKGKKDLIAPMVFTGSLNAEGFEGW
LKLYLLPSLDIPSILIMDNAPIHRKTAIKELAKEAGHEVLFLPKYSPDLNDIEHDFSALKRARMYAPIDTSLDEIIRSYCGV
KELRYRERNREERVKYYRMLRELIKLYGSQAIVYIDESGFEAIQACIYAWSKKGKKVYEIRQGKRGVRENLVAGRRKGKKDLIAPMVFTGSLNAEGFEGW
LKLYLLPSLDIPSILIMDNAPIHRKTAIKELAKEAGHEVLFLPKYSPDLNDIEHDFSALKRARMYAPIDTSLDEIIRSYCGV
Blast result :
Comments
ISTcSa is inserted into an IS5Sa copy, duplicating 2 bp. ISTcSa putative transposase is 28 % and 23 % identical to those of ISRm2011-2 (274-aa overlap) and Tc1 (248-aa overlap), respectively.
There are 20 copies of ISTcSa in Synechocystis genome.
There are 20 copies of ISTcSa in Synechocystis genome.
References
1] Cassier-Chauvat, C., Poncelet, M., Villoing, S., and Chauvat F. "The nature of three insertion sequences from the cyanobacterium Synechocystis PCC6803 supports the hypothesis of trans-phylla and trans-kingdom gene transfers" (1996) EMBL Data Library, Rel. 47.
2] Cassier-Chauvat, C., Poncelet, M., and Chauvat F. (1997) . Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria. Gene 195 (2), 257-266.
2] Cassier-Chauvat, C., Poncelet, M., and Chauvat F. (1997) . Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria. Gene 195 (2), 257-266.