IST3091
- Family IS30
- Group
Isoform Synonym(s) IS3091
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U32113 | ND | Thiobacillus ferrooxidans | Thiobacillus ferrooxidans tfi-91 plasmid pTFI91 |
DNA section
IS Length : 1064 bp
Ends
IR Length : 14/19
IRL : TCTAGAATGAAGTGCAACATCTCCGGTCGAAGGATGGCGGAGGTGAGTGA
IRR : TCAAATTTGAAGCGCAACACCACTGTTTAACTGCATGATTTGCTGTGCTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TCTAGAATGAAGTGCAACATCTCCGGTCGAAGGATGGCGGAGGTGAGTGAATGGGGACGAGATATCAGCAGTTGCAGTCGGAGCAGAGGAACCAGATTCA
GCGAGGGCTGAACGAGGGGTTGAGTATGCGGGCCGTGGCCAAGCAGATAGGGAGGAGTCCCAGCACGGTCAGCCGGGAGGTACGCCGGGGTTTGGTGGGA
GAAACCTACGATGCGATACAAGGCCGGGAGGAGGCGCAGAGGCGCCTTCGTAAGGGGGTTAGAAAGCTGGTGGGAGGCGCGCCCTTAACCAACGCGGTGA
CACACGCTATCCTGCAAAGGAAATGGTCACCAGAGCAGGTGGCCGGGAGGTTGCGGATGGACTATCCCGAGGACAAGCAGTGGCGTGTCTCCCATGAGAC
CATTTATCAGTTCATCTATGCCCACCCGGCCGGTGAGCTGCGTAAGGCGCTGATAGCGGCGCTACGCCAGGGACACGCAAAGCGCAAGCCGCGCACACGC
GGGAAGGACCGGCGCGGACAACTGCGGAACATGCGTTCCATCGGGGAGCGTCCCTTGGAGGCCCAAGACCGCGAGATACCCGGCCACTGGGAAGGAGACT
TCATCAAAGGGGCTTTCAACGGCAGCGCCATTGGTACTCTGGTGGAGCGCAGCAGCCGTTTCGTGCTTCTGGTCAGGATGGAAGGCACCGATGCCGACGC
GGCCCTGGAGGGGTTCACCAGGCGCATGCGCACCTTGCCCAAGTCCATCCTGCGGACCCTCACCTATGACCAGGGCAAGGAGATGGCACGGCACGAGGAG
CTGGAGCGCAAGGTGGGCATCCGTATCTACTTTGCCGACCCGCATAGTCCTTGGCAGCGCCCAACCAACGAGAACACCAATGGTCTCCTGCGCCAGTATT
TCCCCAAAGGGACGGATTTATCAGGATATTCACAACGCCGCTTGACGCAGGTGGCGGAAGAGCTCAACAATCGCCCAAGAAAATCTTTGGGATTCCGAAC
GCCAGCAGAAGTAATAGCACAGCAAATCATGCAGTTAAACAGTGGTGTTGCGCTTCAAATTTGA
GCGAGGGCTGAACGAGGGGTTGAGTATGCGGGCCGTGGCCAAGCAGATAGGGAGGAGTCCCAGCACGGTCAGCCGGGAGGTACGCCGGGGTTTGGTGGGA
GAAACCTACGATGCGATACAAGGCCGGGAGGAGGCGCAGAGGCGCCTTCGTAAGGGGGTTAGAAAGCTGGTGGGAGGCGCGCCCTTAACCAACGCGGTGA
CACACGCTATCCTGCAAAGGAAATGGTCACCAGAGCAGGTGGCCGGGAGGTTGCGGATGGACTATCCCGAGGACAAGCAGTGGCGTGTCTCCCATGAGAC
CATTTATCAGTTCATCTATGCCCACCCGGCCGGTGAGCTGCGTAAGGCGCTGATAGCGGCGCTACGCCAGGGACACGCAAAGCGCAAGCCGCGCACACGC
GGGAAGGACCGGCGCGGACAACTGCGGAACATGCGTTCCATCGGGGAGCGTCCCTTGGAGGCCCAAGACCGCGAGATACCCGGCCACTGGGAAGGAGACT
TCATCAAAGGGGCTTTCAACGGCAGCGCCATTGGTACTCTGGTGGAGCGCAGCAGCCGTTTCGTGCTTCTGGTCAGGATGGAAGGCACCGATGCCGACGC
GGCCCTGGAGGGGTTCACCAGGCGCATGCGCACCTTGCCCAAGTCCATCCTGCGGACCCTCACCTATGACCAGGGCAAGGAGATGGCACGGCACGAGGAG
CTGGAGCGCAAGGTGGGCATCCGTATCTACTTTGCCGACCCGCATAGTCCTTGGCAGCGCCCAACCAACGAGAACACCAATGGTCTCCTGCGCCAGTATT
TCCCCAAAGGGACGGATTTATCAGGATATTCACAACGCCGCTTGACGCAGGTGGCGGAAGAGCTCAACAATCGCCCAAGAAAATCTTTGGGATTCCGAAC
GCCAGCAGAAGTAATAGCACAGCAAATCATGCAGTTAAACAGTGGTGTTGCGCTTCAAATTTGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1014 bp | 337 aa | 57 | 1070 | + | No |
Chemistry : DDE
ORF sequence :
MGTRYQQLQSEQRNQIQRGLNEGLSMRAVAKQIGRSPSTVSREVRRGLVGETYDAIQGREEAQRRLRKGVRKLVGGAPLTNAVTHAILQRKWSPEQVAGR
LRMDYPEDKQWRVSHETIYQFIYAHPAGELRKALIAALRQGHAKRKPRTRGKDRRGQLRNMRSIGERPLEAQDREIPGHWEGDFIKGAFNGSAIGTLVER
SSRFVLLVRMEGTDADAALEGFTRRMRTLPKSILRTLTYDQGKEMARHEELERKVGIRIYFADPHSPWQRPTNENTNGLLRQYFPKGTDLSGYSQRRLTQ
VAEELNNRPRKSLGFRTPAEVIAQQIMQLNSGVALQI
LRMDYPEDKQWRVSHETIYQFIYAHPAGELRKALIAALRQGHAKRKPRTRGKDRRGQLRNMRSIGERPLEAQDREIPGHWEGDFIKGAFNGSAIGTLVER
SSRFVLLVRMEGTDADAALEGFTRRMRTLPKSILRTLTYDQGKEMARHEELERKVGIRIYFADPHSPWQRPTNENTNGLLRQYFPKGTDLSGYSQRRLTQ
VAEELNNRPRKSLGFRTPAEVIAQQIMQLNSGVALQI
Blast result :
Comments
IST3091 length is based on comparison with the other members of the IS30 family, more specifically with those containing the two CAA iterons in their left IR. Therefore, the right IR of IST3091 is presumably incomplete, and probably too short by 7 bp !
References
1] Chakravarty, L., Kittle, T., and Tuovinen, O. (1995) "Putative plasmid origin and Insertion Element of pTFI91 plasmid of Thiobacillus ferrooxidans". EMBL Data Library, Rel. 45.
2] Chakravarty, L., Kittle, T., and Tuovinen, O. (1997) Can. J. Microbiol. 43, 503-508.
2] Chakravarty, L., Kittle, T., and Tuovinen, O. (1997) Can. J. Microbiol. 43, 503-508.