ISSs1
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M93569 | ND | Synechococcus sp. | Synechococcus sp. PCC7002 |
DNA section
IS Length : 1481 bp
Ends
Left end : CTTAACAGCGGGGAAGCCCCGCGTTGTATGTGAAATATCAACGTCGGGATGAGGAGCGAGCGACTGACCAGCGGGAAGCAGCAAACTCCTAGTTAAGCGT II struct. : Yes
Right end : TGGAGCTACGCCCAGTAGGGAGGCTTTGAAGCAGGAATTTCTTAAAGCGATTTAAGAAGCCCGCGCTGTAATCTTTGATTCAGCGTCGGGAGTATGTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GATTTTTTTGC | TTAG | GGAAGACTCA | tcac |
DNA sequence
CTTAACAGCGGGGAAGCCCCGCGTTGTATGTGAAATATCAACGTCGGGATGAGGAGCGAGCGACTGACCAGCGGGAAGCAGCAAACTCCTAGTTAAGCGT
CTTAGAAAAAGTGTACGATTTCCCTAAGTCTTACTATGGTACAGGTATGATTAACCTGACTTACGAGTACAGAGTCTATCCGACTGCCGACCAAGCCATC
AAAATGGATGAATGGTTGGACACGTGCAAGCGCGTCTATAACTACGCCTTAGCTGTTGGACACGTGCAAGCGCGTCTATAACTACGCCTTAGCTGAACGT
CGTGATTGGATTCAGTCCCGTAAGTGCCCGATTAATGCTTGCAGTATTCAGCGGGAATTTATTTACCCAATGGATTCAGAAAAGCCGACCTATTACAGTC
AAAAGCGCAATTTGACCGCCGCAAGGAAAACTAATTCTTTTCTCAAGCGGTGCATTCTCAAGTGCTTCAGGATGTGATGGGCAGGCTGGAAAAGGCGTTT
AATGCATTGTGGAATAGTGGGTTCGGATTCCCACGGTTTAAGAAGCGTTTTCGCAGCTTTAGCTTCCCGCAGTTGGGCAAAAACCCAATTGGTGACAATC
AAATTAAGTTGCCTGTATTTGGTTGGGTTAAGACCGTGATGCACCGCCCGCTCCCTGATGGGTTTGAGGTGAAGCAAGCACGGGTAGTTAAACGCGCTTC
TGGTTGGTATATCCAGCTAGTTATCCAAAGCGACGTAAAGATTCCAGATCCCCCGATTGGTGGTTATGCCATTGGGATTGATGTGGGGTTAAGTCATTTT
GTTGCGACATCAGAAGGCGAGTTGATTGGGAGACCCCGATTCTTTGTGGATGCACAACACAGGCTGAAAGTGCTGAACCGTGCCGTTGCCAGAAAGCAGA
AAGGTTCAAAGAACCAGCAAAAAGCTCGTCAACGAGTCGCCAGATTCTATGAACGAATTGCGAACCGACGCAAAGATTTTCACCGTAAGTTAGCCCATCA
TCTCTGCGACCAAGCAGAGATGATATTTGCGGAGGAGTTGAACCTAAAAGGACTGGCAAAAGGAATGCTGGGGAAGCATTGTCTGGATGCTGGTTGGGGG
CAGTTCCTCGAGACATTGAGATGGGTCTGCTTTAAGCGTGGTGTGCATTTCCAAAAGGTTGCTGCTAATGGCACGAGTCAGGAATGCCCATCCTGTGGTG
TAGCGGTGAAAAAAGACTTGTCCATGCGTCGGCATGAATGCCCAGAATGTGGCTACACCACCAATCGAGATGTGGCCAGTGGTCAAGTAATTCGCAAAGC
ACCTGATCGATCGTGACTCGTACAACGACTCGAACAGCTAGCATGTCGAAGTGTAGCGGTTGGACAGACCGTTCAATCCTGTGGAGCTACGCCCAGTAGG
GAGGCTTTGAAGCAGGAATTTCTTAAAGCGATTTAAGAAGCCCGCGCTGTAATCTTTGATTCAGCGTCGGGAGTATGTCAC
CTTAGAAAAAGTGTACGATTTCCCTAAGTCTTACTATGGTACAGGTATGATTAACCTGACTTACGAGTACAGAGTCTATCCGACTGCCGACCAAGCCATC
AAAATGGATGAATGGTTGGACACGTGCAAGCGCGTCTATAACTACGCCTTAGCTGTTGGACACGTGCAAGCGCGTCTATAACTACGCCTTAGCTGAACGT
CGTGATTGGATTCAGTCCCGTAAGTGCCCGATTAATGCTTGCAGTATTCAGCGGGAATTTATTTACCCAATGGATTCAGAAAAGCCGACCTATTACAGTC
AAAAGCGCAATTTGACCGCCGCAAGGAAAACTAATTCTTTTCTCAAGCGGTGCATTCTCAAGTGCTTCAGGATGTGATGGGCAGGCTGGAAAAGGCGTTT
AATGCATTGTGGAATAGTGGGTTCGGATTCCCACGGTTTAAGAAGCGTTTTCGCAGCTTTAGCTTCCCGCAGTTGGGCAAAAACCCAATTGGTGACAATC
AAATTAAGTTGCCTGTATTTGGTTGGGTTAAGACCGTGATGCACCGCCCGCTCCCTGATGGGTTTGAGGTGAAGCAAGCACGGGTAGTTAAACGCGCTTC
TGGTTGGTATATCCAGCTAGTTATCCAAAGCGACGTAAAGATTCCAGATCCCCCGATTGGTGGTTATGCCATTGGGATTGATGTGGGGTTAAGTCATTTT
GTTGCGACATCAGAAGGCGAGTTGATTGGGAGACCCCGATTCTTTGTGGATGCACAACACAGGCTGAAAGTGCTGAACCGTGCCGTTGCCAGAAAGCAGA
AAGGTTCAAAGAACCAGCAAAAAGCTCGTCAACGAGTCGCCAGATTCTATGAACGAATTGCGAACCGACGCAAAGATTTTCACCGTAAGTTAGCCCATCA
TCTCTGCGACCAAGCAGAGATGATATTTGCGGAGGAGTTGAACCTAAAAGGACTGGCAAAAGGAATGCTGGGGAAGCATTGTCTGGATGCTGGTTGGGGG
CAGTTCCTCGAGACATTGAGATGGGTCTGCTTTAAGCGTGGTGTGCATTTCCAAAAGGTTGCTGCTAATGGCACGAGTCAGGAATGCCCATCCTGTGGTG
TAGCGGTGAAAAAAGACTTGTCCATGCGTCGGCATGAATGCCCAGAATGTGGCTACACCACCAATCGAGATGTGGCCAGTGGTCAAGTAATTCGCAAAGC
ACCTGATCGATCGTGACTCGTACAACGACTCGAACAGCTAGCATGTCGAAGTGTAGCGGTTGGACAGACCGTTCAATCCTGTGGAGCTACGCCCAGTAGG
GAGGCTTTGAAGCAGGAATTTCTTAAAGCGATTTAAGAAGCCCGCGCTGTAATCTTTGATTCAGCGTCGGGAGTATGTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1290 bp | 429 aa | 147 | 1436 | + | No |
AG : TnpB
ORF sequence :
MINLTYEYRVYPTADQAIKMDEWLDTCKRVYNYALAVGHVQARL*LRLS*TS*LDSVP*VPD*CLQYSAGIYLPNGFRKADLLQSKAQFDRRKEN*FFSQ
AVHSQVLQDVMGRLEKAFNALWNSGFGFPRFKKRFRSFSFPQLGKNPIGDNQIKLPVFGWVKTVMHRPLPDGFEVKQARVVKRASGWYIQLVIQSDVKIP
DPPIGGYAIGIDVGLSHFVATSEGELIGRPRFFVDAQHRLKVLNRAVARKQKGSKNQQKARQRVARFYERIANRRKDFHRKLAHHLCDQAEMIFAEELNL
KGLAKGMLGKHCLDAGWGQFLETLRWVCFKRGVHFQKVAANGTSQECPSCGVAVKKDLSMRRHECPECGYTTNRDVASGQVIRKAPDRS*LVQRLEQLAC
RSVAVGQTVQSCGATPSREALKQEFLKAI
AVHSQVLQDVMGRLEKAFNALWNSGFGFPRFKKRFRSFSFPQLGKNPIGDNQIKLPVFGWVKTVMHRPLPDGFEVKQARVVKRASGWYIQLVIQSDVKIP
DPPIGGYAIGIDVGLSHFVATSEGELIGRPRFFVDAQHRLKVLNRAVARKQKGSKNQQKARQRVARFYERIANRRKDFHRKLAHHLCDQAEMIFAEELNL
KGLAKGMLGKHCLDAGWGQFLETLRWVCFKRGVHFQKVAANGTSQECPSCGVAVKKDLSMRRHECPECGYTTNRDVASGQVIRKAPDRS*LVQRLEQLAC
RSVAVGQTVQSCGATPSREALKQEFLKAI
Blast result :
Comments
ISSs1 has been found by Donadio and Staver (1993) through its similarities to IS1136A.
This putative element is located downstream of the cpc (phycobilisome = major light-harvesting antennae for photosynthesis in cyanobacteria) operon.
File updated the July 17 2012.
The transposase shares a lot of STOP codons in frame due to mutations by insertion and deletions.
This putative element is located downstream of the cpc (phycobilisome = major light-harvesting antennae for photosynthesis in cyanobacteria) operon.
File updated the July 17 2012.
The transposase shares a lot of STOP codons in frame due to mutations by insertion and deletions.
References
1] Zhou, J., Gasparich, G.E., Stirewalt, V.L., de Lorimier, R., and Bryant, D.A. (1992) J. Biol. Chem. 267, 16138-16145.
2] Donadio, S., and Staver, M.J. (1993) Gene 126, 147-151.
2] Donadio, S., and Staver, M.J. (1993) Gene 126, 147-151.