ISSfl7

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Shigella flexneri
Shigella flexneri
DNA section
IS Length : 916 bp

Ends


IR Length : 16

IRL : GGCTGTGTCCCTTAATCATCTGAGTTATAGTAACTGTCTGTTTACACAAC
IRR : GGCTGTGTCCCTTAATTACGTAAGCGTTGATAAAAGAGTCGGATGCAGCC

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNACTAGTNNNNNNNN2

DNA sequence

GGCTGTGTCCCTTAATCATCTGAGTTATAGTAACTGTCTGTTTACACAACATATTGAACTATGGCTCGCTACGACCTTCCCGATGAGGCATGGACCATCA
TCAAGCCCCTGTTACCTCCTGAACCCGCCACACCACGGGCCGGACGCCCATGGGCTGAGCACCGTAAAATCATCAATGGCATGTTCTGGGTGTTATGTTC
CGGTGCACCATGGCGCGATTTACCCGAACGATATGGGGCATGGAAAACTGTTTATAACCGCTTTAACCGATGGTCAAAGTCAGGCGTGATTAATATTATT
TTCAACAGGTTGCTTTCGCTACTTGATGCAAACGGCTTTATTGACTGGTCTGCCACCGCGCTGGATGGCAGTAATATCCGGGCGCTGAAATGTGCCGCCG
GTACTCAAAAAAACATCCCGATATCGACGGAGATAATGGGCCGGGTCGCTCTCGCGGCGGTTTTGGCACCAAAATCCATCTGGCAACAGACGGAAGTGGC
CTCCCGTTAAATATCGTGCTAAGCCCCGGACAAGCTCATGAAAGCCAGTTCGCGCAACGACTTCTGGACGGGATTGGCGTTCAGCGTCAGAACGGCAGCA
TGAAACGCCGTGGACATGCGGTACTGGCTGATAAAGCGTATTCAGGGCGCGCATTGCGCAATGAGCTGAAAAATAACGGTATAAAGGCAGTAATCCCCCG
AAAGTCAAATGAGAAAATGGCATCGGATGGACGTGCACAGCTTGATCGTGATGCGTACTGCAATCGTAATGTCGTTGAACGGTGCTTTGGGCGGCTGAAA
GAATATCGCCGCATCGCCACGCGTTACGACAAAACGGCGAGAAATTACCTGGCGATAGTGAAACTGGGCTGCATCCGACTCTTTTATCAACGCTTACGTA
ATTAAGGGACACAGCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
450 bp149 aa61510+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWVLCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATA
LDGSNIRALKCAAGTQKNIPISTEIMGRVALAAVLAPKSIWQQTEVASR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
531 bp176 aa375905+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YPGAEMCRRYSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKN
NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
845 bp281 aa61905+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWVLCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATA
LDGSNIRALKCAAGTQKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALR
NELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN

 

Blast result :
Comments
ISSfl7 is 94% aa similar to ISEhe2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
File updated: July 10 2012.
References
1] Parsot. (1999) Direct submission.