ISfinder Mirror Site

ISDeal1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Dethiobacter alkaliphilus	Dethiobacter alkaliphilus AHT 1

DNA section

IS Length : 2525 bp

Ends

IR Length : 18/25

IRL : GTAAGCGTCCAATAAGTCGGTGTATGGCAACTCCCCTAATTCTATGAGAC
IRR : GTAAGCGCTTAATAAGATAGTGGATAGAAAAAAAGCAAAAACCAGTGTAA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCAAATTCTC	CATcCAAAC	ACCGTTAGCG	9
ATAACCCGTT	TTTAAAAC	AATTAAAACA	8

DNA sequence

GTAAGCGTCCAATAAGTCGGTGTATGGCAACTCCCCTAATTCTATGAGACAATTCCTTAAATTAGAGTTTTTAGAGTTTTTCGACCTGGTCGAAAAACTC
CAAAGGAGGTCTAGTATTGAACACTAGGGAAGTTGCTAAAAAATACCGGCTGAATCAATGGACTCAAATTATCCGGGAATGTCGTAGCAGCGGGCAAACA
GTCAAATCATGGTGTGCCGATAATGGTATCAATCAAAAAACTTACTACTATTGGTTGCGGAAAGTACGTGAGGCGGCCTGCGAAAGCCTCCCATCTCTCG
AGGAAAACAGCTCAATTGTACCCGTAAATATTCCGGCAAATGCAGCCGGGCCAGGTCCAGAGTCGTCTGCACAAATTATCCTGCGTTTTGGTTCAGTAAC
TTTGGAGCTCCGCAACAATGCCTCGGAGAAGTTGATCGAAAACACTCTAAGGGCACTTCAGAATGTTCGGTGATATCTCAAAGGCTGAAAAGGTCTACAT
TGCTTGTGGATATACCGACATGAGAAAAGCCATTGATGGGTTAGCTGCTATTGTCCAGCAGAACTTTCAGCTAAATCCCTTTCAGAATAGCGTGTTTCTC
TTCTGCGGCCGGCGCCGTGACCGACTAAAAGCGCTTTACTGGGAAGAAGATGGATTCGTGCTCCTGTACAAGCGATTAGAAAACGGGAAATTCCAATGGC
CGATGAGTGCCGAAGATGTTCGCACCATCACCAATCAGGAGTTCAGATGGCTCCTGGAGGGACTGTCCATTGACCAACCCAAGGCTGTTAAAAAGCTAAA
ATTAGAGTGTGGAATTTAGCTATAAAAAGTGCCGACAATGCCCATAAATACTGGCTTTTTGGAGGTTTTTATGTTATACTAAATATAGCAAAAACACCAA
AGGAAACCATCATTTATGAGCGCATCCGAGCAGTTAAAAAAGTTAGAGAACCGTATATCCGAACTGGAGCAGGAAAACAAAAAGCTCCATGATACAGTCG
ATCATTTGACACGTAAACTTTTCGGAAAAAGCTCTGAGAAGACATCGTCTCTACCTATGGGACAGATGTCTCTTTTTGATGAAGCAGAAATTACGGCTGA
TCCAAAGGCTCCAGAGCCTGATCTCAAGGAAGTACAGGGATATCGCAGAAAGAAGTTTCCCGGCCAACGGGCGGAATTACTAAAGGATCTTCCGCGGGAC
AAACGTCTCTGTACGCTAGTTGAAATGGATCGCTTCTGTGAAGAATGCGACACCACCCTTGTATCTGTCGGTGAAGAATTCGTCCGTACCGAAATTGAGT
TCATTCCTGCCAAAGTCAGGGTGATCGATTACTACCGTGAAACCTTTGAGTGTCGTACCTGTCGTAAGGATGGGAAACCCTACATGGAGAAATCTCCGAT
GCCCGATCCTGTGATCCAGCACTCCATGGCATCTCCATCCACAGTCGCCTGGGTCATGCATCAGAAATTCGTCAACGCCCTTCCCCTTTACCGGCAGGAA
AAGGAATGGCAGTCGTTAGGGGTTAGCTTAAGCCGTGCGACGATGGCCAACTGGATTATGGTTGCCTCCCGTGACTGGCTGTTGCCCTTGGTAGAGCTGA
TGCGCAAGAAGCTGTTGGATGAACATTACTTGCACGTTGATGAAACCACTGTGCAGGTAATGAATGAAAAGGGACGTAAGAATACAACCGATTCTTACAT
GTGGGTGTACAGCTCCGGCAAGCATGCCGAACACGCCATCCGGCTCTTCGAGTATCAGCCGGGAAGGAGTGGCAAATACCCGCAGGAGTTCCTCAAAGAC
TTCAAAGGTTATCTTCACTCCGATGCTTATGTCGGCTACAAGAAGATTCCTGAAGTTACAAGATGCCTCTGTTGGGCGCATGTCCGGCGGAAGTTCGTGG
ATGCGCTTCCGAAAGACATTCATCGCCCGGAAGCCACCCTTGCAAGCGAAGGCATTGCCTTCTGCAACAAGCTTTTTGAGATTGAAAAGGCTCTCGAAGA
GCTTTCAAGTGAAGATCGCAGATTAGAGCGTGTGAAGCAGGAAAAACCTGTTTTGGATGCTTTTTGGGCATGGATTCATTCCAACAAAGACAAGGTTCTC
CCAAAGTCCAAATTAAGCGAAGCCTTGCACTATGCTCTGAACAATAAGGAAGAGCTCATCAATTATCTTAAAGACGGCAACTGCTCCATTTCCAACAATC
TTGCAGAAAACAGTATCCGTCCCTTTACAATTGGACGAAAGAACTGGCTCTTTAGCGGAAGCCCAAAGGGTGCTACAGCTAGCGCAGCCGTTTACAGCAT
CATTGAAAGTGCCAAGGCCAATGGCCTGAACCCATACAAGTATCTGCATTTCATCTTTAGCCAACTTCCTGGCGTATTATTTAGGCAGCATCCAGAGTTT
CTGGAAGACTGTCTCCCTTGGAGCCAGGAGGTTCAGGAAAACTGCAAATAGAAGAAACGAGGGCAAAAACTTAGTTTACACTGGTTTTTGCTTTTTTTCT
ATCCACTATCTTATTAAGCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	117	473	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

LNTREVAKKYRLNQWTQIIRECRSSGQTVKSWCADNGINQKTYYYWLRKVREAACESLPSLEENSSIVPVNIPANAAGPGPESSAQIILRFGSVTLELRN
NASEKLIENTLRALQNVR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	463	819	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MFGDISKAEKVYIACGYTDMRKAIDGLAAIVQQNFQLNPFQNSVFLFCGRRRDRLKALYWEEDGFVLLYKRLENGKFQWPMSAEDVRTITNQEFRWLLEG
LSIDQPKAVKKLKLECGI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1536 bp	511 aa	916	2451	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSASEQLKKLENRISELEQENKKLHDTVDHLTRKLFGKSSEKTSSLPMGQMSLFDEAEITADPKAPEPDLKEVQGYRRKKFPGQRAELLKDLPRDKRLCT
LVEMDRFCEECDTTLVSVGEEFVRTEIEFIPAKVRVIDYYRETFECRTCRKDGKPYMEKSPMPDPVIQHSMASPSTVAWVMHQKFVNALPLYRQEKEWQS
LGVSLSRATMANWIMVASRDWLLPLVELMRKKLLDEHYLHVDETTVQVMNEKGRKNTTDSYMWVYSSGKHAEHAIRLFEYQPGRSGKYPQEFLKDFKGYL
HSDAYVGYKKIPEVTRCLCWAHVRRKFVDALPKDIHRPEATLASEGIAFCNKLFEIEKALEELSSEDRRLERVKQEKPVLDAFWAWIHSNKDKVLPKSKL
SEALHYALNNKEELINYLKDGNCSISNNLAENSIRPFTIGRKNWLFSGSPKGATASAAVYSIIESAKANGLNPYKYLHFIFSQLPGVLFRQHPEFLEDCL
PWSQEVQENCK

Blast result :

Comments

Genome in progress. Accession number: NZ_ACJM00000000.
ISDeal1 is 74%(orfA), 95%(orfB) and 88%(orfC) aa similar to ISCth11.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Muyzer,G. (2009) Direct submission GenBank.