ISSco1
- Family IS481
- Group
Isoform IS1649Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF099014 | ND | Streptomyces coelicolor | Streptomyces coelicolor A3 (2) |
DNA section
IS Length : 1057 bp
Ends
IR Length : 19/23
IRL : TGTGTTGTCCCGGGACGTTGGTGACACGCGTGCTGGGTTCTTGAAATGGG
IRR : TGTGTTGTTCGGAGAGGTTGGTGCCGCGGCTGGCGGGTGTGTGGCCGCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATCCAAACGACGCCC | CCTAg/aG | GCCTGCCGCGAGGCTCCC | 8 |
DNA sequence
TGTGTTGTCCCGGGACGTTGGTGACACGCGTGCTGGGTTCTTGAAATGGGTGAGGGCCTCCAGGTTCGGTGTGGATTGCGACATCTCCACCGAGTTCAGG
AGGCCCTCGTGGTCCACCGTAATGCCCCGCTGACCGAGACCGGGAGACTCCGCCTGGCCCGCTGCGTCGTCGAGGACGGATGGCCCGTGCGGCGGGCGGC
CGAGCGTTTCCAGGTCAGCCACACCACCGCCAGCCGCTGGGCCCGCCGCTACCGGCAGCTCGGCGTCACCGGAATGAGCGACCGCTCCAGCCGCCCGCAC
CACCAGCCTCGCAGGACCGCGGCCGCCGTCGAGGAGCACGTTCTCCGGCTGCGACGCGAGCACCGGATCGGGCCGCTACGACTGGCCGTCCGATGCGGCA
TCGCCGCCTCGACCGCCCACCGCATCCTGGTCCGGCACGGCCTGCCGCCACTGGCCGCCCTGGACCGGGCCACCGGCGAGCCCGTCCGCCGCTACGAACG
CGCCCGACCCGGCGAACTGGTCCACATCGACGTCAAGAAGCTGGGCCGGATCCCCGACGGCGGCGGCCACAAGACTCTCGGCCGGGCCGAAGACCGCAGC
CGGACCAACGGCGCCGGCTGGGCCTACCTGCACACCGCCCTCTACGACCACAGCCGCATCGCCTACACCGAGGACCTGCCCGACGAGACCGCCCCGACCT
GCGCTGCCTTCCTCGTTCCGGCCACGGCCTACTTCGCATCCCTGGGCATCCGCATCGAACGGGTCCTGACCGACAACGCCTGGGCCTACAGCAAGAACAC
CTGGCGGAACACCTGCCGCGACCTGGACATCAGCCCCCGCTGGACCAGGCCCTGGCGGCCCCAGACCAACGGCAAGGTCGAACGCTTCCACCGCACCCTG
CTCGACGAATGGGCCTACCAGAAGCCCTACACCTCAGACCACGAACGCAGGGAAGCGTTCACCCACTGGCTGCACTGGTACAACTACCACCGACCCCACA
CCGGCATCGGCGGCCACACACCCGCCAGCCGCGGCACCAACCTCTCCGAACAACACA
AGGCCCTCGTGGTCCACCGTAATGCCCCGCTGACCGAGACCGGGAGACTCCGCCTGGCCCGCTGCGTCGTCGAGGACGGATGGCCCGTGCGGCGGGCGGC
CGAGCGTTTCCAGGTCAGCCACACCACCGCCAGCCGCTGGGCCCGCCGCTACCGGCAGCTCGGCGTCACCGGAATGAGCGACCGCTCCAGCCGCCCGCAC
CACCAGCCTCGCAGGACCGCGGCCGCCGTCGAGGAGCACGTTCTCCGGCTGCGACGCGAGCACCGGATCGGGCCGCTACGACTGGCCGTCCGATGCGGCA
TCGCCGCCTCGACCGCCCACCGCATCCTGGTCCGGCACGGCCTGCCGCCACTGGCCGCCCTGGACCGGGCCACCGGCGAGCCCGTCCGCCGCTACGAACG
CGCCCGACCCGGCGAACTGGTCCACATCGACGTCAAGAAGCTGGGCCGGATCCCCGACGGCGGCGGCCACAAGACTCTCGGCCGGGCCGAAGACCGCAGC
CGGACCAACGGCGCCGGCTGGGCCTACCTGCACACCGCCCTCTACGACCACAGCCGCATCGCCTACACCGAGGACCTGCCCGACGAGACCGCCCCGACCT
GCGCTGCCTTCCTCGTTCCGGCCACGGCCTACTTCGCATCCCTGGGCATCCGCATCGAACGGGTCCTGACCGACAACGCCTGGGCCTACAGCAAGAACAC
CTGGCGGAACACCTGCCGCGACCTGGACATCAGCCCCCGCTGGACCAGGCCCTGGCGGCCCCAGACCAACGGCAAGGTCGAACGCTTCCACCGCACCCTG
CTCGACGAATGGGCCTACCAGAAGCCCTACACCTCAGACCACGAACGCAGGGAAGCGTTCACCCACTGGCTGCACTGGTACAACTACCACCGACCCCACA
CCGGCATCGGCGGCCACACACCCGCCAGCCGCGGCACCAACCTCTCCGAACAACACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1017 bp | 338 aa | 46 | 1062 | + | No |
Chemistry : DDE
ORF sequence :
MGEGLQVRCGLRHLHRVQEALVVHRNAPLTETGRLRLARCVVEDGWPVRRAAERFQVSHTTASRWARRYRQLGVTGMSDRSSRPHHQPRRTAAAVEEHVL
RLRREHRIGPLRLAVRCGIAASTAHRILVRHGLPPLAALDRATGEPVRRYERARPGELVHIDVKKLGRIPDGGGHKTLGRAEDRSRTNGAGWAYLHTALY
DHSRIAYTEDLPDETAPTCAAFLVPATAYFASLGIRIERVLTDNAWAYSKNTWRNTCRDLDISPRWTRPWRPQTNGKVERFHRTLLDEWAYQKPYTSDHE
RREAFTHWLHWYNYHRPHTGIGGHTPASRGTNLSEQHT
RLRREHRIGPLRLAVRCGIAASTAHRILVRHGLPPLAALDRATGEPVRRYERARPGELVHIDVKKLGRIPDGGGHKTLGRAEDRSRTNGAGWAYLHTALY
DHSRIAYTEDLPDETAPTCAAFLVPATAYFASLGIRIERVLTDNAWAYSKNTWRNTCRDLDISPRWTRPWRPQTNGKVERFHRTLLDEWAYQKPYTSDHE
RREAFTHWLHWYNYHRPHTGIGGHTPASRGTNLSEQHT
Blast result :
Comments
ISSco1 is 98% aa similar toIS1649 and IS1652, 62% to IS5564, 61% to ISAni1 and IS1121 and 59% to IS1122.
Note that the transposase termination codon is generated by sequence specific insertion.
Note that the transposase termination codon is generated by sequence specific insertion.
References
1] Chung,H.-J., Kim,E.-J., Suh,B., Choi,J.-H. and Roe,J.-H.(1999)
Gene 231, 87-93
2] Chung,H.-J., Kim,E.-J., Suh,B., Choi,J.-H. and Roe,J.-H.(1999) Direct Submission GenBank.
Gene 231, 87-93
2] Chung,H.-J., Kim,E.-J., Suh,B., Choi,J.-H. and Roe,J.-H.(1999) Direct Submission GenBank.