Tn3

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnSwi1, ISSwi1
Accession numberTranspositionOriginHost
HM769901 ND Salmonella enterica
Salmonella enterica subsp. enterica serovar Wien plasmid pZM-3
DNA section
IS Length : 4948 bp

Ends


IR Length : 38

IRL : GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
IRR : GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGCAACGTTTTCTG

Insertion site


Left flankDirect repeatRight flankDR Length
TGTGGTATGGGAAAACAAACAGCGC5

DNA sequence

GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGA
AGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTT
CATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTC
ACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGT
TGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGG
CTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAG
AAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAG
TACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAA
AAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTG
ATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGA
ATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAA
TAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCC
CTTTCGTCTTCAAGAATTTTATAAACCGTGGAGCGGGCAATACTGAGCTGATGAGCAATTTCCGTTGCACCAGTGCCCTTCTGATGAAGCGTCAGCACGA
CGTTCCTGTCCACGGTACGCCTGCGGCCAAATTTGATTCCTTTCAGCTTTGCTTCCTGTCGGCCCTCATTCGTGCGCTCTAGGATCCTCCGGCGTTCAGC
CTGTGCCACAGCCGACAGGATGGTGACCACCATTTGCCCCATATCACCGTCGGTACTGATCCCGTCGTCAATAAACCGAACCGCTACACCCTGAGCATCA
AACTCTTTTATCAGTTGGATCATGTCGGCGGTGTCGCGGCCAAGACGGTCGAGCTTCTTCACCAGAATGACATCACCTTCCTCCACCTTCATCCTCAGCA
AATCCAGCCCTTCCCGATCTGTTGAACTGCCGGATGCCTTGTCGGTAAAGATGCGGTTAGCTTTTACCCCTGCATCTTTGAGCGCTCTGATCTGAATATC
GAGGGACTGCTGGCTGGTTGAGACCCGCGCATAACCAAAAATTCGCATAAAAATGTACCTTAAATCGAATATCAGACACGATGTGTCTATTATGCCAAAA
TGACGATTTAATGGACACTCAAACGAAGCCGTTTTACTATGTCTGATAATTTATAATATTTCGAACGGTTGCAGTTGTGTTAAAAAAGCCGTCAGGCAGG
GAGGCCGATATGCCCGTTGATTTTTTGACCACTGAGCAGGTTGAGAGTTATGGCAGGTTCACTGGCGGACCCGATGAACTTCAGCTGGCGCGTTATTTTC
ATCTTGATGAAGCGGATAAAGAATTTATCGGGAAAAGCCGGGGTGATCACAATCGACTTGGTATTGCCCTGCAAATCGGGTGTGTGCGTTTTCTGGGCAC
TTTTCTTACTGACATGAATCATATTCCTTCCGGCGTCCGGCATTTTACCGCCAGACAGCTCGGGATTCGTGATATCACCGTTCTTGCAGAATACGGTCAG
AGGGAAAATACCCGCCGTGAGCATGCAGCGCTGATACGTCAGCACTATCAGTATCGTGAATTTGCCTGGCCCTGGACATTTCGCCTTACCCGTCTTTTAT
ATACCCGGAGCTGGATAAGCAACGAACGTCCTGGCCTGCTTTTCGACCTGGCGACAGGGTGGCTTATGCAACATCGTATTATTCTCCCCGGAGCCACCAC
GCTGACCCGGTTGATTTCAGAGGTAAGGGAAAAGGCGACGTTGCGCCTGTGGAACAAACTGGCACTGATACCGTCAGCCGAACAGCGTTCACAGCTGGAG
ATGCTGCTGGGGCCAACTGATTGCAGCCGCCTGTCTTTACTGGAATCACTGAAAAAAGGCCCTGTGACCATCAGTGGTCCGGCGTTTAATGAAGCAATTG
AACGCTGGAAAACTCTGAACGATTTTGGCCTGCATGCTGAAAACCTGAGTACACTCCCGGCTGTGCGCCTGAAAAATCTCGCACGTTATGCTGGTATGAC
TTCGGTGTTCAATATTGCCAGGATGTCACCGCAGAAAAGGATGGCGGTTCTGGTTGCCTTTGTCCTTGCATGGGAAACGCTGGCGCTGGATGATGCACTG
GACGTTCTGGACGCCATGCTGGCCGTTATCATCCGTGACGCCAGAAAGATTGGGCAGAAAAAACGGCTCCGCTCGCTGAAGGATCTGGATAAATCTGCAT
TGGCGCTCGCCAGCGCATGTTCGTACTTGCTGAAAGAAGAAACACCGGACGAATCGATTCGTGCTGAGGTGTTCAGCTACATCCCTAGGCAAAAGCTGGC
TGAAATCATCACGCTTGTCCGTGAAATTGCCCGGCCCTCAGACGATAATTTTCATGACGAAATGGTGGAGCAGTACGGGCGCGTTCGTCGTTTCCTGCCC
CATCTGCTGAATACCGTTAAATTTTCATCCGCACCTGCCGGGGTTACCACTCTGAATGCCTGTGACTACCTCAGCCGGGAGTTCAGCTCACGGCGGCAGT
TTTTTGACGACGCACCAACGGAAATCATCAGTCAGTCATGGAAACGGCTGGTGATTAACAAGGAAAAACATATCACCCGAAGGGGATACACGCTCTGCTT
TCTCAGTAAACTGCAGGATAGTCTGAGACGGAGGGATGTCTACGTTACCGGCAGTAACCGGTGGGGAGATCCTCGTGCAAGATTACTACAGGGTGCTGAC
TGGCAGGCAAATCGGATTAAGGTTTATCGTTCTTTGGGGCACCCGACAGACCCGCAGGAAGCAATAAAATCTCTGGGCCATCAGCTTGATAGTCGTTACA
GACAGGTTGCTGCACGTCTTGGCGAAAATGAGGCTGTCGAACTCGATGTTTCTGGCCCGAAGCCCCGGTTGACAATTTCTCCCCTCGCCAGTCTTGATGA
GCCGGACAGTCTGAAACGACTGAGCAAAATGATCAGTGATCTGCTCCCTCCGGTGGATTTAACGGAGTTGCTGCTCGAAATTAACGCCCATACCGGATTT
GCTGATGAGTTTTTCCATGCCAGTGGAGCCAGTGCCAGAGTTGATGATCTGCCCGTCAGCATCAGCGCCGTGCTGATGGCTGAAGCCTGCAATATCGGTC
TGGAACCACTGATCAGATCAAATGTTCCTGCACTGACCCGACACCGGCTGAACTGGACAAAAGCGAACTATCTGCGGGCTGAAACTATCACCAGCGCTAA
TGCCAGACTGGTTGATTTTCAGGCAACGCTGCCACTGGCACAGATATGGGGTGGAGGAGAAGTGGCATCTGCAGATGGAATGCGCTTTGTTACGCCAGTC
AGAACAATCAATGCCGGACCGAACCGCAAATACTTTGGTAATAACAGAGGGATCACCTGGTACAACTTTGTGTCCGATCAGTATTCCGGCTTTCATGGCA
TCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACGGC
AGGTGCCAGCGATCTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGAC
CATGATGCCGACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCAGGCT
CCCTGAAGCTGGGCAAAGTACAGGCCTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCCTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCG
CATCAACAAAACGCTGTATCTGCTTAATTATATTGATGATGAAGATTACCGCCGGCGCATTCTGACCCAGCTTAATCGGGGAGAAAGCCGTCATGCAGTT
GCCAGAGCCATCTGTCACGGTCAAAAAGGTGAGATAAGAAAACGATATACCGACGGTCAGGAAGATCAGTTGGGAGCTCTGGGGCTGGTCACTAACGCCG
TCGTGTTATGGAACACTATTTATATGCAGGCAGCTCTGGATCATCTCCGGGCGCAGGGTGAAACACTGAATGATGAAGATATCGCACGCCTCTCCCCGCT
TTGCCACGGACATATCAATATGCTCGGCCATTATTCCTTCACGCTGGCAGAACTGGTGACCAAAGGTCATCTGAGACCATTAAAAGAGGCGTCAGAGGCA
GAAAACGTTGCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
861 bp286 aa1008148-No
ORF function : Passenger Gene
Annotation : beta-lactamaseDescription :

ORF sequence :

MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDL
VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA
SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
558 bp185 aa17481191-No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDREGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTD
GDMGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
3006 bp1001 aa19104915+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPVDFLTTEQVESYGRFTGGPDELQLARYFHLDEADKEFIGKSRGDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQREN
TRREHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLL
GPTDCSRLSLLESLKKGPVTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVLVAFVLAWETLALDDALDVL
DAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHLL
NTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQA
NRIKVYRSLGHPTDPQEAIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMISDLLPPVDLTELLLEINAHTGFADE
FFHASGASARVDDLPVSISAVLMAEACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFVTPVRTI
NAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETGLNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDA
DYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARA
ICHGQKGEIRKRYTDGQEDQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLRPLKEASEAENV
A

 

Blast result :
Comments
Tn3 (ISSwi1) is 63% (ORF3 : the transposase) aa similar to ISThsp9.
The first ORF is a passenger gene annotated as beta-lactamase, the second is the resolvase and the third is the transposase.
This transposon disrupt the replication initiation protein gene in the plasmid pZM-3.
References
1] ISfinder submission (2012)
2] Villa L., Garcia-Fernandez A., Fortini D. and Carattoli A. (2010) J. Antimicrob. Chemother. 65 (12), 2518-2529