ISS1S
- Family IS6
- Group
Isoform Synonym(s) SK7
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| M18294 | Y | Lactococcus lactis | Lactococcus lactis subsp. lactis ML3 plasmid pSK08 |
DNA section
IS Length : 808 bp
Ends
IR Length : 18
IRL : GGTTCTGTTGCAAAGTTTTCGGATAAGTCTATTTTAGTGTAAAATGAATA
IRR : GGTTCTGTTGCAAAGTTTAAAAATCAAATACAAGGTTTATAATCCTTCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNCATTCA | ATGAAATC | ATTCTANNNN | 8 |
DNA sequence
GGTTCTGTTGCAAAGTTTTCGGATAAGTCTATTTTAGTGTAAAATGAATAAAAATGACAGCGAGGATATATCAATGAACTATTTTAAAGGTAAACAATTT
CAAAAAGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAAGAACTCCTTTATGATCGTGGGATTAACGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAAAGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGAAT
ACGCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACGAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
CAAAAAGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAAGAACTCCTTTATGATCGTGGGATTAACGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAAAGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGAAT
ACGCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACGAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 74 | 754 | + | No |
Chemistry : DDE
ORF sequence :
MNYFKGKQFQKDVIIVAVGYYLRYNLSYREIQELLYDRGINVCHTTIYRWVQEYSKVLYHLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTL
DIWLRKKRNTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIY
KKNRRNGTLFGFSVSTEIKVLMGILA
DIWLRKKRNTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMETIRGIY
KKNRRNGTLFGFSVSTEIKVLMGILA
Blast result :
Comments
ISS1S differs from ISS1T and IS946 by 4 and 27 bp, respectively. ISS1S (ISS1T) transposase shares 45 % identity with that of IS26. ISS1S and the pG+host gram+ plasmid have been derived into a thermosensitive transposition vector suitable for random insertion in Enterococcus faecalis, Streptococcus thermophilus and several lactococci (Maguin et al., 1996).
One complete and one truncated copie of an ISS1S-like element were present in Lactococcus lactis pNZ4000 respectively at coordinates 5447-5924 and 5905-6714. This element was 99% aa homologous with IS946V, 98%aa homologous with ISS1Rs and ISS1S.
One complete and one truncated copie of an ISS1S-like element were present in Lactococcus lactis pNZ4000 respectively at coordinates 5447-5924 and 5905-6714. This element was 99% aa homologous with IS946V, 98%aa homologous with ISS1Rs and ISS1S.
References