ISS1RS
- Family IS6
- Group
Isoform ISS1SSynonym(s) RS1, RS2
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X60594 | Y | Lactococcus lactis | Lactococcus plantarum 1137 Lactococcus lactis subsp. cremoris MG1363 Lactococcus lactis subsp. lactis MG1363 Lactococcus lactis subsp. lactis CNRZ270 plasmid pUCL22 Lactococcus casei 1095 Lactococcus lactis subsp. cremoris C10 Lactococcus lactis subsp. cremoris C39 Lactococcus lactis subsp. cremoris C85 Lactococcus lactis subsp. lactis 1023 Lactococcus lactis subsp. lactis L26 Lactococcus lactis subsp. lactis ML3 Lactococcus lactis subsp. lactis biovar diacetylactis D44 |
DNA section
IS Length : 808 bp
Ends
IR Length : 18
IRL : GGTTCTGTTGCAAAGTTTTCTGATAAGTCTATTTTAGTGTAAAATGAATA
IRR : GGTTCTGTTGCAAAGTTTAAAAATCAAATACAAGGTTTATAATCCTTCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNCTATCA | AGAGAATG | TCATTGNNNN | 8 |
DNA sequence
GGTTCTGTTGCAAAGTTTTCTGATAAGTCTATTTTAGTGTAAAATGAATAAAAATGACAGCGAGGATATATCAATGAACTATTTTAAAGGTAAACAATTT
CAAAAAGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAAGAACTCCTTTATGATCGTGGAATTAATGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAAAGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGAAT
ACTCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACAAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
CAAAAAGATGTGATTATTGTCGCTGTTGGTTATTATCTACGTTACAATCTAAGCTATCGTGAAATTCAAGAACTCCTTTATGATCGTGGAATTAATGTTT
GTCACACAACTATTTATCGTTGGGTACAAGAATACAGTAAAGTCCTCTATCATCTCTGGAAAAAGAAAAATAGACAGTCCTTCTATTCGTGGAAAATGGA
TGAAACTTATATCAAAATCAAAGGTCGTTGGCATTATCTCTATCGTGCAATTGATGCGGATGGATTGACTTTAGACATCTGGCTACGCAAGAAACGGAAT
ACTCAAGCTGCTTATGCGTTCTTGAAACGACTACATAAACAGTTTGGTCAACCAAGAGTAATTGTCACGGATAAAGCGCCCTCTATTGGTTCTGCATTTA
GAAAGTTACAGAGTAACGGTTTATATACTAAGACAGAGCATCGAACCGTGAAGTATCTCAATAACCTCATTGAGCAAGACCATCGACCAATCAAACGACG
CAATAAATTTTATCGAAGTCTACAAACTGCCTCAACCACGATTAAGGGCATGGAAACAATTCGAGGAATATACAAAAAGAACCGAAGAAATGGAACGCTC
TTCGGATTTTCGGTATCTACTGAGATTAAGGTCTTAATGGGAATATTAGCTTAAGAACAAGAAGGATTATAAACCTTGTATTTGATTTTTAAACTTTGCA
ACAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 74 | 754 | + | No |
Chemistry : DDE
ORF sequence :
MNKNDSEDISMNYFKGKQFQKDVIIVAVGYYLRYNLSYREIQELLYDRGINVCHTTIYRWVQEYSKVLYHLWKKKNRQSFYSWKMDETYIKIKGRWHYLY
RAIDADGLTLDIWLRKKRNTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLQTASTTI
KGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGILA
RAIDADGLTLDIWLRKKRNTQAAYAFLKRLHKQFGQPRVIVTDKAPSIGSAFRKLQSNGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYRSLQTASTTI
KGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGILA
Blast result :
Comments
ISS1RS differs from ISS1T by 1 nt (1 aa). The distribution of ISS1RS among Lactococcus species parallels that of the lactose plasmid (Bounaix et al., 1996). ISS1RS is present in one copy in the 2,560 kb chromosome of the Lactococcus lactis subsp. cremoris reference strain MG1363 (Le Bourgeois et al., 1995).
References