ISS1M
- Family IS6
- Group
Isoform ISS1NSynonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X59946 | ND | Lactococcus lactis | Lactococcus lactis subsp. cremoris SK11 plasmid pSK111 |
DNA section
IS Length : 808 bp
Ends
IR Length : 16/18
IRL : AGTTCTGTTGCAAAGTTTTCCAAAAAATCTATTTTAGTGTAAAACTGAGA
IRR : GGTTCTGTTGCGAAGTTTGAAAATCAAACGCGCCCGCTCTGAACTCCAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
AGTTCTGTTGCAAAGTTTTCCAAAAAATCTATTTTAGTGTAAAACTGAGAAAAAAGACAGAGAGGACAGAGGAATGAATCATTTTAAAGGCAAACAATTC
AAAAAAGACTTCATTATTGTCGCTGTTGGTTACTACCTGCGTTACAATCTAAGCTATCGTGAAGTTCAGAAATTGTTATATGATCGTGGAATAAATGTTT
GTCATACTACGATTTATCGTTGGGTGCAAGAGTACAGCAAAGTCCTCTATGATCTTTGGAAGAAGAAAAATAGACAATCCTTCTATTCATGGAAAATGGA
CGAAACCTATATCAAAATTAAGGGACGTTGGCATTATCTTTATCGTGCAATTGATGCGGACGGCTTAACCTTAGATATCTGGTTACGAAAGAAACGGGAG
ACGCAAGCAGCCTATGCTTTCTTAAAACGACTCCATAAACAGTTTGGTGAGCCGAAAGCAATTGTGACCGATAAAGCACCTTCTCTTGGCTCCGCCTTTA
TAAAGTTACAGAGTGTGGGTTTATATACTAAGACAGAGCACCGAACTGTGAAGTATCTTAACAATTTAATAGAACAAGACCATCGACCTATTAAACGACG
GATTAAATTTTATCAAAGTCTCCGTACAGCCTCTTCCACGATTAAGGGCATGGAGACCCTTCGAGGAATATATAAAAATAACCGAAGAAATGGAACGCTC
TTCGGCTTTTCGGTGTCTACTGAAATCAAGGTATTAATGGGAATAACAGCCTAAGATATTTGGAGTTCAGAGCGGGCGCGTTTGATTTTCAAACTTCGCA
ACAGAACC
AAAAAAGACTTCATTATTGTCGCTGTTGGTTACTACCTGCGTTACAATCTAAGCTATCGTGAAGTTCAGAAATTGTTATATGATCGTGGAATAAATGTTT
GTCATACTACGATTTATCGTTGGGTGCAAGAGTACAGCAAAGTCCTCTATGATCTTTGGAAGAAGAAAAATAGACAATCCTTCTATTCATGGAAAATGGA
CGAAACCTATATCAAAATTAAGGGACGTTGGCATTATCTTTATCGTGCAATTGATGCGGACGGCTTAACCTTAGATATCTGGTTACGAAAGAAACGGGAG
ACGCAAGCAGCCTATGCTTTCTTAAAACGACTCCATAAACAGTTTGGTGAGCCGAAAGCAATTGTGACCGATAAAGCACCTTCTCTTGGCTCCGCCTTTA
TAAAGTTACAGAGTGTGGGTTTATATACTAAGACAGAGCACCGAACTGTGAAGTATCTTAACAATTTAATAGAACAAGACCATCGACCTATTAAACGACG
GATTAAATTTTATCAAAGTCTCCGTACAGCCTCTTCCACGATTAAGGGCATGGAGACCCTTCGAGGAATATATAAAAATAACCGAAGAAATGGAACGCTC
TTCGGCTTTTCGGTGTCTACTGAAATCAAGGTATTAATGGGAATAACAGCCTAAGATATTTGGAGTTCAGAGCGGGCGCGTTTGATTTTCAAACTTCGCA
ACAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 681 bp | 226 aa | 74 | 754 | + | No |
Chemistry : DDE
ORF sequence :
MNHFKGKQFKKDFIIVAVGYYLRYNLSYREVQKLLYDRGINVCHTTIYRWVQEYSKVLYDLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTL
DIWLRKKRETQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFIKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRIKFYQSLRTASSTIKGMETLRGIY
KNNRRNGTLFGFSVSTEIKVLMGITA
DIWLRKKRETQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFIKLQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRIKFYQSLRTASSTIKGMETLRGIY
KNNRRNGTLFGFSVSTEIKVLMGITA
Blast result :
Comments
ISS1M and ISS1N transposases are 97 % identical.
References
1] de Vos, W.M., Boerrigter, I., Vos, P., Bruinenberg, P., and Siezen R.J. (1991) "Production, processing, and engineering of the Lactococcus lactis SK11 proteinase" in Dunny, G.M., Clearly, P.P., and McKay, L.L. (eds.) Genetics and Molecular Biology of Streptococci, Lactococci and Enterococci, pp. 115-119 American Society for Microbiology, Washington.