ISRsp1

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISRm8
Accession numberTranspositionOriginHost
U00090 ND Rhizobium sp.
Rhizobium sp. NGR234
Rhizobium meliloti 41 plasmid pSym-a
Rhizobium sp. NGR234 plasmid pNGR234a
DNA section
IS Length : 3481 bp

Ends


IR Length : 17/22

IRL : GTATCCGTCCGAGCGCGACCGCCTTTGCATATTGAGTAAAACTGTTCAGA
IRR : GTATCCATCCGAGGTGGGCCGCGGGACTTGGGGGTCTCGCGCGTGGTAGG

Insertion site


Left flankDirect repeatRight flankDR Length
AGGCGCGCCAGATAAGGGCACTCGTCTC8
GCCTTCTGCATTCGGATGCACGAGGGCG8

DNA sequence

GTATCCGTCCGAGCGCGACCGCCTTTGCATATTGAGTAAAACTGTTCAGACCCTGTCCTTATTCCCATGCATAAGGACAGTTGCCAACATGGAGATTTTT
GAAGGAGACAGCGGGTCTCGGGTGAGCCGTCTTGAGGTGATCAACACCGGACGGCGGCGTCGATTTACGGAAGATGAGAAGCTGCGGATTGTCGCAGAAA
GCTTTGCCGGGAGAGGCCGTGCGTCGGCCACGGCCCGTCAGTACGGCATCAGTCGCTCCCTGTTGAACCGTTGGCGCAAATCGGTTCGCCAGGGTTTGCA
CGGCCAGAAACAAACCGATGGTTTTGTGCCGGCGTTCGTCATGCCGGAAACTTTTGTGCCGGTGAAGCAGGTCACTCCACCTGCTGCGATGGAGCAGCCG
GTGGCGTCTCCTTCCGGCCGCATGGAGATTGTTGCGGCGAACGGCCGTCGTGTGGTCGTGGACGGCAGCGTCGACGTTGAGGCGCTGCTGCGGATCATGC
GGGGGCTGGAGACGTTGCGGTGATCATGCTTCCTTCCGGTCAAAATGTGCGGGTGTGGATTGCAACGGGCCATACGGACATGCGGTGTGGGTTTCCATCG
CTTGCGTTGCGGGTGCAGGAGGTGCTGAAACTGAACCCTTTGGACGGCAATCTTTTTGTGTTTCGCGGTCGCAGCGGATCGCTGCTAAAAGTGATCTGGA
GTGACGGCCAGGGGAGCTGCCTTTTTACAAAAAGATTGGACCGTGGCCGGTTCGTCTGGCCTTCTGCCGAAGGCGGAGCGATAGCGATATCACCCGCGCA
GCTCAGTTATCTTCTGTCCGGAATCGACTGGAGGCATCCTCAGGAAACCTGGCGGCCGACGAAGGTCGGCTAGCATTATTCTATTGAAAATACAGGGGAA
ATCTGATCGAATGGCTTCATGATCTCAAAGCCTGTCGATCTTCCTGTGGATGTTGTTGGCGCTTACCTGGCGCTGCGTGGCGAGCATGAAGCCTTGCAGG
CTAAACACGCTATCGCAGTAGCGGAAGCCGCCAATGCGCAGGCGATGCTCTCTGACAACGAGGCGCTGATCGTTGCTCTGGAATTGAAGATCGAGAAGCT
CAGGCGCGAGTTGCGGGGCCAGCGCTCTGAGCGCACGGCGCGCCTGCTCGACCAGTTGGAACTGCAGCTCGAGGAACTCGTGGCGGCGGCGACGGAGGAT
GAGGTCGCGGCACAAGCAGCAAGCGCCAGAACCTCGAGCGTACGTTCGTTCACGCGCAAACGGCCGGTGCGCAAACCATGGCCGGACGATATCGAGCGCG
AACGTGTTGTCATCGAGCCGCCGACGACTTGCACCTGCTGTGGTGGTTCGCGCCTGTCGAAGCTGGGCGAGGACGTCACCGAGACGCTGGAAGAGATCCC
ACGCCGGTTCAAAGTGATCGAGACGGTGCGGGAAAAGTTCACCTGCCGCGACTGTGAGGCGATCAGTCAGACGCCCGCACCCTTCCATGCCACGCCGCGC
GGCTTTATCGGGCCGAACCTGCTGGCGACGATCCTGTTCGACAAGTTCGGCATGCACAGCCCGCTCAACCGGCAGAGTGCCCGGTTCAAATGCGAGGGGA
TCGATCTTTCGACCTCGACGCTGGCCGACCAGGTCGGGTACGCAACAGCCGCTCTCATGCCTGTCTTCGATCTGATCGAGGCGCATGTCTTCGCGGCCGA
GCGTCTTCACGGTGATGACACCACCATTCCCATTCAGGCCAGGGACAAATGCACGACCGGACGCATATGGACTTACGTATGCGATGACCGGCCATTCGGG
GGAACGGCGCCGCCAGCCGCAATCTATTATGCGTCGAGTGACCGGCGCGGCGAACATCCGCAGAAACACCTGGCCGGATACGGCGGCATTCTGCAGAGCG
ATTGCTACAATGGCTTCGAGCCGATCGCTGTTGCCGCAACGAAAGCGGTCCCGATCACATTCGCCTTTTGTCACGCGCATGCGCGGCGGAAATTCTTTGA
GCTGGCCGATATCCAGAAGAATGCGCGGGATCGCAAACGGAGGGGCAAGCCGATCTCGCCGATCGCATTGGAAGCCGTCAAGCGCTACGACGAATTGTTC
GAGATCGAGCGCCAGATCAACGGATTGAGCGCCGAAGAACGACTGGCTGTGCGGCAGGAGAAGAGCAAGCCACTGTTCGATGACATGCACGAGTGGTTGA
CGAAGGAACGCGCCATGCTCAGCAGATCGTCCGAGGTCATCGAGCCGATCGATTACATGCTCAAGCGCTGGGAGGGCTTTGCTCTCTTCCTCAAAGACGG
GAGAGTTTGTTTAACGAACAACGCAGCCGAGCGGGCGCTGAGAAGTGTCGCATTGGGAAGACGGAACTGGACCTTCGCCGGTTCTCAGCGCGGGGCCGAT
CGTGCCGCTGTCATGCTGACCGTCATCACCACCTGCCGCCTCAACGATATCGACCCGAAGGCATGGCTTGCAGACGTGCTGGCTCGCATCGCCGACCATC
CTGTCACGCGCCTGTACGAACTGCTGCCCTGGGAGTGGAAACGTGCATCGGCGGCAACCGTCATGCTGGCGGCCTGACGATGGTGGCTTCCGTCGTCCGC
CTGAAGGTCACCCTCGATCATGTCGAGCCGATAGTGATGCGGCGCGTTGTCGTGCCGTTCACCATCAGGCTCAGTCGGCTGCACGAGGTGTTGCAGGCGG
CGATGGGCTGGACCAACAGCCACCTTTACGAATTCCGCATGCGTGACGTCGGCTTTGGTTTGCCTGACGAGGAATGGGGGGATGGTCCGATCGATGCCCG
CAGGGTCTCGCTGCTGTCGGCAGTCCAGGACACCGGTGCGAAATCGTTCAAATACCTTTACGACTTTGGCGACGGCTGGGAACACAGCATCAAGATCGAG
CGCACCTTTCCTGCGGTCGGCACGGAAGGACCGATGCTTCTCGAAGCAACGGGGCACTGCCCGCCCGAAGACGTTGGTGGTCCATGGGGTTATCAGGAGT
TCTGCGAGGCGCTTGCAGACCCGGCGCACGAGCGACATGCCGAAACCCTCGAATGGTGCGGCAGCAGCGATTATGATTCCGCCGCCGCCAACTTCTCGCA
GCTCAACAAAGCCGTCGATGACCTCGCTGCAAAATGGGCCCGAAAAGCGCGCCGCAAAACCTGAACGCCGGGCTGTGGCCGAAGGCCGAAAGTTGATTTT
ACCGTGTAAACAACCGGCGGAACAGTTCTTCCGAGCGCCGTAGACCTTCGTCCGTCAGCACCAGCGACTTCGACTTGTTCACCGGATCACAGATCAGTCC
CCTCTCATGCAACCGGTCCGTCGTCGCCCAGTCAAATCCCTTCCAGGCGCAACGCTCGTTGTGGAGCGTCAGCCATAACAGTGCCAGCACCGCGTCATCG
ATTTTGTCCTCGTCGATCTCCATTCTGCTATCCTACCACGCGCGAGACCCCCAAGTCCCGCGGCCCACCTCGGATGGATAC
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
435 bp144 aa89523+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MEIFEGDSGSRVSRLEVINTGRRRRFTEDEKLRIVAESFAGRGRASATARQYGISRSLLNRWRKSVRQGLHGQKQTDGFVPAFVMPETFVPVKQVTPPAA
MEQPVASPSGRMEIVAANGRRVVVDGSVDVEALLRIMRGLETLR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
348 bp115 aa526873+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MLPSGQNVRVWIATGHTDMRCGFPSLALRVQEVLKLNPLDGNLFVFRGRSGSLLKVIWSDGQGSCLFTKRLDRGRFVWPSAEGGAIAISPAQLSYLLSGI
DWRHPQETWRPTKVG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1659 bp552 aa9192577+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISKPVDLPVDVVGAYLALRGEHEALQAKHAIAVAEAANAQAMLSDNEALIVALELKIEKLRRELRGQRSERTARLLDQLELQLEELVAAATEDEVAAQA
ASARTSSVRSFTRKRPVRKPWPDDIERERVVIEPPTTCTCCGGSRLSKLGEDVTETLEEIPRRFKVIETVREKFTCRDCEAISQTPAPFHATPRGFIGPN
LLATILFDKFGMHSPLNRQSARFKCEGIDLSTSTLADQVGYATAALMPVFDLIEAHVFAAERLHGDDTTIPIQARDKCTTGRIWTYVCDDRPFGGTAPPA
AIYYASSDRRGEHPQKHLAGYGGILQSDCYNGFEPIAVAATKAVPITFAFCHAHARRKFFELADIQKNARDRKRRGKPISPIALEAVKRYDELFEIERQI
NGLSAEERLAVRQEKSKPLFDDMHEWLTKERAMLSRSSEVIEPIDYMLKRWEGFALFLKDGRVCLTNNAAERALRSVALGRRNWTFAGSQRGADRAAVML
TVITTCRLNDIDPKAWLADVLARIADHPVTRLYELLPWEWKRASAATVMLAA

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
585 bp204 aa25803164+No
ORF function : Passenger Gene
Annotation : pRiA4b ORF-3 family protein Description :

ORF sequence :

MVASVVRLKVTLDHVEPIVMRRVVVPFTIRLSRLHEVLQAAMGWTNSHLYEFRMRDVGFGLPDEEWGDGPIDARRVSLLSAVQDTGAKSFKYLYDFGDGW
EHSIKIERTFPAVGTEGPMLLEATGHCPPEDVGGPWGYQEFCEALADPAHERHAETLEWCGSSDYDSAAANFSQLNKAVDDLAAKWARKARRKT

 

Blast result :
Comments
ISRsp1 is 51%(orfA) aa similar to ISAli10, 80%(orfB) and 77%(orfC, Transposase) to IS66, and 62%(orfD) to ISBrsp6.
ISRm8, partial IS, is 81 % identical to ISRsp1 (1002-1785), including a gap of 42 bp around position 380. The same region is also similar to IS66 (66 %, 855-1619). ISRm8 is located downstream of the nodQ1 (APS kinase) gene.
References
1] Freiberg,C., Fellay,R., Bairoch,A., Broughton,W.J., Rosenthal,A. and Perret,X. (1997) Nature 387 (6631), 394-401.
2] Freiberg,C. (1996) Direct submission GenBank.
3] Schwedock, J., and Long, S.R. (1994) Mol. Plant-Microbe Interact. 7, 151-153.