ISRso17
- Family IS701
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003295 | ND | Ralstonia solanacearum | Ralstonia solanacearum |
DNA section
IS Length : 1471 bp
Ends
IR Length : 16/17
IRL : TCTTACTGTGTCATAAAAAGTAGCGCATTATATGTACTTCCTAATTCAAG
IRR : TCTTACTGTGTCACAAATGTGATTGATCAGCCCGACGCGCAGCATGGGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGCTGGCGC | CTAT | GAAGACGCGC | 4 |
DNA sequence
TCTTACTGTGTCATAAAAAGTAGCGCATTATATGTACTTCCTAATTCAAGGTGGGGAGTGCATGAGCGCAAGCGACCGATTTGCGGAGTACATGAACTAC
TTGGCTGAGGGATTGGGGCATGCAGATCGCCGGGCCGGCTTGGAGAGCTATTGCACTGGCCTGATGCTGCCGCTGTCGCGCAAGAGCGTCGAACCGATGG
CGGCACGGGTTGACCCGTTGCATGCCAGTGCTCGGCATCAGTCACTGCACCACTTCGTCTCCAAATCCGATTGGTCGGATACGGCGTTGTTAAGCCGTGT
CCGGGACTGGGTTCAGCCCTTGCTGGAAGCGCCAAGCGGTCGCTATTGGATCATTGACGACACGGGTTTCCCTAAGAAAGGCAAACATTCGGTTGGCGTG
GCACGCCAATATTGCGGCCAGCTTGGCAAACAGGACAACTGCCAGGTGGCAGTCAGCCTGTCGCTGGCCACAGAGCAAGGCAGCCTTCCGATTGCCTATC
AGCTTTATCTCCCCAAGGAATGGGCCGACGATGCGACTCGCCGCCAGAAGGCCGGCGTGCCTGACGATATCGTGTTCGCCACCAAGCCGGAGATTGCCCT
GGCGCAAGTGCGCGAGGCGATCGCAGCCGGGGTGCCAGCCGGAGTCGTGCTGGCCGACGCTGCATATGGCGATGAAACCGGGTTTCGCGAAAATCTGACC
GAGTTGGGTCTGCTTTACGCCGTCGGGATTCGCTCCACGACGACGGTGTGGCCGCCGGGCGTTTCGCCACTGCCGGCCAAACCGGCGAGCGGTCCGGGTA
GGCCACCGAAGTTGTTGCGTCGCGCACCGGGCCATGAGCCGGTTTCAGTGAAGACCCTGGCGATGGCATTACCCTCGAGCGCTTACCAAGTGGTGACTTG
GCGGGAAGGCATCAATACAGCCCTTTCATCGCGATTTGCGGCCGTGCGGGTTCGCCCGGCGCATCGGGACTATTGGCGCAGCACCTTGCGCGACGAAGAA
TGGTTGCTGGTTGAGTGGCCGGGGGGCGAAGCGGAGCCAACCAAATACTTCTTGTCCACAGCACCAGCCGATGCGCTGATAGAACAACTGGTGTTCGTGA
CCAAAATGCGTTGGCGTATTGAGCGCGATTACCAGGACTTGAAACAAGAGCTCGGCCTTGGTCACTACGAAGGCCGGGGCTGGCGCGGATTCCACCATCA
TGCGACGCTGAGTATCGCAGCCTATGGCTTCCTGATGGCCGAACGACTCGCTGCAGACAAGCCCGTGGGGAATAAAAAAAACTTCCTCGAATGCCAGATA
CCTGCGCTTCCCGCGGATTACATCCCCCGTGGCAGCCCTGCGTGCGCAGCGACACGTGACGAATTCAATCCCCACACTCCGTCATCAACTTAGCTACCAC
CTGATGCGCAGCCTTGGGCTTTGCCCATGCTGCGCGTCGGGCTGATCAATCACATTTGTGACACAGTAAGA
TTGGCTGAGGGATTGGGGCATGCAGATCGCCGGGCCGGCTTGGAGAGCTATTGCACTGGCCTGATGCTGCCGCTGTCGCGCAAGAGCGTCGAACCGATGG
CGGCACGGGTTGACCCGTTGCATGCCAGTGCTCGGCATCAGTCACTGCACCACTTCGTCTCCAAATCCGATTGGTCGGATACGGCGTTGTTAAGCCGTGT
CCGGGACTGGGTTCAGCCCTTGCTGGAAGCGCCAAGCGGTCGCTATTGGATCATTGACGACACGGGTTTCCCTAAGAAAGGCAAACATTCGGTTGGCGTG
GCACGCCAATATTGCGGCCAGCTTGGCAAACAGGACAACTGCCAGGTGGCAGTCAGCCTGTCGCTGGCCACAGAGCAAGGCAGCCTTCCGATTGCCTATC
AGCTTTATCTCCCCAAGGAATGGGCCGACGATGCGACTCGCCGCCAGAAGGCCGGCGTGCCTGACGATATCGTGTTCGCCACCAAGCCGGAGATTGCCCT
GGCGCAAGTGCGCGAGGCGATCGCAGCCGGGGTGCCAGCCGGAGTCGTGCTGGCCGACGCTGCATATGGCGATGAAACCGGGTTTCGCGAAAATCTGACC
GAGTTGGGTCTGCTTTACGCCGTCGGGATTCGCTCCACGACGACGGTGTGGCCGCCGGGCGTTTCGCCACTGCCGGCCAAACCGGCGAGCGGTCCGGGTA
GGCCACCGAAGTTGTTGCGTCGCGCACCGGGCCATGAGCCGGTTTCAGTGAAGACCCTGGCGATGGCATTACCCTCGAGCGCTTACCAAGTGGTGACTTG
GCGGGAAGGCATCAATACAGCCCTTTCATCGCGATTTGCGGCCGTGCGGGTTCGCCCGGCGCATCGGGACTATTGGCGCAGCACCTTGCGCGACGAAGAA
TGGTTGCTGGTTGAGTGGCCGGGGGGCGAAGCGGAGCCAACCAAATACTTCTTGTCCACAGCACCAGCCGATGCGCTGATAGAACAACTGGTGTTCGTGA
CCAAAATGCGTTGGCGTATTGAGCGCGATTACCAGGACTTGAAACAAGAGCTCGGCCTTGGTCACTACGAAGGCCGGGGCTGGCGCGGATTCCACCATCA
TGCGACGCTGAGTATCGCAGCCTATGGCTTCCTGATGGCCGAACGACTCGCTGCAGACAAGCCCGTGGGGAATAAAAAAAACTTCCTCGAATGCCAGATA
CCTGCGCTTCCCGCGGATTACATCCCCCGTGGCAGCCCTGCGTGCGCAGCGACACGTGACGAATTCAATCCCCACACTCCGTCATCAACTTAGCTACCAC
CTGATGCGCAGCCTTGGGCTTTGCCCATGCTGCGCGTCGGGCTGATCAATCACATTTGTGACACAGTAAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1332 bp | 443 aa | 62 | 1393 | + | No |
Chemistry : DDE
ORF sequence :
MSASDRFAEYMNYLAEGLGHADRRAGLESYCTGLMLPLSRKSVEPMAARVDPLHASARHQSLHHFVSKSDWSDTALLSRVRDWVQPLLEAPSGRYWIIDD
TGFPKKGKHSVGVARQYCGQLGKQDNCQVAVSLSLATEQGSLPIAYQLYLPKEWADDATRRQKAGVPDDIVFATKPEIALAQVREAIAAGVPAGVVLADA
AYGDETGFRENLTELGLLYAVGIRSTTTVWPPGVSPLPAKPASGPGRPPKLLRRAPGHEPVSVKTLAMALPSSAYQVVTWREGINTALSSRFAAVRVRPA
HRDYWRSTLRDEEWLLVEWPGGEAEPTKYFLSTAPADALIEQLVFVTKMRWRIERDYQDLKQELGLGHYEGRGWRGFHHHATLSIAAYGFLMAERLAADK
PVGNKKNFLECQIPALPADYIPRGSPACAATRDEFNPHTPSST
TGFPKKGKHSVGVARQYCGQLGKQDNCQVAVSLSLATEQGSLPIAYQLYLPKEWADDATRRQKAGVPDDIVFATKPEIALAQVREAIAAGVPAGVVLADA
AYGDETGFRENLTELGLLYAVGIRSTTTVWPPGVSPLPAKPASGPGRPPKLLRRAPGHEPVSVKTLAMALPSSAYQVVTWREGINTALSSRFAAVRVRPA
HRDYWRSTLRDEEWLLVEWPGGEAEPTKYFLSTAPADALIEQLVFVTKMRWRIERDYQDLKQELGLGHYEGRGWRGFHHHATLSIAAYGFLMAERLAADK
PVGNKKNFLECQIPALPADYIPRGSPACAATRDEFNPHTPSST
Blast result :
Comments
There are 7 copies of ISRso17 on Ralstonia solanacearum genome (7 on megaplasmid).One copie is disrupted by ISRso9.
References
1] Salanoubat,M., Genin,S., Artiguenave,F., Gouzy,J., Mangenot,S.,Arlat,M., Billault,A., Brottier,P., Camus,J.C., Cattolico,L., Chandler,M., Choisne,N., Claudel-Renard,C., Cunnac,S., Demange,N., Gaspin,C., Lavie,M., Moisan,A., Robert,C., Saurin,W., Schiex,T., Siguier,P., Thebault,P., Whalen,M., Wincker,P., Levy,M., Weissenbach,J. and Boucher,C.A. (2002) Nature 415 (6871), 497-502