ISRso12
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003295 | ND | Ralstonia solanacearum | Ralstonia solanacearum |
DNA section
IS Length : 1225 bp
Ends
IR Length : 11/12
IRL : TGACCTCCCCCCGAAATATTGGACACGCCAGAACAGGAGAAAACCGGCGA
IRR : TGACCTCCTCCCAAGAACTGGACAGGGTCAGGCTAGAGAAGCCGGCGTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGTCCAGTCG | ATGC | CTGTTCATGT | 4 |
DNA sequence
TGACCTCCCCCCGAAATATTGGACACGCCAGAACAGGAGAAAACCGGCGACACTGCCCTTGAAGGAGGGAGTTCGCCATGAAGAAGTCGAAGTTCACGGA
AGAGCAGATTGCGTTCGCGCTGCATCAGGCCGAGACAGGTGTCTCGGTCGAAGAGGTATGCCGCAAGATTGGCATCTCGCAAGCGACGTTTTACGCGTGG
AAGAAGAAGTACGGCGGTATTGGCGTGAGCGAGCTACGGCGGTTGCGTCAACTTGAAGAGGAGAACGCCAAGCTGCGGCGCTTGGTGGCCGACTTGAGCC
TGGACAAGGTCATGCTGCAGGATGTTCTGAGAAAAAAGCTCTAAGGCCGGCGCAGCGCAAAGCGCTCGTCGGCCATCTCATCGAACGGTACCGCGTCAGC
ATTAAACGCGCCTGCCAGGTCTGCCGGCAAAGCCGCGCAGGTTGGTACCAGAAGCCGAAACCGAGGCTGCTGGACGCACCGATCAAGGCCCGCATGCACG
AGATCGCCAGCACCCGGGTACGGTACGGCTTCTGGCGCATTTACGTGCTGATTCGTCGCGAGGGTTGGTTGGTCAATCACAAACGCGTCTACCGCCTGTA
CAAGGAGGAAGGCTTGAATCTGCGCACCAAAAGACCCAGGCGCCGCAAGGCGGCCGCACATAGACTCGAGCGCCCGGTGCTCACCGCACCGAATCAGAGC
TGGAGCATGGATTTCGTATCCGACGCCTTGTTTAACGGCAACAAGTTCCGCGCACTGACTGTGGTGGACAACCATACGCGGGAATGCCTGGCGATTGAGG
CTGCCCCGTCGCTAAACGGCAGTGACGTTGTGCGTGTGTTGCAAGCCATCGTGATCCGACGAGGCATGGTGCCCGCACGCATCCAGGCTGACAACGGGCC
GGAATTTGTGTCGCTGGCGCTGGACAAGTGGGCGTATGAGGCAGGCGTAACGCTCGACTTCTCTCGGCCTGGCAAGCCTACCGACAATGCCTTCATCGAG
TCCTTCAACGGCAGCTTGCGCGATGAATGCCTGAACACGTACTGGTTCCTGTCCCTTGAAGACGCACGCCAAAAGCTCGAAAACTGGAGGCACGATTACA
ATCATTTCCGGCCACATTCGGCGCTGGATGACACCGCACCAGCGCTCTTTGCACGGCAGTTTGCCATTACACCAACCACGCCGGCTTCTCTAGCCTGACC
CTGTCCAGTTCTTGGGAGGAGGTCA
AGAGCAGATTGCGTTCGCGCTGCATCAGGCCGAGACAGGTGTCTCGGTCGAAGAGGTATGCCGCAAGATTGGCATCTCGCAAGCGACGTTTTACGCGTGG
AAGAAGAAGTACGGCGGTATTGGCGTGAGCGAGCTACGGCGGTTGCGTCAACTTGAAGAGGAGAACGCCAAGCTGCGGCGCTTGGTGGCCGACTTGAGCC
TGGACAAGGTCATGCTGCAGGATGTTCTGAGAAAAAAGCTCTAAGGCCGGCGCAGCGCAAAGCGCTCGTCGGCCATCTCATCGAACGGTACCGCGTCAGC
ATTAAACGCGCCTGCCAGGTCTGCCGGCAAAGCCGCGCAGGTTGGTACCAGAAGCCGAAACCGAGGCTGCTGGACGCACCGATCAAGGCCCGCATGCACG
AGATCGCCAGCACCCGGGTACGGTACGGCTTCTGGCGCATTTACGTGCTGATTCGTCGCGAGGGTTGGTTGGTCAATCACAAACGCGTCTACCGCCTGTA
CAAGGAGGAAGGCTTGAATCTGCGCACCAAAAGACCCAGGCGCCGCAAGGCGGCCGCACATAGACTCGAGCGCCCGGTGCTCACCGCACCGAATCAGAGC
TGGAGCATGGATTTCGTATCCGACGCCTTGTTTAACGGCAACAAGTTCCGCGCACTGACTGTGGTGGACAACCATACGCGGGAATGCCTGGCGATTGAGG
CTGCCCCGTCGCTAAACGGCAGTGACGTTGTGCGTGTGTTGCAAGCCATCGTGATCCGACGAGGCATGGTGCCCGCACGCATCCAGGCTGACAACGGGCC
GGAATTTGTGTCGCTGGCGCTGGACAAGTGGGCGTATGAGGCAGGCGTAACGCTCGACTTCTCTCGGCCTGGCAAGCCTACCGACAATGCCTTCATCGAG
TCCTTCAACGGCAGCTTGCGCGATGAATGCCTGAACACGTACTGGTTCCTGTCCCTTGAAGACGCACGCCAAAAGCTCGAAAACTGGAGGCACGATTACA
ATCATTTCCGGCCACATTCGGCGCTGGATGACACCGCACCAGCGCTCTTTGCACGGCAGTTTGCCATTACACCAACCACGCCGGCTTCTCTAGCCTGACC
CTGTCCAGTTCTTGGGAGGAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 78 | 344 | + | No |
Description : First part of the transposase
ORF sequence :
MKKSKFTEEQIAFALHQAETGVSVEEVCRKIGISQATFYAWKKKYGGIGVSELRRLRQLEEENAKLRRLVADLSLDKVMLQDVLRKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 942 bp | 313 aa | 257 | 1198 | + | No |
Description : Second part of the transposase
ORF sequence :
RGERQAAALGGRLEPGQGHAAGCSEKKALRPAQRKALVGHLIERYRVSIKRACQVCRQSRAGWYQKPKPRLLDAPIKARMHEIASTRVRYGFWRIYVLIR
REGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERPVLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVI
RRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGKPTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFAR
QFAITPTTPASLA
REGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERPVLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVI
RRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGKPTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFAR
QFAITPTTPASLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1121 bp | 373 aa | 78 | 1198 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKSKFTEEQIAFALHQAETGVSVEEVCRKIGISQATFYAWKKKYGGIGVSELRRLRQLEEENAKLRRLVADLSLDKVMLQDVLRKKALRPAQRKALVGH
LIERYRVSIKRACQVCRQSRAGWYQKPKPRLLDAPIKARMHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERP
VLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVIRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGK
PTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA
LIERYRVSIKRACQVCRQSRAGWYQKPKPRLLDAPIKARMHEIASTRVRYGFWRIYVLIRREGWLVNHKRVYRLYKEEGLNLRTKRPRRRKAAAHRLERP
VLTAPNQSWSMDFVSDALFNGNKFRALTVVDNHTRECLAIEAAPSLNGSDVVRVLQAIVIRRGMVPARIQADNGPEFVSLALDKWAYEAGVTLDFSRPGK
PTDNAFIESFNGSLRDECLNTYWFLSLEDARQKLENWRHDYNHFRPHSALDDTAPALFARQFAITPTTPASLA
Blast result :
Comments
There are 3 copies of ISRso12 on Ralstonia solanacearum genome (2 on chromosome and 1 on megaplasmid).
References
1] Salanoubat,M., Genin,S., Artiguenave,F., Gouzy,J., Mangenot,S.,Arlat,M., Billault,A., Brottier,P., Camus,J.C., Cattolico,L., Chandler,M., Choisne,N., Claudel-Renard,C., Cunnac,S., Demange,N., Gaspin,C., Lavie,M., Moisan,A., Robert,C., Saurin,W., Schiex,T., Siguier,P., Thebault,P., Whalen,M., Wincker,P., Levy,M., Weissenbach,J. and Boucher,C.A. (2002) Nature 415 (6871), 497-502