ISRso10

  • Family IS3
  • Group IS2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003295 ND Ralstonia solanacearum
Ralstonia solanacearum
DNA section
IS Length : 1335 bp

Ends


IR Length : 22/28

IRL : TGGAACGGCCCCCAGAATCCCCGGACACAGTCACCCACTTAAACTACGGG
IRR : TGGATTTGCCCCCGCATCTCCGGACACCGCCTCACACTTGAGTTGATGAT

Insertion site


Left flankDirect repeatRight flankDR Length
CGGCAACGAGAGCTGAATCAACAAC5

DNA sequence

TGGAACGGCCCCCAGAATCCCCGGACACAGTCACCCACTTAAACTACGGGTAGGCATACGACTGTGTTTATGACTAGCAGCAACCAAGAAGTGATGGAAG
TGTTGACGGGCCCGGAGCGTCGCCGGCGGTGGTCGGTGGAAGAGAAGCTTGCGATGGTCCGAGAGAGCTTTGAGCCGGGCAAGACGGTCTCGATGGTGGC
GCGCCAGCACGGCGTGAATCCGAATCAGGTGTTCCAGTGGCGCAAGCTGTACCAAGACGGGAGCCTGTCGGCAGTGACCGCGGGCGAGGAAGTGGTGCCG
GCCTCGGAGCTGGCCGAAGCGCTCAAACAAGTCCGTGAGCTGCAGCGCATGCTCGGCAAGAAGACGATGGAGAACGAGATTCTTCGCGAAGCCGTGGAAT
ACGGTCGGGCAAAAAAGTGGATAGCGCGCTCGCCATCGTTGCCGGGGGACGACCAGTGAAGCTAGTTTGCGAGGTCCTCGGCGTAGCGCGCTCAAACGTG
GCGGCCAAGCGGGCCCGGCCGGGTGATTGGCGCGACGGGCGCACAGCTCGGCAAACCGACGACGCTAGCTTGGTCGACGAAATCCGGCGCACCATCGCCG
ACTTGCCGAGCTATGGCTACCGGCGGGTCTGGGGCAGATTGCGGCGCGAACGTGAGCGTCAATGCGAAGCGCCGATCAATGCCAAGCGTGTCTATCGGGT
CATGCGTGTGCACAGCTTGCTGCTTGAGCGCAGGCCGACGCCACCGCGTCCACCGCGCCGGCATGACGGCAAGGTCGCCGTGGCCAAGAGCAACCAGCGC
TGGTGCTCCGATGGCTTCGAGTTCCGCTGCGACAACGGCGAGCCGCTGCGGGTGACGTTCGCGCTGGATTGCTGCGACCGCGAGGCGATGAGCTGGGTGG
CGACGACCGGCGGCTACAGCGGCGACGTAGTACGCGACGTGATGCTGGCGGCCGTCGAGCAGCGCTTCGGCAATGTTCCGAAAGCGCCAGCGGAAATTGA
ATGGCTGACGGACAACGGCTCAGGCTACATTGCTGGGAAGACTCGCGAGTTCGCCACGGACATTGGCTTGAAGCCATTGACGACACCGGTTTGCAGCCCG
CAGAGCAACGGCATGGCCGAGAGCTTCGTGAAGACGATGAAGCGCGACTACGTCGCCTTCATGCCCAAGCCTGACGCGGCCACAGCGACGCGCAACCTCG
CCATCGCGTTCGAGCATTACAACGAGCAGCATCCCCATAGCGCACTGAAATATCGCTCGCCACGCGAGTTCAGGCGCAGGACGGAATCATCAACTCAAGT
GTGAGGCGGTGTCCGGAGATGCGGGGGCAAATCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
369 bp122 aa91459+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MMEVLTGPERRRRWSVEEKLAMVRESFEPGKTVSMVARQHGVNPNQVFQWRKLYQDGSLSAVTAGEEVVPASELAEALKQVRELQRMLGKKTMENEILRE
AVEYGRAKKWIARSPSLPGDDQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
966 bp393 aa3391304+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AAAHARQEDDGERDSSRSRGIRSGKKVDSALAIVAGGRPVKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWGR
LRRERERQCEAPINAKRVYRVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVRD
VMLAAVEQRFGNVPKAPAEIEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATATRNLAIAFEHYNEQHPH
SALKYRSPREFRRRTESSTQV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1214 bp404 aa911304+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMEVLTGPERRRRWSVEEKLAMVRESFEPGKTVSMVARQHGVNPNQVFQWRKLYQDGSLSAVTAGEEVVPASELAEALKQVRELQRMLGKKTMENEILRE
AVEYGRAKKVDSALAIVAGGRPVKLVCEVLGVARSNVAAKRARPGDWRDGRTARQTDDASLVDEIRRTIADLPSYGYRRVWGRLRRERERQCEAPINAKR
VYRVMRVHSLLLERRPTPPRPPRRHDGKVAVAKSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWVATTGGYSGDVVRDVMLAAVEQRFGNVPKAP
AEIEWLTDNGSGYIAGKTREFATDIGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAFMPKPDAATATRNLAIAFEHYNEQHPHSALKYRSPREFRRRTES
STQV

 

Blast result :
Comments
There are 5 copies of ISRso10 on Ralstonia solanacearum genome (3 on chromosome and 2 on megaplasmid). One copie is disrupted by ISRso6.
References
1] Salanoubat,M., Genin,S., Artiguenave,F., Gouzy,J., Mangenot,S.,Arlat,M., Billault,A., Brottier,P., Camus,J.C., Cattolico,L., Chandler,M., Choisne,N., Claudel-Renard,C., Cunnac,S., Demange,N., Gaspin,C., Lavie,M., Moisan,A., Robert,C., Saurin,W., Schiex,T., Siguier,P., Thebault,P., Whalen,M., Wincker,P., Levy,M., Weissenbach,J. and Boucher,C.A. (2002) Nature 415 (6871), 497-502