ISRo1
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U70364 | ND | Rhodococcus opacus | Rhodococcus opacus MR11 plasmid pHG201 |
DNA section
IS Length : 1474 bp
Ends
IR Length : 15/23
IRL : GGCTAAGCCCCCTGGTGTGGTGGGAATTCGGTGAGGACGGCGTAGGCCCT
IRR : GATAGCGTCCCTTGGAGTGGTGGACTTCGGATCGGTGAACGAATCGGTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCCGTAAGG | CCGCTCCA | AGTTGTGGTG | 8 |
DNA sequence
GGCTAAGCCCCCTGGTGTGGTGGGAATTCGGTGAGGACGGCGTAGGCCCTGGTGAGGGTGTGTCGTTGCCCGGATATGGGTGGGCCATCGCGTAGTGATG
AACGAGTTACCTGCGACAGTGACTCGAATCAAGGAGCTACGCGATGACCCACGACCATTCTGCCCTGATCGCCCAGTTGGATGGGTTGAAGGGTGCTGAT
TCGGCGAGTGTGTTCGCCGAGTTGATCCGTGCCGGCATGCAGGCGTTGATCGACGCCGAGGCCACCGAGGCCCTTGGCGCCGGCCGGTACGAGCGTGCAG
AGGGCCGCACGACGTACCGCAACGGCAGTCGTGCCAAGACTGTGGCCACTACCAGCGGGGATGTGGAGCTCAAGATCCCGAAGTTGCGGTCGGGGTCGTT
CTTCCCGTCTCTGCTCGAGCGTCGACGCCGGATCGATCGGGCGTTGCATGCGGTGATCATGGAGGCCTACGTCCACGGTGTGTCGACCCGAAATGTCGAT
GACCTGGTCGGTGCGCTCGGCGTCGGCTCGGGTATCTCGAAGTCGGAGGTGTCACGGATCTGTGGGCAGCTCGATACCGAGATCGGCCGGTTCCGGGACC
GCACGTTGACGCACACCACGTTCCCCTATGTGTTCCTCGACGCCACGTACTGCAAGGTGCGGGTGGGGGCGCATGTCGTTTCTCATGCGTTGGTGGTGGC
GACCGGGGTATCAATGGATGGCACCCGCGAGGTTCTCGGGACTGCGGTCGGTGATAGTGAGTCGTTCGACTTCTGGCGGGAGTTCCTGGCGGGGTTGCGT
GCGCGTGGGCTCTGCGGGGTGCATCTGGTGATCTCTGATGCGCATGCCGGGTTGAAAGCGGCGGTGGCGCAGCAGTTCACGGGTTCGTCGTGGCAGCGGT
GTCGGGTGCATTTCATGCGGAATCTTCAGTCCGCGGTGCATTCGAAGCATGCGCCTGCGGTGATGGCGGCGGTGAAGACGGTGTTCGCGCACACCGACCC
CGACGAGGTGGCCGCACAGTGGGATCAGGTGACCGACACGCTCGCTGGATCATTTCCGAAGGTGTCATCGATGATGCGGGAGGCGAAGTGTGATGTGTTG
GCTTTCAGCGGTTTTCCGAGGTCGCATTGGCAGAAGATCTGGTCGAACAATCCGATCGAGCGGTTGAACAAGGAGATCAAACGCCGGGCCGATGTTGTGG
AGATCTTTCCGAATCCGGCGTCGTTCCTGCGGTTGGCGACGGCGGTGGTGATCGAGGCGCACGACGAGTGGCAGGTTACCCGCCGTTACGTCTCCGATGT
CAGTATGGACGAGCTCAGAACGGTCATCGTGGCAAAGGAACGCGCCGCCGCGCAACATGCCACAGCCGAATCGTTGACAGCGGAACTCGAAACCGCTTAA
AGTACACCATGACTCGTTGATCACGAACCGATTCGTTCACCGATCCGAAGTCCACCACTCCAAGGGACGCTATC
AACGAGTTACCTGCGACAGTGACTCGAATCAAGGAGCTACGCGATGACCCACGACCATTCTGCCCTGATCGCCCAGTTGGATGGGTTGAAGGGTGCTGAT
TCGGCGAGTGTGTTCGCCGAGTTGATCCGTGCCGGCATGCAGGCGTTGATCGACGCCGAGGCCACCGAGGCCCTTGGCGCCGGCCGGTACGAGCGTGCAG
AGGGCCGCACGACGTACCGCAACGGCAGTCGTGCCAAGACTGTGGCCACTACCAGCGGGGATGTGGAGCTCAAGATCCCGAAGTTGCGGTCGGGGTCGTT
CTTCCCGTCTCTGCTCGAGCGTCGACGCCGGATCGATCGGGCGTTGCATGCGGTGATCATGGAGGCCTACGTCCACGGTGTGTCGACCCGAAATGTCGAT
GACCTGGTCGGTGCGCTCGGCGTCGGCTCGGGTATCTCGAAGTCGGAGGTGTCACGGATCTGTGGGCAGCTCGATACCGAGATCGGCCGGTTCCGGGACC
GCACGTTGACGCACACCACGTTCCCCTATGTGTTCCTCGACGCCACGTACTGCAAGGTGCGGGTGGGGGCGCATGTCGTTTCTCATGCGTTGGTGGTGGC
GACCGGGGTATCAATGGATGGCACCCGCGAGGTTCTCGGGACTGCGGTCGGTGATAGTGAGTCGTTCGACTTCTGGCGGGAGTTCCTGGCGGGGTTGCGT
GCGCGTGGGCTCTGCGGGGTGCATCTGGTGATCTCTGATGCGCATGCCGGGTTGAAAGCGGCGGTGGCGCAGCAGTTCACGGGTTCGTCGTGGCAGCGGT
GTCGGGTGCATTTCATGCGGAATCTTCAGTCCGCGGTGCATTCGAAGCATGCGCCTGCGGTGATGGCGGCGGTGAAGACGGTGTTCGCGCACACCGACCC
CGACGAGGTGGCCGCACAGTGGGATCAGGTGACCGACACGCTCGCTGGATCATTTCCGAAGGTGTCATCGATGATGCGGGAGGCGAAGTGTGATGTGTTG
GCTTTCAGCGGTTTTCCGAGGTCGCATTGGCAGAAGATCTGGTCGAACAATCCGATCGAGCGGTTGAACAAGGAGATCAAACGCCGGGCCGATGTTGTGG
AGATCTTTCCGAATCCGGCGTCGTTCCTGCGGTTGGCGACGGCGGTGGTGATCGAGGCGCACGACGAGTGGCAGGTTACCCGCCGTTACGTCTCCGATGT
CAGTATGGACGAGCTCAGAACGGTCATCGTGGCAAAGGAACGCGCCGCCGCGCAACATGCCACAGCCGAATCGTTGACAGCGGAACTCGAAACCGCTTAA
AGTACACCATGACTCGTTGATCACGAACCGATTCGTTCACCGATCCGAAGTCCACCACTCCAAGGGACGCTATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1257 bp | 418 aa | 144 | 1400 | + | No |
Chemistry : DDE
ORF sequence :
MTHDHSALIAQLDGLKGADSASVFAELIRAGMQALIDAEATEALGAGRYERAEGRTTYRNGSRAKTVATTSGDVELKIPKLRSGSFFPSLLERRRRIDRA
LHAVIMEAYVHGVSTRNVDDLVGALGVGSGISKSEVSRICGQLDTEIGRFRDRTLTHTTFPYVFLDATYCKVRVGAHVVSHALVVATGVSMDGTREVLGT
AVGDSESFDFWREFLAGLRARGLCGVHLVISDAHAGLKAAVAQQFTGSSWQRCRVHFMRNLQSAVHSKHAPAVMAAVKTVFAHTDPDEVAAQWDQVTDTL
AGSFPKVSSMMREAKCDVLAFSGFPRSHWQKIWSNNPIERLNKEIKRRADVVEIFPNPASFLRLATAVVIEAHDEWQVTRRYVSDVSMDELRTVIVAKER
AAAQHATAESLTAELETA
LHAVIMEAYVHGVSTRNVDDLVGALGVGSGISKSEVSRICGQLDTEIGRFRDRTLTHTTFPYVFLDATYCKVRVGAHVVSHALVVATGVSMDGTREVLGT
AVGDSESFDFWREFLAGLRARGLCGVHLVISDAHAGLKAAVAQQFTGSSWQRCRVHFMRNLQSAVHSKHAPAVMAAVKTVFAHTDPDEVAAQWDQVTDTL
AGSFPKVSSMMREAKCDVLAFSGFPRSHWQKIWSNNPIERLNKEIKRRADVVEIFPNPASFLRLATAVVIEAHDEWQVTRRYVSDVSMDELRTVIVAKER
AAAQHATAESLTAELETA
Blast result :
Comments
ISRo1 is located upstream of hoxF, hoxU, hoxY, hoxH and HoxW, a gene cluster encoding the NAD-reducing hydrogenase in the linear plasmid pHG201 from Rhodococcus opacus (Grzeszik et al., 1997). ISRo1 putative transposase is 59 % identical to that of IS1311 from Mycobacterium avium.
References
1] Grzeszik, C., Lubbers, M., Reh, M., and Schlegel, H.G. (1997) Microbiology 143, 1271-1286.