ISRm4-1
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| Y13432 | ND | Rhizobium meliloti | Rhizobium meliloti plasmid pRmNT40 |
DNA section
IS Length : 937 bp
Ends
IR Length : 15/21
IRL : GAGGCTGCCTCAAAAAGAATTGAGTGATTTCAGCCAGTTGTGATTCCGTC
IRR : GAGTCGGACTCGAAAAGGTTTCAACGATATCCATATCTTGCAAGGCGTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TTA | NNNNNNNNNN | 3 |
DNA sequence
GAGGCTGCCTCAAAAAGAATTGAGTGATTTCAGCCAGTTGTGATTCCGTCGGATTTGCAAAGATTTCGATGGAGATCACGATGGCCTGGACTGAAACCAC
CCGGCGCAATTATGTCCGGCGGACGAGCGGATATGCAAGCGATGTCACGGATCGCGAATGGGATTTTGTTGCCCCGTTCATGCCTGCGCCACGGCGTCTG
GGTCGTCCGCGCAAGACTGATTTGCGCGATGTTTTAAACGCCCTTCTCTATATCGCTTCGACAGGCTGCCAGTGGCGGATGCTGCCGAAGGACTTTCCGC
CCTGTTCGACGGTGCAGCGATATTTCTATGAATGGCGGGCAATGGGTCTTTGGCCACGGATCAACCATCACCTCGTCATGGAGGAGGCGCGGGAATTGGA
GGGGAAGGAAGCCTCGCCGACAGCGGGCGCGATCGACAGCCAAAGCGTTAAAACGACGGAAAGCGGCGGTATCCGTGGCTTTGATGCCGGCAAGAAGATT
AAGGGCCGCAAGCGCCACATCATTGTCGACACGCTCGGGCTGATGGTCGGCCTCATGGTGCACAGCGCCGATATTCAGGATCGCGACGGCGCTCCCGATC
TCCTGAAATCCATCAAAAACCGGTGGCCGTGGTTGCTTCATGTCTTCGCTGATGGCGGCTATGCGGGCGACAAGCTCAAAAAGCGGCTGCAGAAAATCGG
GAAATGGACACTCGAAATCATCAAACGTTCCGACAAGGCCAAGGGTTTTGAAATCCTGCCGCGCCGCTGGGTCGTCGAGCGGACCTCCGCCTGGCTGGGT
CGATGCCGCAGACTGGCCAAGGACTTCGAAAAATCCGTCGCTTCAGCAGAAGCGTGGATCACCATCGCCCACATCCGCATGCTCACCAGACGCCTTGCAA
GATATGGATATCGTTGAAACCTTTTCGAGTCCGACTC
CCGGCGCAATTATGTCCGGCGGACGAGCGGATATGCAAGCGATGTCACGGATCGCGAATGGGATTTTGTTGCCCCGTTCATGCCTGCGCCACGGCGTCTG
GGTCGTCCGCGCAAGACTGATTTGCGCGATGTTTTAAACGCCCTTCTCTATATCGCTTCGACAGGCTGCCAGTGGCGGATGCTGCCGAAGGACTTTCCGC
CCTGTTCGACGGTGCAGCGATATTTCTATGAATGGCGGGCAATGGGTCTTTGGCCACGGATCAACCATCACCTCGTCATGGAGGAGGCGCGGGAATTGGA
GGGGAAGGAAGCCTCGCCGACAGCGGGCGCGATCGACAGCCAAAGCGTTAAAACGACGGAAAGCGGCGGTATCCGTGGCTTTGATGCCGGCAAGAAGATT
AAGGGCCGCAAGCGCCACATCATTGTCGACACGCTCGGGCTGATGGTCGGCCTCATGGTGCACAGCGCCGATATTCAGGATCGCGACGGCGCTCCCGATC
TCCTGAAATCCATCAAAAACCGGTGGCCGTGGTTGCTTCATGTCTTCGCTGATGGCGGCTATGCGGGCGACAAGCTCAAAAAGCGGCTGCAGAAAATCGG
GAAATGGACACTCGAAATCATCAAACGTTCCGACAAGGCCAAGGGTTTTGAAATCCTGCCGCGCCGCTGGGTCGTCGAGCGGACCTCCGCCTGGCTGGGT
CGATGCCGCAGACTGGCCAAGGACTTCGAAAAATCCGTCGCTTCAGCAGAAGCGTGGATCACCATCGCCCACATCCGCATGCTCACCAGACGCCTTGCAA
GATATGGATATCGTTGAAACCTTTTCGAGTCCGACTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 585 bp | 245 aa | 181 | 765 | + | No |
Chemistry : DDE
ORF sequence :
MPAPRRLGRPRKTDLRDVLNALLYIASTGCQWRMLPKDFPPCSTVQRYFYEWRAMGLWPRINHHLVMEEARELEGKEASPTAGAIDSQSVKTTESGGIRG
FDAGKKIKGRKRHIIVDTLGLMVGLMVHSADIQDRDGAPDLLKSIKNRWPWLLHVFADGGYAGDKLKKRLQKIGKWTLEIIKRSDKAKGFEILPRRWVVE
RTSAWLGRCRRLAKDFEKSVASAEAWITIAHIRMLTRRLARYGYR
FDAGKKIKGRKRHIIVDTLGLMVGLMVHSADIQDRDGAPDLLKSIKNRWPWLLHVFADGGYAGDKLKKRLQKIGKWTLEIIKRSDKAKGFEILPRRWVVE
RTSAWLGRCRRLAKDFEKSVASAEAWITIAHIRMLTRRLARYGYR
Blast result :
Comments
ISRm4-1 carries an insertion of ISRm9 at coordonates 153. This has been excised in silico together with one of the two 7bp DRs to generate ISRm4-1. 83% aa homologous with IS1031A and 80% with ISRm4.
References
1] Zekri,S., Soto,M.J. and Toro,N.(1998) Gene 207 (1), 93-96