ISfinder Mirror Site

ISRm2

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HE995405	Y	Sinorhizobium meliloti	Rhizobium trifolii 24K/56 Sinorhizobium meliloti Rm41 Sinorhizobium meliloti Rm41 plasmid pRM41A Rhizobium leguminosarum JB897 Rhizobium meliloti 41 plasmid pRme41c Rhizobium meliloti 1021

DNA section

IS Length : 2593 bp

Ends

IR Length : 24/25

IRL : GTAAGCGCTCATTTCCATGCGCCTTTTCTCGTTGATGCCTCTGCCGCATG
IRR : GTAAGCGCTCATTTCCATGCGCGTTGACGCGGCCCGTTTGACCGGGGTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCCACGTCCT	GATGAACG	TGTGGTCTTC	8
TAAGCCCGTA	AATGGACA	ATCCGATTTA	8
GTGTTGTGCG	AGGCAAAC	GGTGACGTCG	8
GGGAAGGTTC	GCAAAAGT	GATCTCTTCA	8
AAGCAGGATA	CGTTTAAT	CGAGTACGAC	8

DNA sequence

GTAAGCGCTCATTTCCATGCGCCTTTTCTCGTTGATGCCTCTGCCGCATGAAGTGTCCACTTAAAATAGGTGGACACCAAATATGAGTGACGAAGAGCAG
AAACTGCGTGTGCGGCATGTGGGGCGCGATGGTCGACGCCGCTACGATCCAGGATCAAAGGCGCGGCTTGTTGCGGCCTGCCTTGAGCCTGGTGTGTCGA
TATCCGGCCTTGCGCTTACGCATGGGATCAATGCCAACCTACTGCGCAAGTGGGTCAAGGAGGCCAGGGAGTCTGGCCCCCTGGCGGTATCTCCACGATC
GGCGTTTATCCCGGTCGTGGCAGCAGACGGCAGCCTGCCTGTCGGGACCTGCTCGCTGGATATGTCGGTCACGTGCGATGAAGAACAACGAGCATCACTG
GAGAAGACGGTTCATCCGTCAGGTTTATCCAAGATCAGCGCGTTGCTGCCAAACGGCGTAAAGCTTTCGCTGGAATGCAGGGATGTGGATGCGTTGACGG
CTGTCATTGGAGCATTGGGCCATGTTCAGACTGGGCGCTGATCTCAAGGTCTACCTGCACCGCGAACCGATCGACTTCCGCGCCGGCATCAACAGCCTTG
CGGTCCTGGTGCAGGAGACGATGGAGCTCGACCCGTTTGCGCCTGCGGTCTTTGCCTTTTGCAATCGCCGCCGTGACCGGATGAAGCTCCTGTTCTTCGA
TCGGTCCGGTTTTGTGATGGTCCTGAAGCGATTGACCGAAGATCGGTTCAGATGGCCACGCCGGGAGACGGCGGTGGTGACGCTTTCGACTGAGCAGCTT
CACTGGATTCTCGACGGCATCGATATCGACGCCATGGTGCGCCACCCTGTGCGGCAATATCAGATCGCCGGCTGACGGCTCTGAACTTCTGCGGTTGACG
CGGTGGTGCGGTTCAGATTCAAAACTACGATGACTCGATCCGGCGAACCAAGCGTTGCAGAACTGATGGCGCAGTTGGCGGCAAATGCTGCCGAAATCGC
CGCGCTGAAAGCCGAGAAAGAAGCGCTCAGCGCCCGCGTGGTCAAGCTCGAGGAAGAGTTGGAGCTGGCGCGGCTGCATCGTTTTGCGCCGAAGAGCGAA
AAGCATGTGGACCGGCTCTTCGATGAAGCCGAGCAGGTTGCCAATGAAGACGAGGCCAGCGGAGAAGATGGCGAGGTCGTCGACCTGCCGGAAACCGGTT
TTGCGACGATCGAGAAACCGGAGGGCAAGAAGCGTGGCCGCAAGCCCCTGCCGGAACACCTGCCGCGCGAGCGTGTCGAGTATGACCTTGCCGACGACCA
GAAGGCCTGCCCTTGCTGCCGTCATCAGATGCATCGCATGGGTGAGGCCGTCACCGAGCAGCTCCATATCGAAGTGAGGGCCAAGGTCCTGCAGAATGTG
CGGTTCAAGTACGCCTGCCGCCATTGCGACCGGACCGGCATCAACACGCCTGTCGTTCTCGCGCCAATGCCCGCGCAGCCCTTGCCGGGCAGCATCGCCA
CGGCCTCGACGCTGGCATTTGCACTGGTCCACAAATACGTCGACGGCACGCCGCTCTACCGTCTGGCCCAGGCCTTCGAGCGCGCCGGCGTTCCCGTCAG
CCGCGGTGCTTTGGGTCACTGGGTGATCGGCTCGAGCGAGAGGCATCTCTCTCGCATCTATGACGCGCTGAAGCTCCGGCTTCGTTCACAACCGCTGATC
CATGGTGACGAGACAACGGTCCAGGTGCTGAAGGAAAAGGACAGGGAGGCCACCAGCACATCCTTCATGTGGGCATACAGGAGCGGTGAAGACAGCGGCG
AGCCGATCGTGCTGCTCGATTATCAACCCGGCCGCGGCCAGATTTATCCGCAGGCCTTCCTCGGCGACTACCGCGGCATATTGATGAGCGATGGTTACAC
CGCCTGGCGCACTCTGGCAGGAGCGACCCATATTGGGTGTATGGCCCATTCGAGGCGTCGCTTCATCGATGCTCTGAAGACAAGGAAGAAAGGCGGCGGA
CCGCCGGAGCAGGCGCTGAAGTTCTTCGAGCAGCTCTACCGGATTGAAAGCCAAGCCAAGAACGAAAAGCCGGAAGTTGGCGAAACGCAAGCCGACTGCA
TCCGCCGTTTCCGCCAGCAACACAGCGTCCCCATCCTGATCGCTCTCAAGGCATGGCTCGAGGACATCGCGCCGAAGGTCTTGCCCGATAGCAAGATCGG
CGACGCCGTATCCTACACCCTGAACCAGTGGGAATACCTGACGCGCTACACCGAAGACGGCAGGATGTCGATCGACAACAACCTTCTCGAACGCGATATC
AGGATTTTTGCCACTGGAAGAAAATCCTGGCTGTTCAGCGATACCGTGGACGGAGCCAGGGCCAGTGCTGTCGTCTACAGCATCATGCTGACCTGTCGCG
CCTGCGGCGTCGAGCCCTTGGCCTACTTGCGCCACGTGCTTAGTGAATTGCCACAGCGCGCAACAGATACTGACATCACCGATCTCTTGCCCTTCAACTT
CACCAAAGCAGCCGCTGCCTGATCCAATGCAGCCGTCGCACAGCGACCCCGGTCAAACGGGCCGCGTCAACGCGCATGGAAATGAGCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
459 bp	152 aa	83	541	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSDEEQKLRVRHVGRDGRRRYDPGSKARLVAACLEPGVSISGLALTHGINANLLRKWVKEARESGPLAVSPRSAFIPVVAADGSLPVGTCSLDMSVTCDE
EQRASLEKTVHPSGLSKISALLPNGVKLSLECRDVDALTAVIGALGHVQTGR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	522	875	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MFRLGADLKVYLHREPIDFRAGINSLAVLVQETMELDPFAPAVFAFCNRRRDRMKLLFFDRSGFVMVLKRLTEDRFRWPRRETAVVTLSTEQLHWILDGI
DIDAMVRHPVRQYQIAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1557 bp	530 aa	966	2522	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRSGEPSVAELMAQLAANAAEIAALKAEKEALSARVVKLEEELELARLHRFAPKSEKHVDRLFDEAEQVANEDEASGEDGEVVDLPETGFATIEKPEGK
KRGRKPLPEHLPRERVEYDLADDQKACPCCRHQMHRMGEAVTEQLHIEVRAKVLQNVRFKYACRHCDRTGINTPVVLAPMPAQPLPGSIATASTLAFALV
HKYVDGTPLYRLAQAFERAGVPVSRGALGHWVIGSSERHLSRIYDALKLRLRSQPLIHGDETTVQVLKEKDREATSTSFMWAYRSGEDSGEPIVLLDYQP
GRGQIYPQAFLGDYRGILMSDGYTAWRTLAGATHIGCMAHSRRRFIDALKTRKKGGGPPEQALKFFEQLYRIESQAKNEKPEVGETQADCIRRFRQQHSV
PILIALKAWLEDIAPKVLPDSKIGDAVSYTLNQWEYLTRYTEDGRMSIDNNLLERDIRIFATGRKSWLFSDTVDGARASAVVYSIMLTCRACGVEPLAYL
RHVLSELPQRATDTDITDLLPFNFTKAAAA

Blast result :

Comments

The relationship between ISRm2 and the IS66 family was made by Ponsonnet et al. (1994).
The four Accession numbers (M21471, M21472, M15786, M15785) correspond to right and left ends.
ISRm2 is 54%(orfA), 87%(orfB) and 85%(orfC) aa similar to IS692.

References

1] Dusha, I., Kovalenko, S., Banfalvi, Z., and Kondorosi, A. (1987) J. Bacteriol. 169, 1403-1409.
2] Ponsonnet, C., Normand, P., Pilate, G., Chapelon, C., and Nesme, X. (1994) submitted.
3] Weidner,S., Baumgarth,B., Goettfert,M., Jaenicke,S., Puehler,A., Schneiker-Bekel,S., Serrania,J., Szczepanowski,R. and Becker,A. (2012) Direct submission GenBank