ISRle39B
- Family IS6
- Group
Isoform ISRle39ASynonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X99520 | ND | Rhizobium leguminosarum | Rhizobium leguminosarum VF39SM |
DNA section
IS Length : 890 bp
Ends
IR Length : 17/22
IRL : GGGCGTGTCGCAAAGTTTTCTGTTAACGCGGATTTGAGATTTGGATGGTG
IRR : GGGGCTGTCGCAAACATTTTTGCGGCTCGCAATTATCCCATGAACGGCGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGGCGTGTCGCAAAGTTTTCTGTTAACGCGGATTTGAGATTTGGATGGTGAAACGGCGCTCCGATTTGTTGGAGCGCGGTTTCATGATTCTGAATGCGAT
TGCCGAAAAACTTAAACGCCAGTCGAAGGACGATTTCAAAGGCCGGCAATTTGAAGCGTGGCTGATCATTCAGGCGGTTTCCTGGTATTTGCGCTATCCG
TTGAGCTATCGCGATCTCGAGGAAATGTTCAAGGAACGCGGCTTCGAGGTGGATCACAGCAGCGTCAATCGTTGGGTGTTGGCCTATGCGCCGATGATTG
AGAAGCGGTTGCGCCAGTTCCGGAGACCGCATTGTGGTTCGATCCGGGTCGATGAAACATACGTGAAAATTCGGGGTCAATGGCGCTATCTGTACCGGGC
TATCGATAAACACGGCAATCCGGTGGATTTCCTGCTCACCGCAAAGCGTGACCTCGACGCTGCCAAGCGTTTCTTTCGCAAGATGCTGAAGGATGAGCCT
TTGCTTTCACCCGGCAAAATCGGCACAGATGGTGCCGATACCTTTCCTTCAACGATCAAAACCTCGGTCGAAGAGGGCTGCTCCATCCTGATCCCGTGCA
TTATGTCACCAAGCACCTGCAGCAAGGCATTGAAAGCGACCATTTTCGGGTGAAAAAGAACATGCCGAAAATCGGTGGGTTTCGATCATTCAATTCAGCC
CGGCGAACGATCGCAGGTTTCGAGGCGATACTGTGGTTGAAGAAAGGCTTCGGCTTTTCTGGCGGCTGGACCGTCCACGATCAGAATGATCTGCTTGCGC
GCCTCTTCGGACTTCAAAAAGTTAACAAAGCATGAAAATGACGCCGTTCATGGGATAATTGCGAGCCGCAAAAATGTTTGCGACAGCCCC
TGCCGAAAAACTTAAACGCCAGTCGAAGGACGATTTCAAAGGCCGGCAATTTGAAGCGTGGCTGATCATTCAGGCGGTTTCCTGGTATTTGCGCTATCCG
TTGAGCTATCGCGATCTCGAGGAAATGTTCAAGGAACGCGGCTTCGAGGTGGATCACAGCAGCGTCAATCGTTGGGTGTTGGCCTATGCGCCGATGATTG
AGAAGCGGTTGCGCCAGTTCCGGAGACCGCATTGTGGTTCGATCCGGGTCGATGAAACATACGTGAAAATTCGGGGTCAATGGCGCTATCTGTACCGGGC
TATCGATAAACACGGCAATCCGGTGGATTTCCTGCTCACCGCAAAGCGTGACCTCGACGCTGCCAAGCGTTTCTTTCGCAAGATGCTGAAGGATGAGCCT
TTGCTTTCACCCGGCAAAATCGGCACAGATGGTGCCGATACCTTTCCTTCAACGATCAAAACCTCGGTCGAAGAGGGCTGCTCCATCCTGATCCCGTGCA
TTATGTCACCAAGCACCTGCAGCAAGGCATTGAAAGCGACCATTTTCGGGTGAAAAAGAACATGCCGAAAATCGGTGGGTTTCGATCATTCAATTCAGCC
CGGCGAACGATCGCAGGTTTCGAGGCGATACTGTGGTTGAAGAAAGGCTTCGGCTTTTCTGGCGGCTGGACCGTCCACGATCAGAATGATCTGCTTGCGC
GCCTCTTCGGACTTCAAAAAGTTAACAAAGCATGAAAATGACGCCGTTCATGGGATAATTGCGAGCCGCAAAAATGTTTGCGACAGCCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 752 bp | 250 aa | 84 | 835 | + | Yes |
Chemistry : DDE
ORF sequence :
MILNAIAEKLKRQSKDDFKGRQFEAWLIIQAVSWYLRYPLSYRDLEEMFKERGFEVDHSSVNRWVLAYAPMIEKRLRQFRRPHCGSIRVDETYVKIRGQW
RYLYRAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPGKIGTDGADTFPSTIKTSVEEGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGF
RSFNSARRTIAGFEAILWLKKGFGFSGGWTVHDQNDLLARLFGLQKVNKA
RYLYRAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPGKIGTDGADTFPSTIKTSVEEGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGF
RSFNSARRTIAGFEAILWLKKGFGFSGGWTVHDQNDLLARLFGLQKVNKA
Blast result :
Comments
Two slightly different copies of ISRle39 (6 bp different between A and B) flank a new plasmid-encoded restriction-modification system (homologous to the PstI system) in Rhizobium leguminosarum VF39SM (Rochepeau et al., 1996), in a 4.8-kb transposon-like structure. ISRle39B putative transposase is a fusion between two ORFs (Sequencing error ?): 84-578 and 578-835. ISRle39A and ISRle39B putative transposases are 98 % identical.
References
1] Rochepeau, P., Selinger, L.B., and Hynes, M.F. (1996) "Transposon-like structure of a new plasmid encoded restriction-modification system in Rhizobium leguminosarum VF39SM" EMBL Data Library, Rel. 49.