ISPsp1

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
M57500 ND Pseudomonas sp.
Pseudomonas sp. EST1001 plasmid pEST1226
DNA section
IS Length : 1188 bp

Ends


IR Length : 9/12

IRL : GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACC
IRR : GGATGGTCTGAAGTAGCCATGTATTTCCCCGGCATACCACCTAGCGCAAC

Insertion site


Left flankDirect repeatRight flankDR Length
AGCAGTGTTCTTAAGGCTCAAGCC4

DNA sequence

GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACCCACCGAGTTTCCCGATGAAGCAGATGACCTTCGCCGACGCCGAGTACGCT
GGCAAGCGCAAGCAAACCCGCAAGGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGCTTGATTGCTTTGATCGAGCCACATTATCCGA
AAGGTGAAGGTGGCCGTCCGGCCTACCCGTTGATGGCGATGCTGCGTGTGCATCTGCTGCAGAACTGGTTCGGCTACAGCGATCCAGCGATGGAGGAAGC
GCTGTACGAAACCACGATCCTGCGCCAGTTTGCCGGGCTGAACCTGGAGCGCATCCCCGACGAAACCACCATTCTCAACTTCCGCCGCTTGCTGGAGAAA
CACGAGCTGGCGGCCGGCATCCTCGCTGTCATCAATGGCTATCTGGGCGACCGCGGCCTGTCGCTGCGCCAGGGCACCATCGTCGATGCAACGCTGATCA
ATGCGCCCAGTTCGACCAAGAACAAGGACGGCAAGCGCGACCCGGAAATGCACCAGACCAAGAAGGGAAACCAGTATTATTTTGGCATGAAGGCCCACAT
CGGCGCCGATGACGAATCGGGTCTGGTGCACAGCGTAGTGGGCACGGCGGCCAATGTGGCGGATGTCACCCAGGTGGACAAATTGCTGCATGGCGACGAA
AACGTGGTCTGCGCCGATGCAGGCTACACCGGTGTCGAAAAGCGGCCCGAGCATGAAGAACGTGAAGTTATCTGGCAGGTGCGGCACGCCGCAGCACCTA
CAAAAAACTCGATAAGCGCAGCGTGCTGTACAAAGCCAAGCGCAAGATTGAAAAGGCCAAGGCTCAGGTGCGCGCCAAGGTCGAGCATCGTTCCGGGTAA
TCAAGCGCCAGTTCGGTTACACCAAGGTGCGCTTTCGCGGCTTGGCCAAAAACACGGCGCAACTGGTGACACTGTTCGCTCTGTCGAACCTGTGGATGGC
GCGCCGACATTTACTGACGAATGCAGGAGAGGTGCGCCTGTAATGCGGGAAATGGCCACCGGAGTGGGGTCCGGGTGGCGCAAACGTAAGAAATACTCGT
CGAATTGATTGTTTTTTGATCATTTGGCGAGTTTAAAAGTTGCGCTAGGTGGTATGCCGGGGAAATACATGGCTACTTCAGACCATCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
970 bp323 aa741043+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTFADAEYAGKRKQTRKELFLIEMDRVVPWKGLIALIEPHYPKGEGGRPAYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLNLERIPDETTI
LNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADDESGLVHSVVGTAANVADVTQ
VDKLLHGDENVVCADAGYTGVEKRPEHEEREVIWAGAARRSTYKKLDKRSVLYKAKRKIEKAKAQVRAKVEHRFRVIKRQFGYTKVRFRGLAKNTAQLVT
LFALSNLWMARRHLLTNAGEVRL

 

Blast result :
Comments
ISPsp1 is located 229 bp upstream of the pheB structural gene (catechol 1, 2-dioxygenase). There are most probably 2 or 3 sequencing errors in the second part of the ORF; the "new" ORF results from the fusion between (74-775), (775-891) and (891-1043). The limits of ISPsp1 (including the IR and DR) have been deduced by analogy with IS5. ISPsp1 is closely related to IS52 (60 % identity for their transposases).
References