ISPpu7
- Family IS21
- Group
Isoform Synonym(s) ISS12
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF292393 | ND | Pseudomonas putida | Pseudomonas putida S12 |
DNA section
IS Length : 2598 bp
Ends
IR Length : 13/14
IRL : TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCGCATCCGGACAGT
IRR : TGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGCGGATTCCACGCTGACTCGGACACCCATTCCACGCGCATCCGGACAGTGATTCCACGCTGATCCGGACACTCATTCCACGAGCATCCGGACACCGACT
CCACGGTCATTCCGGACACTTTTCTGGCAGGCAGCCACGCAGGATTTATTCACTACCATCGATCTCTTTTTCGAAGCAGAGAGGTCGTTGTGGAGCGTTT
ATCCATGCGTAAAATCCGAGAGGTGTTACGCCTCAAGTTCGACTGCGGCCTGTCCGTGCGCAAGATCGCCCGCAGCCTGGGCATTGGCCACAGCAGTGCC
GGTGATTACCTCTGCCGTTTTGCCGCCAGCGGCCTCACCTGGCCCTGTTCGTTGTCCGATGCCGAGTTGGAGCAGCAACTGTTCCCGCCGGCCCCGGCGG
TTGCCAGTGAGAAGCGGCCTTTACCCGATTGGGCATGGGTGCATGCCGAACTGCGCCGCCCCGGGGTGACCCTGGCGCTGCTCTGGCAGGAGTACCGCCT
GAGCCAGCCTCAGGGCTTTCAGTACAGCTGGTTCTGTGAGCACTACCGAGCCTGGCAGGGCAAGTTGGACGTGGTGATGCGTCAGGAGCACCGCGTCGGC
GAGAAGCTGTTCGTCGACTATGCCGGCCAGACGGTGCCGGTTATCGATCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCG
CGTCCAGCTACACCTTCGCCGAAGCCACCTGGTCGCAGCAGCTGCCGGACTGGCTAGGCTCCCATGCCCGCTGCTTCGCCTTCCTCGGCGGCGTGCCGGA
GATCGTGGTGCCGGACAACCTGCGCAGCGCGGTGAGCAAGAGTCACCGCTACGAGCCGGACATCAACCCCAGCTACCGCGATCTGGCCGAGCACTATGGC
GTGGCGGTGGTGCCGGCGCGGGCACGCAAACCGCGCGACAAGGCCAAGGCCGAAGTCGGCGTGCAGGTGGTCGAGCGTTGGATCCTCGCCGCACTGAGGA
ATCGGCAGTTCTTCTCCCTGGATGAACTCAACACGGCCATCGCCGGGCTGCTGGAGCGGCTCAACCAACGCCCGTTCAAGAAGCTGCCGGGCTCCCGGCA
GTCGGCCTTCGACAGCCTGGATCGTCCGGCGCTGCGCCCCCTGCCGGAGCAACCCTACGTCTACGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTAC
CACGTCGAGGTCGATGGGCATTACTACTCGGTGCCGTATCAACTGGTGAAGAAGCAGCTGGAGGTGCGCCTGACGGCGCGCACCGTCGAGTTTTTCCACG
CCAACCAGCGAGTGGCCAGCCACCTGCGCTCAATGCACAAGGGCAGGCACAGCACGCAGGCCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTG
GACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACCGCCGGCGTGATCCGGCACATCCTCGAACGGCGCATCCATCCGCAGCAGGGC
TACCGGGCCTGCCTGGGCATCCTGCGCCTGGGTAAAACCCACGGTGAGGCGCGTCTGGAGTTGGCCTGCCGTCGCGCCATCAGCCTCGGCACGTGCAGCT
ACAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAACCAACCTGCCGCTGCTGCCGGACGACCACGCCAACCTGCGCGG
ATCCGCCTACTACCACTGACCCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTGCAAACCCTGCGCCTGCACGGCATGCTCAAGGCGC
TGAATGAACAACTGAAAACCCCGGACATCGACAGCCTGAGCTTCGAAGAACGCCTCGGCCTGCTGGTCGACCGCGAGCTGACCGAACGCGATGACAAGCG
CCTGAGCAGCCGCCTGCGCCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTGGATAAGGCGCTGATCCTG
CAACTGAGCAGTGGTCAGTGGCTGCGCGACGGCCTCAACCTGATCATCGGCGGCCCCACCGGTGTCGGTAAAACCTGGCTGGCCTGCGCCCTGGCCCACC
AGGCCTGCCGGGAGGGCTACAGCGTGCGCTACCTGCGCCTGCCACGTTTGCTGGAAGAACTGGGTCTGGCCCATGGCGACGGCCGCTTCGCCAAGCTGAT
GAGCAGCTACGCCAAGACCGACCTGCTGATCCTCGACGACTGGGGCCTGGCCCCGTTCACCGGCGAGCAACGGCGCGACATGCTGGAGCTACTGGACGAC
CGTTACGGCCAGCGCTCGACCATCGTCACCAGCCAGATGCCGGTGGACAACTGGCACGAACTGATCGGCGATCCGACCCTGGCCGATGCCATCCTCGACC
GCCTGGTGCACAACGCTTATCGGATCAATCTGAAGGGTGAATCAATGCGCAAACGGACGCAGAAATTGACGACGCCAGCCAACCCGGACTAACAATGCCA
CCCCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGAGCGTGGAACCAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
CCACGGTCATTCCGGACACTTTTCTGGCAGGCAGCCACGCAGGATTTATTCACTACCATCGATCTCTTTTTCGAAGCAGAGAGGTCGTTGTGGAGCGTTT
ATCCATGCGTAAAATCCGAGAGGTGTTACGCCTCAAGTTCGACTGCGGCCTGTCCGTGCGCAAGATCGCCCGCAGCCTGGGCATTGGCCACAGCAGTGCC
GGTGATTACCTCTGCCGTTTTGCCGCCAGCGGCCTCACCTGGCCCTGTTCGTTGTCCGATGCCGAGTTGGAGCAGCAACTGTTCCCGCCGGCCCCGGCGG
TTGCCAGTGAGAAGCGGCCTTTACCCGATTGGGCATGGGTGCATGCCGAACTGCGCCGCCCCGGGGTGACCCTGGCGCTGCTCTGGCAGGAGTACCGCCT
GAGCCAGCCTCAGGGCTTTCAGTACAGCTGGTTCTGTGAGCACTACCGAGCCTGGCAGGGCAAGTTGGACGTGGTGATGCGTCAGGAGCACCGCGTCGGC
GAGAAGCTGTTCGTCGACTATGCCGGCCAGACGGTGCCGGTTATCGATCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCG
CGTCCAGCTACACCTTCGCCGAAGCCACCTGGTCGCAGCAGCTGCCGGACTGGCTAGGCTCCCATGCCCGCTGCTTCGCCTTCCTCGGCGGCGTGCCGGA
GATCGTGGTGCCGGACAACCTGCGCAGCGCGGTGAGCAAGAGTCACCGCTACGAGCCGGACATCAACCCCAGCTACCGCGATCTGGCCGAGCACTATGGC
GTGGCGGTGGTGCCGGCGCGGGCACGCAAACCGCGCGACAAGGCCAAGGCCGAAGTCGGCGTGCAGGTGGTCGAGCGTTGGATCCTCGCCGCACTGAGGA
ATCGGCAGTTCTTCTCCCTGGATGAACTCAACACGGCCATCGCCGGGCTGCTGGAGCGGCTCAACCAACGCCCGTTCAAGAAGCTGCCGGGCTCCCGGCA
GTCGGCCTTCGACAGCCTGGATCGTCCGGCGCTGCGCCCCCTGCCGGAGCAACCCTACGTCTACGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTAC
CACGTCGAGGTCGATGGGCATTACTACTCGGTGCCGTATCAACTGGTGAAGAAGCAGCTGGAGGTGCGCCTGACGGCGCGCACCGTCGAGTTTTTCCACG
CCAACCAGCGAGTGGCCAGCCACCTGCGCTCAATGCACAAGGGCAGGCACAGCACGCAGGCCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTG
GACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACCGCCGGCGTGATCCGGCACATCCTCGAACGGCGCATCCATCCGCAGCAGGGC
TACCGGGCCTGCCTGGGCATCCTGCGCCTGGGTAAAACCCACGGTGAGGCGCGTCTGGAGTTGGCCTGCCGTCGCGCCATCAGCCTCGGCACGTGCAGCT
ACAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAACCAACCTGCCGCTGCTGCCGGACGACCACGCCAACCTGCGCGG
ATCCGCCTACTACCACTGACCCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTGCAAACCCTGCGCCTGCACGGCATGCTCAAGGCGC
TGAATGAACAACTGAAAACCCCGGACATCGACAGCCTGAGCTTCGAAGAACGCCTCGGCCTGCTGGTCGACCGCGAGCTGACCGAACGCGATGACAAGCG
CCTGAGCAGCCGCCTGCGCCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTGGATAAGGCGCTGATCCTG
CAACTGAGCAGTGGTCAGTGGCTGCGCGACGGCCTCAACCTGATCATCGGCGGCCCCACCGGTGTCGGTAAAACCTGGCTGGCCTGCGCCCTGGCCCACC
AGGCCTGCCGGGAGGGCTACAGCGTGCGCTACCTGCGCCTGCCACGTTTGCTGGAAGAACTGGGTCTGGCCCATGGCGACGGCCGCTTCGCCAAGCTGAT
GAGCAGCTACGCCAAGACCGACCTGCTGATCCTCGACGACTGGGGCCTGGCCCCGTTCACCGGCGAGCAACGGCGCGACATGCTGGAGCTACTGGACGAC
CGTTACGGCCAGCGCTCGACCATCGTCACCAGCCAGATGCCGGTGGACAACTGGCACGAACTGATCGGCGATCCGACCCTGGCCGATGCCATCCTCGACC
GCCTGGTGCACAACGCTTATCGGATCAATCTGAAGGGTGAATCAATGCGCAAACGGACGCAGAAATTGACGACGCCAGCCAACCCGGACTAACAATGCCA
CCCCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGAGCGTGGAACCAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1530 bp | 509 aa | 190 | 1719 | + | No |
Description :
ORF sequence :
MERLSMRKIREVLRLKFDCGLSVRKIARSLGIGHSSAGDYLCRFAASGLTWPCSLSDAELEQQLFPPAPAVASEKRPLPDWAWVHAELRRPGVTLALLWQ
EYRLSQPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHARCFAFLG
GVPEIVVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNTAIAGLLERLNQRPFKKLP
GSRQSAFDSLDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVEFFHANQRVASHLRSMHKGRHSTQAEHMPKSHRE
HAEWTPQRLIRWAEQTGPNTAGVIRHILERRIHPQQGYRACLGILRLGKTHGEARLELACRRAISLGTCSYKSLESILRQGLENLPLAQTNLPLLPDDHA
NLRGSAYYH
EYRLSQPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHARCFAFLG
GVPEIVVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNTAIAGLLERLNQRPFKKLP
GSRQSAFDSLDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVEFFHANQRVASHLRSMHKGRHSTQAEHMPKSHRE
HAEWTPQRLIRWAEQTGPNTAGVIRHILERRIHPQQGYRACLGILRLGKTHGEARLELACRRAISLGTCSYKSLESILRQGLENLPLAQTNLPLLPDDHA
NLRGSAYYH
Blast result :
Comments
ISPpu7 is 96% (for ORF1) and 86% (for ORF2) aa similar to IS1491.
Inverted repeats :
L1 : TGCGGATTCCACGC
L2 : CCACGCTGATCCGGACAC
R1 : TGCGGATTCCACGA
R2 : CCACGCTGATCCGGACAC
Inverted repeats :
L1 : TGCGGATTCCACGC
L2 : CCACGCTGATCCGGACAC
R1 : TGCGGATTCCACGA
R2 : CCACGCTGATCCGGACAC
References
1] Wery,J., Hidayat,B., Kieboom,J. and de Bont,J.A. (2001) J. Biol. Chem. 276 (8), 5700-5706
2] Wery,J. and De Bont,J.A.M. (2000) Direct submission GenBank
2] Wery,J. and De Bont,J.A.M. (2000) Direct submission GenBank