ISPpu5
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ233397 | ND | Pseudomonas putida | Pseudomonas putida P1 |
DNA section
IS Length : 1355 bp
Ends
IR Length : 13
IRL : ACCCCCGGCACCTTGTGAGGGCTACGCTTTCACAGGGTGTTTTATTTCGG
IRR : TCTATGGTCACCCCCGTTTTTGCAATACTGATTAGCGGGTGAAGTGGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAAACGCCG | AGATTTATGG | 0 |
DNA sequence
ACCCCCGGCACCTTGTGAGGGCTACGCTTTCACAGGGTGTTTTATTTCGGAGACCTTAATCATGAGCGAGTTAGCATTAGTCGGTATCGATCTCGGCAAA
AATACATTCCACCTGCACGGTCAGGACAAGCCAGGGCGAGAGCTGTTCACAAGAAACTCTCTCGCCAGCAGATGATGAAGTTTTTTTGCAATCTCCCTGC
ATGCACCGTAGTTATGGAGGCTTGCGCTGGCTCGCATTTTGTGGCTAGAGAGCTGATGGCGATGGGGCATAAGGCCAAGCTGATTTCGCCGCAGTTCGTC
CGTCCATTTGTCAAAAGCAACAAGAACGACTTTGTAGATGCTGAGGCGATCTGCGAGGCTGCCGCACGTCCGACAATGCGTTTCGTCACACCCAAGACAG
AGGTTCAGCAACTGCTTTCAATGATGCATCGCATGCGGGAATCTCTGGTGCACGATCGCACTAAAGTGGTCAATCAGATGCATGGCTTTCTGCTTGAATT
CGGGATTAGCCTGCCGAAAGGCCAACAATGATGAAGCGTTTGGGGAGTATTCTGAGCGAGCATGAACTGCCGATTCGACTGACGGTGTTGCTACAGCGAT
TGCATAAGCACTTCAGTTATCTGGATGAGCAGATCAAGGAAATGGATAAAGAGCTTATCGGCCAACTTGCCGACGATGATCTTGGCAGTCGCTTGCTGAG
CATACCGTGTGTCGGCCCGATCACCGCCAGCCTGCTGGCTGCCGAAATGGGGGATGGCAAACAATACGGATGTAGCCGTGATTTCGCCGCGTCCGTAGGG
CTGGTGCCCCGGCAGCACAGCACGGGCGGCAAGGCGACTCTGCTGGGAATCAGCAAACGAGGCGATAAGAACCTCAGAAGATTGTTGGTGCAGTGCGCCA
GGGTTTACATGCTTAGGCTGAAATATCAGCACGGCGCTCTGGCCGATTGGGTGCGCTCAATGCTCTGTCGTCGCCACTCGAACGTCGTGGCCTGCGCTTT
GGCCAACAAGCTGGCACGAATCGCATGGGCGATAGCGGCTCACCATACGCAATTTGAAACCGGGCCAGAGGCTTTAGCTGCCTGAAGCTGATGTTGTGCT
GTGCTAAGAATCACCCACCTAGGTTTTGCGATGACTGAACAATCGATGACGTGAACGGCCCTCCGGCCTGGCGAAGAACCTGATCCATAAACCGGCTATA
TAAAGCCGCTGGGGTTTTTAGGATCGTCAGGCGCGACTCTCATCGTGGCGCGGGGCATGCCCCAGATAGACGCCGGATAGATTTAAGCAAGCCAACCACT
TCACCCGCTAATCAGTATTGCAAAAACGGGGGTGACCATAGA
AATACATTCCACCTGCACGGTCAGGACAAGCCAGGGCGAGAGCTGTTCACAAGAAACTCTCTCGCCAGCAGATGATGAAGTTTTTTTGCAATCTCCCTGC
ATGCACCGTAGTTATGGAGGCTTGCGCTGGCTCGCATTTTGTGGCTAGAGAGCTGATGGCGATGGGGCATAAGGCCAAGCTGATTTCGCCGCAGTTCGTC
CGTCCATTTGTCAAAAGCAACAAGAACGACTTTGTAGATGCTGAGGCGATCTGCGAGGCTGCCGCACGTCCGACAATGCGTTTCGTCACACCCAAGACAG
AGGTTCAGCAACTGCTTTCAATGATGCATCGCATGCGGGAATCTCTGGTGCACGATCGCACTAAAGTGGTCAATCAGATGCATGGCTTTCTGCTTGAATT
CGGGATTAGCCTGCCGAAAGGCCAACAATGATGAAGCGTTTGGGGAGTATTCTGAGCGAGCATGAACTGCCGATTCGACTGACGGTGTTGCTACAGCGAT
TGCATAAGCACTTCAGTTATCTGGATGAGCAGATCAAGGAAATGGATAAAGAGCTTATCGGCCAACTTGCCGACGATGATCTTGGCAGTCGCTTGCTGAG
CATACCGTGTGTCGGCCCGATCACCGCCAGCCTGCTGGCTGCCGAAATGGGGGATGGCAAACAATACGGATGTAGCCGTGATTTCGCCGCGTCCGTAGGG
CTGGTGCCCCGGCAGCACAGCACGGGCGGCAAGGCGACTCTGCTGGGAATCAGCAAACGAGGCGATAAGAACCTCAGAAGATTGTTGGTGCAGTGCGCCA
GGGTTTACATGCTTAGGCTGAAATATCAGCACGGCGCTCTGGCCGATTGGGTGCGCTCAATGCTCTGTCGTCGCCACTCGAACGTCGTGGCCTGCGCTTT
GGCCAACAAGCTGGCACGAATCGCATGGGCGATAGCGGCTCACCATACGCAATTTGAAACCGGGCCAGAGGCTTTAGCTGCCTGAAGCTGATGTTGTGCT
GTGCTAAGAATCACCCACCTAGGTTTTGCGATGACTGAACAATCGATGACGTGAACGGCCCTCCGGCCTGGCGAAGAACCTGATCCATAAACCGGCTATA
TAAAGCCGCTGGGGTTTTTAGGATCGTCAGGCGCGACTCTCATCGTGGCGCGGGGCATGCCCCAGATAGACGCCGGATAGATTTAAGCAAGCCAACCACT
TCACCCGCTAATCAGTATTGCAAAAACGGGGGTGACCATAGA
Protein section
ORF number : 0
Comments
ISPpu5 is a relative of IS1328. Comparison of the inverted repeats shows that 6 bases of the left inverted repeat (and probably also the direct repeat and the actual target site) were replaced by the end of ISPpu4. ISPpu5 does not encode a functional transposase. The tnpA gene is interupted by several frameshifts.
The IRl end of ISPpu5 has been interrupted by insertion of ISPpu4, resulting in loss of the outer 6 bp of IRl. The length of this element has been therefore been predicted by analogy with other IS1111 family elements, assuming that 7 nt would separate IRl from the left-hand end of the complete element and that 4 nt separate IRr from the right-hand end.
The ISPpu5 transposase gene is interrupted by several frameshifts, suggesting that it does not encode a functional transposase protein.
The IRl end of ISPpu5 has been interrupted by insertion of ISPpu4, resulting in loss of the outer 6 bp of IRl. The length of this element has been therefore been predicted by analogy with other IS1111 family elements, assuming that 7 nt would separate IRl from the left-hand end of the complete element and that 4 nt separate IRr from the right-hand end.
The ISPpu5 transposase gene is interrupted by several frameshifts, suggesting that it does not encode a functional transposase protein.
References