ISPpu2
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ233397 | ND | Pseudomonas putida | Pseudomonas putida P1 |
DNA section
IS Length : 1131 bp
Ends
IR Length : 16/27
IRL : TGTGCATCCAATGTCATGGATTGGTTATACGCATGGCCCGACCGACTTCC
IRR : TGAGCGGTTAATTCATTAACTTATTTTTTATCACGCTCAGCTTCGCTTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACGGTATAC | GGCCAAGCGG | 0 |
DNA sequence
TGTGCATCCAATGTCATGGATTGGTTATACGCATGGCCCGACCGACTTCCCCCTTTGCTTTAATGCCTTCAGATCTCATCGCCCTGCAAGGTTGGTTGCG
TATGAGTACGCTGGAACAGAGCCTCGCCCAGCGAGCGCGCATCCTGCTTCTACTGGCTGATGGATTGACGCCCAAGGCCGTCAGCGAGCAGTTGCAGGTC
ACGCCACCGACGGTATTCAAGTGGCGAAAACGCTATCTGGAGGCGGGAATCGAAGGGTTGAGCGATCTGCCGCGCAGCGGCCAGCCACTCAAACTGGGGC
CCGAAAAAATCAACGAAATCCTGACATTGACCACCCGGCGTGTACCCCAGGAAGCCACTCACTGGAGCGTTCGTTTGATGGCCAAGTATGCGCGGGTCAC
CACGTGGCAGGTTCGGCAGGTCTGGGCCGCTTCCGACCTCAAACCCCATCGCTTGAAGACCTTCAAAATCAGCAACGATCCTCATTTCGCCGACAAGGTA
GTCGACGTGGTCGGGCTCTATCTGAACCCGCCGGATAACGCCCTGGTGTTGTCGGTTGACGAGAAAACCCAGATCCAGGCGTTGGATCGCACCCAACCGA
TGCTGCCGCTACGTCCAGGTCAAGTTGAGCGTCGCACGCATGATTACAAACGCCATGGTACGGCGAGTCTGTACGCAGCTTTCGACATCCTGACCGGCCA
AGTCATCGGACGGATTACCCAGCGCCATCGAGCCAAGGAGTTTCTGGCTTTCCTGCAACAGATTGACCGCAGCACGCCAGCCGGCCTGGATCTGCATGTG
ATTCTGGATAACAGTTCGACCCACAAAACGGCTGCAATCAAGCAGTGGCTGGAAAAGCATCCGTGTTTCAAGTTGCACTTCACACCGACCAGCGCGTCTT
GGCTGAACGCCGTGGAAGGCTGGTTTGCACAGCTGGAAAGACGAGCGTTGTACCAGGCCGCCTTCACCAGCGTCGCCGACCTGAAGGCGGCTATCCGCCA
ATTTATCGAGGCTCACAATGAGCATTCAGCCAAGCCGTTCAAGTGGAATAAAACGGCTGAGGCCATCATTAGCTTGGTACATAAAGCGAAGCTGAGCGTG
ATAAAAAATAAGTTAATGAATTAACCGCTCA
TATGAGTACGCTGGAACAGAGCCTCGCCCAGCGAGCGCGCATCCTGCTTCTACTGGCTGATGGATTGACGCCCAAGGCCGTCAGCGAGCAGTTGCAGGTC
ACGCCACCGACGGTATTCAAGTGGCGAAAACGCTATCTGGAGGCGGGAATCGAAGGGTTGAGCGATCTGCCGCGCAGCGGCCAGCCACTCAAACTGGGGC
CCGAAAAAATCAACGAAATCCTGACATTGACCACCCGGCGTGTACCCCAGGAAGCCACTCACTGGAGCGTTCGTTTGATGGCCAAGTATGCGCGGGTCAC
CACGTGGCAGGTTCGGCAGGTCTGGGCCGCTTCCGACCTCAAACCCCATCGCTTGAAGACCTTCAAAATCAGCAACGATCCTCATTTCGCCGACAAGGTA
GTCGACGTGGTCGGGCTCTATCTGAACCCGCCGGATAACGCCCTGGTGTTGTCGGTTGACGAGAAAACCCAGATCCAGGCGTTGGATCGCACCCAACCGA
TGCTGCCGCTACGTCCAGGTCAAGTTGAGCGTCGCACGCATGATTACAAACGCCATGGTACGGCGAGTCTGTACGCAGCTTTCGACATCCTGACCGGCCA
AGTCATCGGACGGATTACCCAGCGCCATCGAGCCAAGGAGTTTCTGGCTTTCCTGCAACAGATTGACCGCAGCACGCCAGCCGGCCTGGATCTGCATGTG
ATTCTGGATAACAGTTCGACCCACAAAACGGCTGCAATCAAGCAGTGGCTGGAAAAGCATCCGTGTTTCAAGTTGCACTTCACACCGACCAGCGCGTCTT
GGCTGAACGCCGTGGAAGGCTGGTTTGCACAGCTGGAAAGACGAGCGTTGTACCAGGCCGCCTTCACCAGCGTCGCCGACCTGAAGGCGGCTATCCGCCA
ATTTATCGAGGCTCACAATGAGCATTCAGCCAAGCCGTTCAAGTGGAATAAAACGGCTGAGGCCATCATTAGCTTGGTACATAAAGCGAAGCTGAGCGTG
ATAAAAAATAAGTTAATGAATTAACCGCTCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1092 bp | 363 aa | 33 | 1124 | + | No |
Chemistry : DDE
ORF sequence :
MARPTSPFALMPSDLIALQGWLRMSTLEQSLAQRARILLLLADGLTPKAVSEQLQVTPPTVFKWRKRYLEAGIEGLSDLPRSGQPLKLGPEKINEILTLT
TRRVPQEATHWSVRLMAKYARVTTWQVRQVWAASDLKPHRLKTFKISNDPHFADKVVDVVGLYLNPPDNALVLSVDEKTQIQALDRTQPMLPLRPGQVER
RTHDYKRHGTASLYAAFDILTGQVIGRITQRHRAKEFLAFLQQIDRSTPAGLDLHVILDNSSTHKTAAIKQWLEKHPCFKLHFTPTSASWLNAVEGWFAQ
LERRALYQAAFTSVADLKAAIRQFIEAHNEHSAKPFKWNKTAEAIISLVHKAKLSVIKNKLMN
TRRVPQEATHWSVRLMAKYARVTTWQVRQVWAASDLKPHRLKTFKISNDPHFADKVVDVVGLYLNPPDNALVLSVDEKTQIQALDRTQPMLPLRPGQVER
RTHDYKRHGTASLYAAFDILTGQVIGRITQRHRAKEFLAFLQQIDRSTPAGLDLHVILDNSSTHKTAAIKQWLEKHPCFKLHFTPTSASWLNAVEGWFAQ
LERRALYQAAFTSVADLKAAIRQFIEAHNEHSAKPFKWNKTAEAIISLVHKAKLSVIKNKLMN
Blast result :
Comments
ISPpu2 is located next to ISPpu3 (back to back), between alkS and alkB, genes involved in alkane degradation by Pseudomonas putida P1. The missing direct repeats of ISPpu2 and 3, and direct repeats flanking the combination of both insertion sequences suggests that ISPpu2 and ISPpu3 may have moved as a single IS-element, named ISPpu6.
ISPpu2 is 62% aa similar to IS870.1.
ISPpu2 is 62% aa similar to IS870.1.
References