ISPp1

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Z32771 ND Pediococcus pentosaceus
Pediococcus pentosaceus PPE1.0
DNA section
IS Length : 1039 bp

Ends


IR Length : 21/23

IRL : GGTAGATTGTAAAATTAATCCGACGCTGTTCGGACAAAAAGATCAGCTTC
IRR : GGTAGATTGCAAATTTAATCCGAATTTTTGGACAAATTTGTCAGCATAAC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGTAGATTGTAAAATTAATCCGACGCTGTTCGGACAAAAAGATCAGCTTCCTTAAAATGGTGTTTACCACAAACCCATCTTTTAGGAGCTGATCTTTTGT
CTAGTATAACCTATTCCGAACGAATTAAAATCGAAACCTTTTGTGAACTAGGGCTGTCCAATATCCAAATGGGCGTTCGGCTGAACCGATCACCGTCAAC
AATTTCTTATGAATTATCTCGATGTCAACCTTATCAGGCTGAATTAGCACAAACAGATGCCGAATACAAGCGATCACAATGTGGTCGGAAAACTAAGCTG
AGCGATGAGTTAAAGCAAAAAATTCTCAACCATTTACGTCTAAGCTGGTCACCAGGAATGATTGCTCACGAATTTAAACTAGCTACTAAATCTATTTATA
ATTGGCTAAATCAGGGGAGAATTGGTTTCTCCTTGAATGATCTACCTGAACATGGCGTACGCCAACGGCGTAACGTTGACCAACGATCCAAATATAATCA
ATCTTTGGGGCGATCAATTGAACAGCGTCCCATGATGTTTAATCAACGTAATCGCATCGGCGATTTTGAACTAGATACAGTCGTTGGTCCTCGTGGGCAT
AGTAAGGCAGTTTTATTAACTTTAATCGATCGCAAATCACGGTTCCTTTGGGCCTACCGGTTAAAAGATCGGACGACAGCGACTGTTAATGAAGCCCTAA
CTAAGTTCCTAACCACTTTTAATGGTCCGGTGCACAGCTTCACGGTGGACCGTGGCACTGAGTTTAGCGGTCTAGTATCACTTGAATCACAATATGGTAT
TAAGACCTATTACTGCCATGCTTATACTCCAGCTGACGTGGTAGTAATGAACGCTTTAATCGGAATTTACGTTGTTTTTATCCTAAAGGGACTCGTTTTG
AGCACATTAGTACTCAAGATTTAACGACGACGTTACTCCAAATTAACCAGCGACCGCTTAAAATACTCGACTAGCAAACACCGTATCAGGTTATGCTGAC
AAATTTGTCCAAAAATTCGGATTAAATTTGCAATCTACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
883 bp293 aa94976+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LLSSITYSERIKIETFCELGLSNIQMGVRLNRSPSTISYELSRCQPYQAELAQTDAEYKRSQCGRKTKLSDELKQKILNHLRLSWSPGMIAHEFKLATKS
IYNWLNQGRIGFSLNDLPEHGVRQRRNVDQRSKYNQSLGRSIEQRPMMFNQRNRIGDFELDTVVGPRGHSKAVLLTLIDRKSRFLWAYRLKDRTTATVNE
ALTKFLTTFNGPVHSFTVDRGTEFSGLVSLESQYGIKTYYCHAYTPADRGSNERFNRNLRCFYPKGTRFEHISTQDLTTTLLQINQRPLKILD

 

Blast result :
Comments
This element has been found by Vaughan and de Vos (1995) through its similarities to IS1070. However, contrary to these authors' suggestion, the element is apparently complete: its coordinates are (9404-10442) CS. ISPp1 is associated with the sucrose and raffinose operons and is located between the alpha-galactosidase and fructose 6-phospohotransferase genes. (Note that on the same 22-kb fragment is another short sequence (30-bp) nearly identical to the 5' end of IS1070). Finally, the ISPp1 ORF considered here is a fusion between two overlapping ORFs: (94-837) and (837-974). The last 45 aa contain a highly conserved domain. The reason for this frameshift remains to be determined.
References
1] Leenhouts, K.K.J., Bolhuis, A.A., Kok, J.J., and Venema, G. (1994) EMBL Data Library, Rel. 39.
2] Vaughan, E.E., and de Vos, W.M. (1995) Gene 155, 95-100.